FastQCFastQC Report
Thu 26 May 2016
ERR580132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8014778
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104860.1308333181530418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85720.10695243211976675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87850.043.1866231
GTATCAA147650.037.2537421
CAACGCA162450.032.37395
ATCAACG166050.031.6441213
GTACATG261800.029.3786031
AACGCAG185800.029.11326
TATCAAC188100.028.9423982
GTACTAG45950.028.54541
TACATGG267500.028.5063652
ACATGGG262950.027.8831333
TCAACGC191700.027.851184
CTGTGCG57850.027.7013199
TAGTACT59700.027.5537764
CATGGGG206700.025.9210014
CTAGTAC63950.025.28183
ACGCAGA210500.024.9379867
ATGGGGG151800.024.7377225
TCTAACG17800.024.0345572
CGCAGAG220500.023.7645328
GAGTACA187050.023.5755921
Produced by FastQC (version 0.11.5)