FastQCFastQC Report
Thu 26 May 2016
ERR580131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8170180
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247470.3028941834818817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196990.24110851902895652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111570.13655757890279038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137800.049.1392171
GTATCAA259400.042.056481
CAACGCA300400.035.0201535
ATCAACG301050.034.9586523
TATCAAC327200.032.9740682
AACGCAG333450.032.2381676
TCAACGC341250.031.0208444
GTACTAG55000.030.5225241
ACGCAGA359350.029.157187
CGCAGAG364450.028.6078
GCATCGT55000.026.7383528
GTACATG347300.026.1452271
GCAGAGT399400.025.6477559
TACATGG352850.025.3409862
TAGGCAT69750.025.2723085
ACATGGG346400.025.2119883
CTGTGCG66050.024.757539
GTGGTAT53800.024.735411
CTAGGCA77400.023.4425164
TAGTACT70950.023.2547994
Produced by FastQC (version 0.11.5)