FastQCFastQC Report
Thu 26 May 2016
ERR580131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8170180
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219010.26806018961638545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183570.2246829323221765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107830.13197995637795004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143000.048.888761
GTATCAA256550.039.4187161
CAACGCA298700.032.6504635
ATCAACG299750.032.600213
TATCAAC324300.030.9679412
AACGCAG333450.029.880676
GTACTAG51800.029.5884321
GTGGTAT54400.029.2113671
TCAACGC338300.029.135084
ACGCAGA358500.027.1241767
CGCAGAG361950.026.7487748
GTACATG339700.026.296151
CTGTGCG64700.025.6398689
TACATGG346200.025.5186522
TAGGCAT67300.025.4908475
TAGTACT68550.025.1639254
ACATGGG337300.025.1527773
GCAGAGT398200.023.910229
CATGGGG246550.023.0102864
CTAGGCA75400.022.8154744
Produced by FastQC (version 0.11.5)