FastQCFastQC Report
Thu 26 May 2016
ERR580130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3439935
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102100.2968079338708435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87730.25503388872173455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44410.12910127662295945No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG37450.10886833617495681No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC35350.10276356966047323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64550.052.0054741
GTATCAA112950.042.291641
TATCAAC138200.034.2180182
ATCAACG136900.034.0528143
CAACGCA136900.033.9513175
TCAACGC144800.032.2283554
AACGCAG147250.032.1926
GTGGTAT24100.029.4582541
ACGCAGA161650.028.947747
CGCAGAG166750.027.9496848
GTACATG155150.027.9096851
TACATGG155450.027.4271982
ACATGGG160150.025.8519763
TACCTGG20400.025.5774782
GCAGAGT181650.025.143839
CATGGGG135950.024.854624
ATGGGGG106500.024.5351645
TGGTATC28100.024.0891282
TGGGGGG105750.022.9679536
GAGTACT94300.022.67353412-13
Produced by FastQC (version 0.11.5)