FastQCFastQC Report
Thu 26 May 2016
ERR580130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3439935
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88780.2580862719789764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82540.2399463943359395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40940.11901387671569374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60100.055.3094061
GTATCAA105100.040.9776271
TATCAAC132350.032.3279232
ATCAACG131550.031.9415133
CAACGCA132500.031.889865
TACCTGG21100.030.3051782
TCAACGC139450.030.3005144
AACGCAG142450.030.2527716
GTGGTAT22650.029.6839621
GTACATG154700.028.4775961
TACATGG158300.027.3551482
ACGCAGA158300.026.5408527
ACATGGG160950.025.9316883
ATGGGGG106400.025.6209495
CGCAGAG164400.025.4988948
TGGGGGG95750.025.2279876
CATGGGG137450.024.8598484
TGGTATC27550.023.7221222
GCAGAGT176900.023.2972589
GAGTACT91000.022.12769512-13
Produced by FastQC (version 0.11.5)