##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580130_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3439935
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52050489326106	34.0	31.0	34.0	31.0	34.0
2	32.637678618927396	34.0	31.0	34.0	31.0	34.0
3	32.68255766460703	34.0	31.0	34.0	31.0	34.0
4	36.213022339084894	37.0	35.0	37.0	35.0	37.0
5	36.15334504867098	37.0	35.0	37.0	35.0	37.0
6	36.19029720038315	37.0	36.0	37.0	35.0	37.0
7	36.14253321647066	37.0	36.0	37.0	35.0	37.0
8	36.12940273580751	37.0	36.0	37.0	35.0	37.0
9	37.83701668781532	39.0	38.0	39.0	35.0	39.0
10-11	37.78447572410525	39.0	38.0	39.0	35.0	39.0
12-13	37.74511320708095	39.0	38.0	39.0	35.0	39.0
14-15	39.11503429570617	40.0	38.0	41.0	36.0	41.0
16-17	39.09221307960761	40.0	38.0	41.0	35.5	41.0
18-19	39.08472878121243	40.0	38.5	41.0	35.0	41.0
20-21	39.05939618045109	40.0	39.0	41.0	35.0	41.0
22-23	38.99143864636977	40.0	38.0	41.0	35.0	41.0
24-25	38.92202076492724	40.0	38.0	41.0	35.0	41.0
26-27	38.79869735910708	40.0	38.0	41.0	35.0	41.0
28-29	38.6842241205139	40.0	38.0	41.0	34.5	41.0
30-31	38.52155912829748	40.0	38.0	41.0	34.0	41.0
32-33	38.39790824535929	40.0	38.0	41.0	34.0	41.0
34-35	38.316552638349265	40.0	38.0	41.0	34.0	41.0
36-37	38.21196824940006	40.0	38.0	41.0	34.0	41.0
38-39	38.10606915537648	40.0	37.5	41.0	33.5	41.0
40-41	37.99473202255275	40.0	37.0	41.0	33.0	41.0
42-43	38.008168759002714	40.0	37.0	41.0	33.0	41.0
44-45	37.997769725300046	40.0	37.0	41.0	33.0	41.0
46-47	37.83006132383315	40.0	37.0	41.0	33.0	41.0
48-49	37.65157771876504	40.0	37.0	41.0	33.0	41.0
50-51	37.47192621372206	40.0	36.5	41.0	32.5	41.0
52-53	37.316233882326266	40.0	36.0	41.0	32.0	41.0
54-55	37.10974146313811	39.0	35.5	41.0	32.0	41.0
56-57	36.89153327025075	39.0	35.0	41.0	31.0	41.0
58-59	36.66516692902627	39.0	35.0	41.0	31.0	41.0
60-61	36.400775741402086	38.0	35.0	40.0	31.0	41.0
62-63	36.07827328132654	38.0	35.0	40.0	30.5	41.0
64-65	35.76681594274311	37.0	35.0	40.0	30.0	41.0
66-67	35.43834709086073	36.5	34.5	39.5	30.0	41.0
68-69	35.10435168106375	36.0	34.0	39.0	29.0	41.0
70-71	34.749732189707075	35.5	34.0	39.0	29.0	40.5
72-73	34.40842472314157	35.0	34.0	38.0	29.0	40.0
74-75	34.04521466248636	35.0	34.0	37.0	29.0	39.0
76-77	32.51748158032056	34.0	31.5	35.5	26.5	38.5
78-79	33.39711244543865	35.0	33.0	36.0	28.5	39.0
80-81	33.38897319280742	35.0	33.5	36.0	29.0	37.5
82-83	33.17048258179297	35.0	33.0	35.5	28.5	37.0
84-85	32.96189797190935	35.0	33.0	35.0	28.0	37.0
86-87	32.767642702551065	35.0	33.0	35.0	27.0	36.0
88-89	32.60833562262078	35.0	33.0	35.0	27.0	36.0
90-91	32.462758162581565	35.0	33.0	35.0	27.0	36.0
92-93	32.31431596817963	35.0	33.0	35.0	26.0	35.0
94-95	32.184891138931405	35.0	33.0	35.0	26.0	35.0
96-97	32.06600284598401	35.0	32.5	35.0	25.0	35.0
98-99	31.971580567656076	35.0	32.0	35.0	25.0	35.0
100	30.113584704362147	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.05778756113641492
1101	2	-0.09877958415906107
1101	3	-0.05399057048614253
1101	4	0.01676156538479745
1101	5	-0.04733690154935033
1101	6	-0.05235950017784319
1101	7	-0.06220898071237713
1101	8	-0.05869468356293339
1101	9	-0.048680638722807146
1101	10-11	-0.021595682264056393
1101	12-13	-0.029336240206575326
1101	14-15	-0.11508481158517014
1101	16-17	-0.06474970798564783
1101	18-19	-0.008712299296909976
1101	20-21	-0.049951700398047194
1101	22-23	-0.10755394155390974
1101	24-25	-0.18481421186035618
1101	26-27	-0.12272154207528985
1101	28-29	-0.17603318693524983
1101	30-31	-0.20651915281452915
1101	32-33	-0.2251176308404368
1101	34-35	-0.29283797883748974
1101	36-37	-0.19639976995855335
1101	38-39	-0.2579638978312033
1101	40-41	-0.2439555864908769
1101	42-43	-0.210500988857234
1101	44-45	-0.13991979480091743
1101	46-47	-0.2034456015942112
1101	48-49	-0.14179976034233732
1101	50-51	-0.08555479729142945
1101	52-53	-0.11114982738833135
1101	54-55	-0.14892797262020707
1101	56-57	-0.1548232167878396
1101	58-59	-0.21823977893539848
1101	60-61	-0.2804436234352252
1101	62-63	-0.19797094838814644
1101	64-65	-0.2063298288408717
1101	66-67	-0.24368580617784374
1101	68-69	-0.24757799500549993
1101	70-71	-0.31573495715762334
1101	72-73	-0.27502539330622255
1101	74-75	-0.2560965542101954
1101	76-77	-0.18911935914334776
1101	78-79	-0.16354466904828513
1101	80-81	-0.1591295107468369
1101	82-83	-0.13701032229823085
1101	84-85	-0.05494251636476832
1101	86-87	-0.19680502566600921
1101	88-89	-0.28564044185194604
1101	90-91	-0.20189991474121882
1101	92-93	-0.21115687136610006
1101	94-95	-0.198044821769777
1101	96-97	-0.37847894716419717
1101	98-99	-0.36793549375908796
1101	100	-0.5223295066765239
1102	1	-0.049969213173909566
1102	2	-0.11667902543675979
1102	3	-0.07286205870893525
1102	4	-0.06396867911855253
1102	5	-0.03873688818594445
1102	6	0.033426160129913285
1102	7	0.02603997021284954
1102	8	0.003987239977654156
1102	9	-0.06804873762710173
1102	10-11	0.00965841733852102
1102	12-13	-0.02870590131237094
1102	14-15	-0.050783327399635425
1102	16-17	0.038361284548720676
1102	18-19	-0.042127271774866415
1102	20-21	-0.028719970391641425
1102	22-23	-0.03994482507297192
1102	24-25	-0.10114208784038681
1102	26-27	-0.12466648294219596
1102	28-29	-0.07957627875787665
1102	30-31	-0.008392053606264938
1102	32-33	-0.08462532091848374
1102	34-35	-0.06584407801093306
1102	36-37	-0.049298783042459604
1102	38-39	-0.2152913286779139
1102	40-41	-0.13267945608281195
1102	42-43	-0.057012809358397476
1102	44-45	0.021616451567666672
1102	46-47	-0.021596594956733384
1102	48-49	-0.058087404928329534
1102	50-51	-0.027275176398205758
1102	52-53	-0.03643236470471578
1102	54-55	-0.09515487956476676
1102	56-57	-0.07034971163115955
1102	58-59	-0.07902503928191607
1102	60-61	-0.19629281640757057
1102	62-63	-0.11596049857763546
1102	64-65	-0.05535481747726578
1102	66-67	-0.10919837295960377
1102	68-69	-0.16510542528754968
1102	70-71	-0.16661684813666255
1102	72-73	-0.15192175064758118
1102	74-75	-0.12905098780392876
1102	76-77	-0.17595211794414212
1102	78-79	-0.13847627567842125
1102	80-81	-0.12918436660551436
1102	82-83	-0.03088296335044305
1102	84-85	-0.20739084372691963
1102	86-87	-0.10175409883821374
1102	88-89	-0.13671422011154988
1102	90-91	-0.115510877631408
1102	92-93	-0.23869439008193893
1102	94-95	-0.2617667386098361
1102	96-97	-0.23294467895678395
1102	98-99	-0.1906319288340832
1102	100	-0.051089542174256763
1103	1	0.024350314444085086
1103	2	0.03210539918493538
1103	3	0.01719208758176194
1103	4	0.022569012175857495
1103	5	0.01785451937977456
1103	6	0.03290281131397421
1103	7	0.007234232993134526
1103	8	-0.024816543127421653
1103	9	-0.05618486425981928
1103	10-11	0.02861190236975375
1103	12-13	0.005922789314745103
1103	14-15	0.06168786272849047
1103	16-17	0.01658408482355611
1103	18-19	0.06663271785139813
1103	20-21	0.04053550812194118
1103	22-23	0.037303210680285304
1103	24-25	-0.017183466360762623
1103	26-27	0.00547878940373181
1103	28-29	0.0819616931907774
1103	30-31	-0.07349104273723839
1103	32-33	-0.05153585234062774
1103	34-35	-0.03627896080305959
1103	36-37	-0.07572827879526045
1103	38-39	0.008646975337050833
1103	40-41	0.0469755085385799
1103	42-43	0.07014030817750694
1103	44-45	0.005056328863730641
1103	46-47	0.06578510452174413
1103	48-49	0.0815342293850847
1103	50-51	0.061445481676067004
1103	52-53	0.004203230587670248
1103	54-55	0.03184050077121725
1103	56-57	0.0726854394093408
1103	58-59	0.03653548813495888
1103	60-61	-0.017197590517056938
1103	62-63	0.11096339579209769
1103	64-65	0.07543144062531582
1103	66-67	0.06733833271515266
1103	68-69	-0.040694061892061484
1103	70-71	0.003289805309762528
1103	72-73	0.07124546771873241
1103	74-75	0.0020837572488048295
1103	76-77	-0.015500658475467333
1103	78-79	0.048667543163922744
1103	80-81	0.009484184916040306
1103	82-83	0.028425605432779832
1103	84-85	0.01773015630790553
1103	86-87	0.029449097309424133
1103	88-89	0.050787789658258475
1103	90-91	0.017622886748796418
1103	92-93	-0.06045863214328051
1103	94-95	0.01082716497106162
1103	96-97	-0.035558978014719855
1103	98-99	-0.08129393416670538
1103	100	0.11903316747303805
1104	1	0.0031086952143866142
1104	2	-0.009590926788590082
1104	3	-0.035752911339393734
1104	4	0.010239163407852914
1104	5	-0.02668354453236077
1104	6	-0.04079527952271178
1104	7	0.02503304289328412
1104	8	0.023601603278805783
1104	9	0.06508442504225798
1104	10-11	0.05097860394659648
1104	12-13	-0.029281374242621894
1104	14-15	-0.08591136422990786
1104	16-17	-0.009398991271289958
1104	18-19	-0.07743373620015603
1104	20-21	-0.059280066544594945
1104	22-23	-0.07169411478499654
1104	24-25	-0.03899882740861216
1104	26-27	-0.03760847741676798
1104	28-29	-0.05089739589036668
1104	30-31	-0.16284611141421834
1104	32-33	-0.1270685191549603
1104	34-35	-0.058474158110030316
1104	36-37	-0.035771816148781
1104	38-39	-0.08756297743028085
1104	40-41	-0.10190166716422766
1104	42-43	-0.03910423791501927
1104	44-45	-0.05069949421697828
1104	46-47	0.009216204249412385
1104	48-49	-0.07266199802276674
1104	50-51	-0.06300541792386838
1104	52-53	-0.14457197444684056
1104	54-55	-0.07814857456808255
1104	56-57	-0.09040454782371654
1104	58-59	-0.16432665684045134
1104	60-61	-0.1350340166619759
1104	62-63	-0.15831730418904755
1104	64-65	-0.12917888805356625
1104	66-67	-0.22582689059165517
1104	68-69	-0.21720600554260727
1104	70-71	-0.10787157429424354
1104	72-73	-0.1429230430220585
1104	74-75	-0.1713929013247295
1104	76-77	-0.08872581329526241
1104	78-79	-0.11069119574027297
1104	80-81	-0.03951603658791214
1104	82-83	-0.08521694095939836
1104	84-85	-0.11328781561915235
1104	86-87	-0.11465481169761915
1104	88-89	-0.09571592465469081
1104	90-91	-0.10676242187645357
1104	92-93	-0.05264972194986228
1104	94-95	-0.08108308435349443
1104	96-97	-0.11109361614249735
1104	98-99	-0.12781056954325365
1104	100	-0.0864579798959042
1105	1	0.03551322786012889
1105	2	0.03478322393204536
1105	3	-0.007324328772732258
1105	4	0.05685555087973171
1105	5	0.04245023228259015
1105	6	0.04040000460996396
1105	7	0.05844870527550228
1105	8	0.02666248964801099
1105	9	-0.010563394119714076
1105	10-11	0.010638704245089059
1105	12-13	0.0415177210660147
1105	14-15	0.04805288389332674
1105	16-17	-0.015243007889324645
1105	18-19	0.025301047776586927
1105	20-21	-0.004583690879094604
1105	22-23	-0.05007299362485895
1105	24-25	-0.043877136813620154
1105	26-27	-0.013298203642513329
1105	28-29	0.007758918849781082
1105	30-31	-0.04180204773775387
1105	32-33	-0.02551611304906487
1105	34-35	-0.04565125791845759
1105	36-37	-0.014456122301460539
1105	38-39	-0.035098048491299494
1105	40-41	-0.050618942063429984
1105	42-43	0.033170306258639926
1105	44-45	-0.04075004722314901
1105	46-47	-0.11824061476776393
1105	48-49	-0.09250119880911001
1105	50-51	-0.09679507648571217
1105	52-53	-0.25914776636504655
1105	54-55	-0.186802905335945
1105	56-57	-0.16164185736237613
1105	58-59	-0.1862793102119511
1105	60-61	-0.16701669414744202
1105	62-63	-0.10115854046134842
1105	64-65	-0.10647510973496566
1105	66-67	-0.10504126541065517
1105	68-69	-0.1264063811746894
1105	70-71	0.03893297728625811
1105	72-73	0.00874519922026451
1105	74-75	-0.042691226712882724
1105	76-77	-0.12032022392322261
1105	78-79	0.02680782967685502
1105	80-81	-0.036231170189296336
1105	82-83	0.0379221278405808
1105	84-85	0.014110528425305802
1105	86-87	0.014300950593266748
1105	88-89	2.4411605502194789E-4
1105	90-91	-0.0510114083102593
1105	92-93	-0.030984570527529343
1105	94-95	0.04851155182079481
1105	96-97	-0.007703425179947487
1105	98-99	0.025824067689153907
1105	100	0.006691259372544778
1106	1	0.04456392706279644
1106	2	0.016731058099381357
1106	3	0.0392056259933824
1106	4	0.05530030225357052
1106	5	0.035286225398714066
1106	6	0.016315430059499647
1106	7	0.031908176354114914
1106	8	-0.011604570523203961
1106	9	-0.03409639696619138
1106	10-11	-0.005296252350134978
1106	12-13	0.03814199375906213
1106	14-15	0.039794537053019496
1106	16-17	0.07800398541905196
1106	18-19	0.12088045507649525
1106	20-21	0.07448482217036911
1106	22-23	-0.004956764699734606
1106	24-25	0.06004251863180343
1106	26-27	0.0517819169455791
1106	28-29	0.11312905767562143
1106	30-31	0.04634666233546625
1106	32-33	0.09894587506569508
1106	34-35	0.018253497580907663
1106	36-37	0.013684089729046889
1106	38-39	0.09729059243602478
1106	40-41	0.03943682091794898
1106	42-43	0.0730180240702154
1106	44-45	0.12917851381055812
1106	46-47	0.0991283281237898
1106	48-49	0.12400425482482547
1106	50-51	0.11771170158786504
1106	52-53	0.16189862440339908
1106	54-55	0.13229254877862928
1106	56-57	0.08473992404901054
1106	58-59	-0.006011700304824785
1106	60-61	0.08822807685267264
1106	62-63	0.04784363211366838
1106	64-65	0.027283490110683317
1106	66-67	-0.035995169036311836
1106	68-69	0.13797282612414818
1106	70-71	0.11600690358226018
1106	72-73	0.027824782088529787
1106	74-75	0.08551564955881474
1106	76-77	0.10106367699213337
1106	78-79	0.16726101634273505
1106	80-81	0.17964110249306486
1106	82-83	0.11254130971872911
1106	84-85	0.13565794823795585
1106	86-87	0.16340930697968048
1106	88-89	0.07407507610020758
1106	90-91	0.14204019009613944
1106	92-93	0.18208620187979818
1106	94-95	0.13147181629483384
1106	96-97	0.1972089258205898
1106	98-99	0.05158088080965584
1106	100	0.09939836328231166
1107	1	-0.037904118371614004
1107	2	-0.014491906651045383
1107	3	0.01660035942681759
1107	4	0.03302613503920071
1107	5	-0.04728600319337062
1107	6	-0.029849702244312937
1107	7	-0.0413101157457163
1107	8	2.4204816966744147E-5
1107	9	-0.06703458589031186
1107	10-11	-0.035787416371043435
1107	12-13	-0.040236123140353186
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2316	34-35	-0.21773505595799492
2316	36-37	-0.20884326008642518
2316	38-39	-0.2304377274091607
2316	40-41	-0.17421043461448704
2316	42-43	-0.24879945543660398
2316	44-45	-0.39231030895932406
2316	46-47	-0.32891144162078234
2316	48-49	-0.2650678223305789
2316	50-51	-0.21744932739637335
2316	52-53	-0.29006586381521515
2316	54-55	-0.34039416766558617
2316	56-57	-0.4675118001651484
2316	58-59	-0.49899450329098727
2316	60-61	-0.3630419212992422
2316	62-63	-0.20598826613973387
2316	64-65	-0.2638477589184518
2316	66-67	-0.32537595619771054
2316	68-69	-0.2584412443720083
2316	70-71	-0.2306812483511962
2316	72-73	-0.2893707581938898
2316	74-75	-0.21417868731944623
2316	76-77	-0.24697366138484966
2316	78-79	-0.2833451908282001
2316	80-81	-0.30994173837576966
2316	82-83	-0.370173800309864
2316	84-85	-0.35253978825704735
2316	86-87	-0.34124214174087086
2316	88-89	-0.336162140340015
2316	90-91	-0.35688231036894535
2316	92-93	-0.3087195370686828
2316	94-95	-0.2893598081227253
2316	96-97	-0.29846764518977054
2316	98-99	-0.22199394483488888
2316	100	-0.37762759218068354
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	24.0
14	129.0
15	434.0
16	1070.0
17	2604.0
18	4674.0
19	7286.0
20	10424.0
21	15016.0
22	20121.0
23	22108.0
24	21695.0
25	21860.0
26	24048.0
27	29357.0
28	36075.0
29	44529.0
30	55484.0
31	69639.0
32	88504.0
33	118966.0
34	186442.0
35	264239.0
36	442059.0
37	850950.0
38	911576.0
39	190551.0
40	69.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.822169395401005	19.519930367052734	14.009066353930852	23.648833883615413
2	16.0313259437637	20.670429401325052	39.758364155517704	23.53988049939354
3	18.457688605649775	26.65027854096455	30.419033869988247	24.472998983397428
4	13.456387369625494	17.3376943153547	36.997695861912185	32.20822245310762
5	13.580864255316921	37.79464240548098	33.45057298968101	15.173920349521088
6	30.310104967191208	35.75771265245941	19.02607377122571	14.90610860912367
7	27.089891778925164	33.07038018223189	22.299727637668852	17.5400004011741
8	26.775740386057823	34.622315021228644	20.355485316708588	18.246459276004945
9	27.966118693877217	15.852624400017326	20.34029566634524	35.84096123976021
10-11	25.78740815570107	25.591677144123782	27.968836529866202	20.652078170308947
12-13	25.85314236045501	23.449794036506557	27.84034849546357	22.856715107574864
14-15	23.84817234503135	24.690867953511038	25.883049094112398	25.57791060734521
16-17	23.068417766918394	27.270346147780515	26.584257103882123	23.076978981418968
18-19	22.61065816759845	26.84399997499932	28.423141733648865	22.122200123753363
20-21	23.839746622566903	26.268489670203802	27.650001344505082	22.241762362724216
22-23	23.727906206030443	25.764416200565137	27.6988005395537	22.808877053850715
24-25	23.113949125058213	25.61573261439478	27.95663867930507	23.313679581241935
26-27	22.890987999833715	25.848077354108547	27.839286903076832	23.421647742980912
28-29	22.88919335253722	26.97288228914027	27.594959082004312	22.542965276318203
30-31	24.207827779888643	25.919502354299297	27.869499403612398	22.003170462199666
32-33	23.902980590362073	25.975024194094637	27.524507331486568	22.597487884056726
34-35	23.6592852224746	26.45134995557296	27.614694514492317	22.274670307460124
36-37	23.482782103358982	26.331749669131195	27.56530707023576	22.62016115727406
38-39	23.474512783943492	26.40746056354529	27.50490493767117	22.613121714840048
40-41	23.527009953468305	25.67367171288148	27.916109222438706	22.883209111211507
42-43	23.270871295855915	26.274770364289047	27.174751060818725	23.279607279036316
44-45	22.587065255152545	26.532361572821717	27.87479232107634	23.0057808509494
46-47	22.622951963450728	25.844982462168765	27.39547180770554	24.136593766674967
48-49	23.062468246924993	26.02723696097906	27.37434504172327	23.53594975037268
50-51	23.162967092309717	26.253820285068674	27.327359455300936	23.255853167320673
52-53	22.964879648999993	26.543779645860248	26.840049282336153	23.651291422803606
54-55	22.680109966319073	26.03564384413482	27.52643664326837	23.757809546277738
56-57	23.18222412413716	26.077661892756804	26.856542574469273	23.88357140863676
58-59	22.699205115726567	26.349198458258254	27.40936394722168	23.542232478793494
60-61	23.3300689702691	26.444102712745305	27.195991592799366	23.029836724186225
62-63	23.469475265690008	26.29764897376808	26.8836394494242	23.349236311117714
64-65	22.851390485587412	25.992961557475258	26.99637059866589	24.15927735827143
66-67	22.904138979111416	25.76076062617058	27.074137358376476	24.26096303634153
68-69	22.21003988242548	25.989262356821975	27.448972502086566	24.351725258665972
70-71	22.60713743744611	25.72548559029302	27.42766075339682	24.23971621886405
72-73	22.48211399769922	26.42252916750613	26.864064638176398	24.231292196618252
74-75	22.75754530175538	26.31304516700357	26.79092711860514	24.138482412635913
76-77	22.417981178708082	25.96385315374598	26.88887018611249	24.72929548143345
78-79	22.097463204229626	26.083283439901056	27.239212153269715	24.580041202599602
80-81	22.154005552266497	26.280732931737123	26.87097557703352	24.694285938962864
82-83	22.622974553650664	26.050387431821314	27.111796687362354	24.21484132716567
84-85	22.74978945406901	26.43568777513782	26.471125191758865	24.343397579034306
86-87	21.952406825820297	26.766843390978774	26.709253184976777	24.57149659822415
88-89	21.832222332205546	25.913688868229467	27.107322839761615	25.146765959803375
90-91	22.504259951600147	25.558532236456056	27.1203424880443	24.816865323899496
92-93	22.364681495745337	25.93351813357857	26.874409235884272	24.82739113479182
94-95	21.772416702205934	26.030398938095388	26.84767128400977	25.3495130756889
96-97	21.564925191224496	25.85799378203336	27.04020398442325	25.53687704231889
98-99	21.361172026360737	26.02529122673598	26.814226682056702	25.79931006484658
100	21.03314221526989	26.275628042755482	26.862063883220983	25.829165858753644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	620.0
1	430.5
2	429.5
3	575.0
4	433.5
5	387.5
6	562.0
7	952.0
8	1576.0
9	2112.0
10	2051.0
11	1803.5
12	1878.5
13	2129.0
14	2427.0
15	2786.0
16	3115.0
17	3414.0
18	3762.5
19	4069.5
20	4454.5
21	4910.0
22	5457.0
23	6247.0
24	7164.5
25	8428.0
26	10218.5
27	12346.5
28	15190.0
29	19667.0
30	25275.5
31	30852.5
32	37693.5
33	44921.0
34	51941.5
35	59040.0
36	67167.5
37	78259.5
38	85290.0
39	87430.5
40	89990.0
41	91871.0
42	95280.5
43	101308.5
44	108075.0
45	119147.5
46	130768.5
47	141439.0
48	151641.5
49	158476.5
50	164606.5
51	168447.0
52	166037.0
53	158130.0
54	149009.5
55	135079.0
56	119823.5
57	105082.5
58	89217.5
59	73565.0
60	56876.5
61	43655.0
62	34378.5
63	26251.5
64	18646.5
65	12749.0
66	8945.0
67	6620.0
68	4984.0
69	3657.5
70	2697.5
71	1984.5
72	1433.0
73	1025.0
74	731.5
75	483.0
76	299.5
77	167.0
78	91.5
79	49.0
80	25.0
81	12.0
82	8.5
83	5.5
84	2.5
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005348938279357023
2	0.032675036010854856
3	0.0013372345698392557
4	0.0010756017192185318
5	0.00747107140105845
6	8.721095020690798E-5
7	9.302501355403517E-4
8	0.007064086966759546
9	0.0012790939363679836
10-11	2.9070316735635996E-4
12-13	1.4535158367817998E-4
14-15	6.250118098161738E-4
16-17	7.267579183908999E-5
18-19	8.285040269656258E-4
20-21	2.1802737551726993E-4
22-23	0.0015988674204599797
24-25	0.0014244455200461638
26-27	0.0019186409045519755
28-29	1.7442190041381595E-4
30-31	0.0016860783706668875
32-33	0.0013372345698392557
34-35	7.412930767587178E-4
36-37	0.003706465383793589
38-39	9.883907690116237E-4
40-41	0.0037500708588970428
42-43	0.0040407740262534035
44-45	0.003779141175632679
46-47	0.0024855120808968774
48-49	0.008939122396208067
50-51	0.006904200224713548
52-53	0.004738461627908667
54-55	0.004491363935655761
56-57	0.0036919302254257713
58-59	0.005494289863035203
60-61	0.00768909877657572
62-63	0.0034302973748050474
64-65	0.003241340316023413
66-67	0.003997168551149949
68-69	0.0021512034384370635
70-71	0.0029361019902992354
72-73	0.006802454116138823
74-75	0.008081548052506807
76-77	0.004665785836069577
78-79	0.0030087777821383253
80-81	0.0022820198637474257
82-83	0.004898348369954664
84-85	0.0019767815380232474
86-87	0.003314016107862503
88-89	5.668711763449019E-4
90-91	0.0014099103616783456
92-93	1.3081642531036198E-4
94-95	0.0022674847053796078
96-97	0.0018750354294485214
98-99	0.0013517697282070737
100	5.814063347127198E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3439935.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.46677197764123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.88849118711894	48.654426186682784
2	13.703877500706726	17.120739820923504
3	3.807617955774878	7.135488078640852
4	1.573560347207954	3.9318094160838895
5	0.8632512141956881	2.696225837829199
6	0.49267113319797184	1.8465345202466292
7	0.3419902555519115	1.495411911849556
8	0.2473763667483766	1.2362242475462555
9	0.17652363406997837	0.9924175438302528
>10	0.8457936217945028	8.958904276577305
>50	0.03786581659933611	1.610647055512879
>100	0.018174211545053356	2.147852923253913
>500	0.0020123907283077325	0.8397595331703095
>1k	6.8844945902879E-4	0.76681607107618
>5k	1.059153013890446E-4	0.5667425767764072
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8878	0.2580862719789764	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8254	0.2399463943359395	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4094	0.11901387671569374	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.814063347127199E-5
2	0.0	0.0	0.0	0.0	5.814063347127199E-5
3	0.0	0.0	0.0	0.0	5.814063347127199E-5
4	0.0	0.0	0.0	0.0	5.814063347127199E-5
5	0.0	0.0	0.0	0.0	5.814063347127199E-5
6	0.0	0.0	0.0	0.0	8.721095020690798E-5
7	0.0	0.0	0.0	0.0	8.721095020690798E-5
8	0.0	0.0	0.0	2.9070316735635994E-5	1.4535158367817998E-4
9	0.0	0.0	0.0	5.814063347127199E-5	1.4535158367817998E-4
10-11	0.0	0.0	0.0	8.721095020690798E-5	1.4535158367817998E-4
12-13	0.0	0.0	0.0	1.1628126694254398E-4	1.8895705878163395E-4
14-15	0.0	0.0	0.0	1.3081642531036198E-4	2.0349221714945195E-4
16-17	0.0	0.0	0.0	1.5988674204599798E-4	2.0349221714945195E-4
18-19	0.0	0.0	0.0	2.0349221714945195E-4	2.6163285062072396E-4
20-21	0.0	0.0	0.0	2.6163285062072396E-4	2.6163285062072396E-4
22-23	0.0	0.0	0.0	3.633789591954499E-4	2.6163285062072396E-4
24-25	0.0	2.9070316735635994E-5	0.0	9.011798188047158E-4	2.6163285062072396E-4
26-27	0.0	2.9070316735635994E-5	0.0	0.0021657385968048815	2.7616800898854193E-4
28-29	0.0	2.9070316735635994E-5	0.0	0.005101840587104117	2.9070316735635996E-4
30-31	0.0	2.9070316735635994E-5	0.0	0.015407267869887076	2.9070316735635996E-4
32-33	0.0	2.9070316735635994E-5	0.0	0.0439397837459138	2.9070316735635996E-4
34-35	0.0	2.9070316735635994E-5	0.0	0.0919930173099201	3.197734840919959E-4
36-37	0.0	4.360547510345399E-5	0.0	0.16320075815386048	3.197734840919959E-4
38-39	0.0	5.814063347127199E-5	0.0	0.28012157206458843	3.197734840919959E-4
40-41	0.0	5.814063347127199E-5	0.0	0.4194992056535952	3.197734840919959E-4
42-43	0.0	8.721095020690798E-5	0.0	0.5551122332253371	3.197734840919959E-4
44-45	0.0	8.721095020690798E-5	0.0	0.7257259221467848	3.3430864245981393E-4
46-47	0.0	8.721095020690798E-5	0.0	0.9226046422388796	3.779141175632679E-4
48-49	0.0	8.721095020690798E-5	0.0	1.1339749152236889	3.779141175632679E-4
50-51	0.0	8.721095020690798E-5	0.0	1.368906679922731	3.779141175632679E-4
52-53	0.0	8.721095020690798E-5	0.0	1.6226178692329942	3.779141175632679E-4
54-55	0.0	8.721095020690798E-5	0.0	1.9160827166792396	3.779141175632679E-4
56-57	0.0	8.721095020690798E-5	0.0	2.2289229302297864	3.779141175632679E-4
58-59	0.0	8.721095020690798E-5	0.0	2.5920402565746157	3.779141175632679E-4
60-61	0.0	8.721095020690798E-5	0.0	2.9575558840501346	3.779141175632679E-4
62-63	0.0	8.721095020690798E-5	0.0	3.360688501381567	3.779141175632679E-4
64-65	0.0	8.721095020690798E-5	0.0	3.751437163783618	3.779141175632679E-4
66-67	0.0	8.721095020690798E-5	0.0	4.21212900825161	3.779141175632679E-4
68-69	0.0	8.721095020690798E-5	0.0	4.703039446966295	3.779141175632679E-4
70-71	0.0	8.721095020690798E-5	0.0	5.210592642012131	3.779141175632679E-4
72-73	0.0	8.721095020690798E-5	0.0	5.702244373803575	4.360547510345399E-4
74-75	0.0	8.721095020690798E-5	0.0	6.2059166815652045	4.360547510345399E-4
76-77	0.0	8.721095020690798E-5	0.0	6.777017588995141	4.360547510345399E-4
78-79	0.0	8.721095020690798E-5	0.0	7.3922617723881405	4.360547510345399E-4
80-81	0.0	8.721095020690798E-5	0.0	8.008465276233418	4.360547510345399E-4
82-83	0.0	8.721095020690798E-5	0.0	8.64827387726803	4.651250677701759E-4
84-85	0.0	8.721095020690798E-5	0.0	9.299143733820552	4.651250677701759E-4
86-87	0.0	8.721095020690798E-5	0.0	9.981162434755309	4.796602261379939E-4
88	0.0	8.721095020690798E-5	0.0	10.512582359841101	4.941953845058119E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6010	0.0	55.309406	1
GTATCAA	10510	0.0	40.977627	1
TATCAAC	13235	0.0	32.327923	2
ATCAACG	13155	0.0	31.941513	3
CAACGCA	13250	0.0	31.88986	5
TACCTGG	2110	0.0	30.305178	2
TCAACGC	13945	0.0	30.300514	4
AACGCAG	14245	0.0	30.252771	6
GTGGTAT	2265	0.0	29.683962	1
GTACATG	15470	0.0	28.477596	1
TACATGG	15830	0.0	27.355148	2
ACGCAGA	15830	0.0	26.540852	7
ACATGGG	16095	0.0	25.931688	3
ATGGGGG	10640	0.0	25.620949	5
CGCAGAG	16440	0.0	25.498894	8
TGGGGGG	9575	0.0	25.227987	6
CATGGGG	13745	0.0	24.859848	4
TGGTATC	2755	0.0	23.722122	2
GCAGAGT	17690	0.0	23.297258	9
GAGTACT	9100	0.0	22.127695	12-13
>>END_MODULE