FastQCFastQC Report
Thu 26 May 2016
ERR580129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7374971
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195990.26575019752620044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154680.20973641794659262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91360.12387845321696858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113200.050.3497051
GTATCAA211200.042.3503841
CAACGCA248200.035.1303445
ATCAACG248400.035.0258943
TATCAAC265550.033.4044382
AACGCAG274600.032.525096
TCAACGC278350.031.477974
ACGCAGA296950.029.2231047
GTACTAG45650.029.1532121
CGCAGAG299450.028.8220378
GCATCGT42200.028.2788098
GTACATG300000.027.1341721
GTGGTAT46400.027.0603891
TACATGG304350.026.3038862
ACATGGG297800.026.027373
GCAGAGT325700.025.8235769
TAGGCAT58350.024.9724485
CATGGGG225800.024.3148144
TCTAACG22250.024.2966522
CTGTGCG54800.023.665179
Produced by FastQC (version 0.11.5)