FastQCFastQC Report
Thu 26 May 2016
ERR580129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7374971
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172220.23351956231421112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146730.19895671454165717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87220.11826487182119089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119100.051.3597831
GTATCAA219300.040.692581
CAACGCA251100.034.3853035
ATCAACG253150.034.1261143
TATCAAC273650.032.4050522
AACGCAG279900.031.4672976
TCAACGC282600.030.7024384
GTACTAG49750.029.6752361
ACGCAGA303400.028.3328887
CGCAGAG307300.027.8819228
GTACATG295050.025.655941
TAGGCAT61150.025.4414545
GTGGTAT44850.025.3694231
GCATCGT50100.025.3293868
TACATGG298600.025.0994952
GCAGAGT337000.024.9624029
ACATGGG290850.024.8869043
CGACGAG26150.023.27450824-25
CATGGGG219250.023.0238534
CTAGGCA69700.022.9276474
Produced by FastQC (version 0.11.5)