##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580129_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7374971
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.522641105978586	34.0	31.0	34.0	31.0	34.0
2	32.638268136918775	34.0	31.0	34.0	31.0	34.0
3	32.69189126303005	34.0	31.0	34.0	31.0	34.0
4	36.21358782292161	37.0	35.0	37.0	35.0	37.0
5	36.15820143563954	37.0	35.0	37.0	35.0	37.0
6	36.193015538637376	37.0	37.0	37.0	35.0	37.0
7	36.138248950402655	37.0	36.0	37.0	35.0	37.0
8	36.130757395520604	37.0	36.0	37.0	35.0	37.0
9	37.84572902049378	39.0	38.0	39.0	35.0	39.0
10-11	37.796590115405195	39.0	38.0	39.0	35.0	39.0
12-13	37.761692147670814	39.0	38.0	39.0	35.0	39.0
14-15	39.13997560125999	40.0	38.5	41.0	36.0	41.0
16-17	39.13453903750944	40.0	38.0	41.0	36.0	41.0
18-19	39.12814043607764	40.0	38.5	41.0	36.0	41.0
20-21	39.10028920791689	40.0	39.0	41.0	35.0	41.0
22-23	39.02687590229168	40.0	38.5	41.0	35.0	41.0
24-25	38.96242141155538	40.0	38.0	41.0	35.0	41.0
26-27	38.842197413386444	40.0	38.0	41.0	35.0	41.0
28-29	38.74274509011629	40.0	38.0	41.0	35.0	41.0
30-31	38.59302450138448	40.0	38.0	41.0	34.0	41.0
32-33	38.489175957437666	40.0	38.0	41.0	34.0	41.0
34-35	38.40236958219903	40.0	38.0	41.0	34.0	41.0
36-37	38.289741139321094	40.0	38.0	41.0	34.0	41.0
38-39	38.17639045631501	40.0	38.0	41.0	34.0	41.0
40-41	38.071734654956614	40.0	37.5	41.0	33.0	41.0
42-43	38.07846885092836	40.0	38.0	41.0	33.0	41.0
44-45	38.0510844042641	40.0	37.5	41.0	33.0	41.0
46-47	37.88121044814956	40.0	37.0	41.0	33.0	41.0
48-49	37.702451643538666	40.0	37.0	41.0	33.0	41.0
50-51	37.527164513596055	40.0	37.0	41.0	33.0	41.0
52-53	37.378591861581555	40.0	36.0	41.0	32.0	41.0
54-55	37.18052321832859	39.5	36.0	41.0	32.0	41.0
56-57	36.963580941538616	39.0	35.0	41.0	31.5	41.0
58-59	36.73877131177872	39.0	35.0	41.0	31.0	41.0
60-61	36.498394298770805	38.5	35.0	40.5	31.0	41.0
62-63	36.20540033309962	38.0	35.0	40.0	31.0	41.0
64-65	35.92166775977831	37.5	35.0	40.0	30.0	41.0
66-67	35.61946318161793	37.0	35.0	40.0	30.0	41.0
68-69	35.30853375559036	36.5	34.0	39.5	29.5	41.0
70-71	34.990809997761346	36.0	34.0	39.0	29.0	41.0
72-73	34.673977565471105	35.5	34.0	39.0	29.0	40.0
74-75	34.316818466133626	35.0	34.0	37.5	29.0	40.0
76-77	32.76636314908899	34.5	31.5	36.0	26.5	39.0
78-79	33.64447704268939	35.0	33.5	37.0	28.5	39.0
80-81	33.61267400237912	35.0	34.0	36.0	29.0	39.0
82-83	33.36793778578926	35.0	33.5	36.0	29.0	37.0
84-85	33.141884693512694	35.0	33.0	35.5	29.0	37.0
86-87	32.931895799997044	35.0	33.0	35.0	28.0	37.0
88-89	32.74626774803589	35.0	33.0	35.0	27.0	36.0
90-91	32.576867692089905	35.0	33.0	35.0	27.0	36.0
92-93	32.41505729310664	35.0	33.0	35.0	27.0	36.0
94-95	32.28005188088197	35.0	33.0	35.0	26.0	35.0
96-97	32.160875547849614	35.0	33.0	35.0	25.5	35.0
98-99	32.06636439655153	35.0	32.0	35.0	25.0	35.0
100	30.206182370073048	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.012410431112044762
1101	2	-0.014975992691248052
1101	3	0.0027476315192629386
1101	4	-0.014275108076702736
1101	5	-0.05645955516933299
1101	6	-0.04070413824217667
1101	7	-0.04291263103608145
1101	8	-0.02798479836479828
1101	9	-0.04174466938447097
1101	10-11	0.015689311181901644
1101	12-13	-0.05490925171456951
1101	14-15	-0.0366677998104592
1101	16-17	-0.012068205868352777
1101	18-19	0.011674654619845626
1101	20-21	-0.009633928690568894
1101	22-23	-0.0609364094307594
1101	24-25	-0.04625723743378529
1101	26-27	-0.08844319155603131
1101	28-29	-0.0977747468010648
1101	30-31	-0.10696093222174596
1101	32-33	-0.07208086420482829
1101	34-35	-0.059499232691322845
1101	36-37	-0.07009176872769984
1101	38-39	-0.037386728903342714
1101	40-41	-0.13604776012433462
1101	42-43	-0.08194531312324926
1101	44-45	-0.0845067375172448
1101	46-47	-0.08697841317454902
1101	48-49	-0.07246054840688743
1101	50-51	-0.12748599458975463
1101	52-53	-0.011461731200910208
1101	54-55	-0.08662827740314327
1101	56-57	-0.1423049682512385
1101	58-59	-0.15065397768620414
1101	60-61	-0.17340039886232717
1101	62-63	-0.17367073831911028
1101	64-65	-0.21875728898324098
1101	66-67	-0.23638561766303212
1101	68-69	-0.19815762003693038
1101	70-71	-0.22335203066209885
1101	72-73	-0.1828678802277821
1101	74-75	-0.2298412238519134
1101	76-77	-0.203423077755005
1101	78-79	-0.11931386310376979
1101	80-81	-0.14242280087101022
1101	82-83	-0.11889884994148048
1101	84-85	-0.17135275528647753
1101	86-87	-0.17649060264376004
1101	88-89	-0.17625721352067814
1101	90-91	-0.18164247536169142
1101	92-93	-0.13077277289774258
1101	94-95	-0.20017913078282845
1101	96-97	-0.31741617684684087
1101	98-99	-0.2848469666462492
1101	100	-0.3976171435195184
1102	1	-0.03513573707282802
1102	2	-0.08609890843568024
1102	3	-0.07375476879315812
1102	4	-0.025028252558094266
1102	5	-0.018536338539888675
1102	6	0.028649191962486498
1102	7	-0.024601858531553944
1102	8	1.93771122226849E-4
1102	9	-0.007855442110880517
1102	10-11	0.015705953052183474
1102	12-13	-0.042957199161762105
1102	14-15	-0.04870084279863107
1102	16-17	-0.007339418428308875
1102	18-19	-0.025782728324017512
1102	20-21	-0.015567171492129717
1102	22-23	-0.030701320823901312
1102	24-25	-0.05970872249881154
1102	26-27	-0.08075450880443924
1102	28-29	-0.07782239247568157
1102	30-31	-0.1351499028776928
1102	32-33	-0.1064166483309279
1102	34-35	-0.0665351380811785
1102	36-37	-0.08650772561859554
1102	38-39	-0.2038688094921568
1102	40-41	-0.13421881858056395
1102	42-43	-0.1340867889908708
1102	44-45	-0.16896936043131916
1102	46-47	-0.06540638882415806
1102	48-49	-0.03615436683963935
1102	50-51	-0.08549564354614603
1102	52-53	-0.09320188281944297
1102	54-55	-0.1147211412582223
1102	56-57	-0.09374801255966503
1102	58-59	-0.09939798799972976
1102	60-61	-0.14982566410600384
1102	62-63	-0.08694870415002498
1102	64-65	-0.12388586962030956
1102	66-67	-0.11371104167015034
1102	68-69	-0.1439316112344997
1102	70-71	-0.1454811138836547
1102	72-73	-0.13345539372156168
1102	74-75	-0.11944834812563698
1102	76-77	-0.12805981346324558
1102	78-79	-0.13703592944714416
1102	80-81	-0.11917424677216815
1102	82-83	-0.06047215571587827
1102	84-85	-0.12137389046173297
1102	86-87	-0.16473894594557947
1102	88-89	-0.15588298778369136
1102	90-91	-0.16024879638780476
1102	92-93	-0.18688291878036978
1102	94-95	-0.19735861113487374
1102	96-97	-0.23253750717575983
1102	98-99	-0.25134521758567985
1102	100	-0.07132914596920159
1103	1	0.0284772568507492
1103	2	-0.009072846779908161
1103	3	0.0061780610131449976
1103	4	0.013663255338549618
1103	5	-0.0063314651344086315
1103	6	0.019924942085104647
1103	7	-0.011258137002272406
1103	8	-0.04020672807714476
1103	9	-0.07440122844668196
1103	10-11	-0.012644282899827886
1103	12-13	0.03934960111192254
1103	14-15	0.026249837435535994
1103	16-17	0.007688591762480712
1103	18-19	0.031661028756857945
1103	20-21	-0.04085292915809191
1103	22-23	-0.035410672384813324
1103	24-25	-0.024024319011822115
1103	26-27	-0.048521575453058574
1103	28-29	-0.0794794810219912
1103	30-31	-0.08935317875499749
1103	32-33	-0.04174839877757819
1103	34-35	-0.060247995201493154
1103	36-37	-0.09917274645316354
1103	38-39	-0.04959452761212191
1103	40-41	-0.07135588313869334
1103	42-43	0.028341045673165866
1103	44-45	-0.009365877277282664
1103	46-47	-0.018294603755720118
1103	48-49	-0.002408304394002414
1103	50-51	-0.03788876584882672
1103	52-53	-5.925360241363364E-4
1103	54-55	-0.009124677230431644
1103	56-57	0.034342266478731176
1103	58-59	0.018948352670342672
1103	60-61	-0.04079527056707377
1103	62-63	0.0152515742920869
1103	64-65	-0.044281559676065285
1103	66-67	-0.00995445795952321
1103	68-69	-0.005278883180530158
1103	70-71	-0.07907615786913169
1103	72-73	-0.03675969364482512
1103	74-75	-0.035058129811815775
1103	76-77	-0.1033908541219617
1103	78-79	-0.0618102355439234
1103	80-81	-0.059537085619133734
1103	82-83	-0.013881234875263715
1103	84-85	-0.06591195184820009
1103	86-87	0.029063691962214477
1103	88-89	0.04563833147029328
1103	90-91	0.007978989096400824
1103	92-93	-0.03894916935945503
1103	94-95	-0.08039681366264517
1103	96-97	-0.0061010962055974005
1103	98-99	-9.541539789665876E-4
1103	100	0.11022887274137005
1104	1	0.01525057153501308
1104	2	0.010046622437371866
1104	3	0.01611055923964244
1104	4	0.02707123594770877
1104	5	0.027837518341058853
1104	6	-0.007918086858389017
1104	7	0.014740751612656311
1104	8	0.03272051964012945
1104	9	0.030433563943105923
1104	10-11	0.049531277700083365
1104	12-13	0.023440994805660864
1104	14-15	-0.054227971266854524
1104	16-17	-0.017705827602313207
1104	18-19	0.019382570176006197
1104	20-21	-0.02684375583158527
1104	22-23	-0.039743492524621615
1104	24-25	-0.04817359094024454
1104	26-27	-0.08512879127306405
1104	28-29	-0.04781554145782252
1104	30-31	-0.09535264025265633
1104	32-33	-0.05296668087267875
1104	34-35	-0.04087835852831745
1104	36-37	-0.012516520014266064
1104	38-39	-0.05594207198908663
1104	40-41	-0.03749284243234996
1104	42-43	-0.07419665589532798
1104	44-45	-0.05646482957018861
1104	46-47	-0.07381228684122476
1104	48-49	-0.032247345138067374
1104	50-51	-0.020796783567270438
1104	52-53	-0.10153360079667806
1104	54-55	-0.036372129043961365
1104	56-57	-0.06872259487344223
1104	58-59	-0.03258650523896023
1104	60-61	-0.10632988061085058
1104	62-63	-0.031490403001342315
1104	64-65	-0.06766907949815248
1104	66-67	-0.0572269829529759
1104	68-69	-0.11838639180931665
1104	70-71	-0.11553380414385828
1104	72-73	-0.08884325477298916
1104	74-75	-0.07807694483265948
1104	76-77	-0.08893591454178562
1104	78-79	-0.012570853394421988
1104	80-81	0.019584990344519326
1104	82-83	0.05792555000920174
1104	84-85	0.0549604568459614
1104	86-87	0.061224903390503016
1104	88-89	0.08632344760936661
1104	90-91	0.04175211543108048
1104	92-93	0.04847307888856278
1104	94-95	0.03600537399940862
1104	96-97	-0.01734857935502987
1104	98-99	-0.07504815367652284
1104	100	-0.0847451747055743
1105	1	-0.0029839013294790107
1105	2	0.010150623611835385
1105	3	0.003053506128544825
1105	4	-0.0027614755104394817
1105	5	-0.0028553649552307547
1105	6	0.0125966717149808
1105	7	0.02293998326855018
1105	8	0.0355129984147311
1105	9	-0.008761215503184872
1105	10-11	0.010260241627449318
1105	12-13	0.05238317349581223
1105	14-15	0.012486074923913293
1105	16-17	-0.011362173854273294
1105	18-19	-0.05251240244646738
1105	20-21	-0.053720973396806926
1105	22-23	-0.007782263668254075
1105	24-25	-0.023833773098552058
1105	26-27	-0.074410747105631
1105	28-29	-0.052254305769793064
1105	30-31	-0.06777817512103468
1105	32-33	-0.08480922774588606
1105	34-35	-0.015946202760588335
1105	36-37	-0.05433055196028391
1105	38-39	-0.06499824134457555
1105	40-41	-0.07032568979699505
1105	42-43	-0.09519780710329684
1105	44-45	-0.057518068578112036
1105	46-47	-0.05806862756971043
1105	48-49	-0.0780725991814748
1105	50-51	-0.022965055432592862
1105	52-53	-0.12467358957530195
1105	54-55	-0.06552514449659697
1105	56-57	-0.07327931439586166
1105	58-59	-0.06650028165170596
1105	60-61	-0.04874035674872346
1105	62-63	-0.03598042295852366
1105	64-65	-0.031451215570356794
1105	66-67	-0.10569143940693948
1105	68-69	-0.04716702709587395
1105	70-71	-0.006958086056080504
1105	72-73	-0.027131149065347415
1105	74-75	-0.08917056696608228
1105	76-77	-0.095354462217351
1105	78-79	0.005671468615311426
1105	80-81	-0.002581032159262975
1105	82-83	0.07366695628531517
1105	84-85	0.06016945487350256
1105	86-87	0.07310224087323292
1105	88-89	0.056284423548824236
1105	90-91	0.10596975187902302
1105	92-93	0.013761524074638487
1105	94-95	0.019899400363811992
1105	96-97	-0.003995221011024341
1105	98-99	0.004790172100797463
1105	100	-0.1271949874162459
1106	1	-0.02354684395124451
1106	2	-0.02060767479352421
1106	3	-0.010515410381543688
1106	4	0.023839189325521204
1106	5	0.0233105067988717
1106	6	-0.003694218105316338
1106	7	0.026654392865310683
1106	8	0.019429267625120872
1106	9	0.04329592184807041
1106	10-11	0.03253405287184563
1106	12-13	0.0046613206269014995
1106	14-15	-0.009953945310229528
1106	16-17	0.020495151385411248
1106	18-19	0.03042106578111259
1106	20-21	0.03164540902839974
1106	22-23	0.02895052527041031
1106	24-25	0.020380829204292183
1106	26-27	-0.0074646566301268535
1106	28-29	0.0815017765157009
1106	30-31	0.026984435082759717
1106	32-33	-0.003592650205781922
1106	34-35	0.001615342742141479
1106	36-37	-0.06528709674035582
1106	38-39	0.01235999924896447
1106	40-41	-4.751574464663122E-4
1106	42-43	0.03279636353793336
1106	44-45	0.0630990726272671
1106	46-47	0.02935790323917331
1106	48-49	0.018212389015282326
1106	50-51	-0.018133552806972375
1106	52-53	-0.0597165871183023
1106	54-55	-0.025857201152184928
1106	56-57	0.015840018841117853
1106	58-59	-0.016752606168729756
1106	60-61	0.017757089895781064
1106	62-63	0.0015785932769674105
1106	64-65	0.05164906385345347
1106	66-67	-0.007579356736030718
1106	68-69	0.024465434242465278
1106	70-71	0.03325906960225211
1106	72-73	-0.023324601876453244
1106	74-75	-0.010961408609865941
1106	76-77	-0.03061304968181844
1106	78-79	0.09179181355918331
1106	80-81	0.1331984639143471
1106	82-83	0.08061822573659327
1106	84-85	0.04780736015846543
1106	86-87	0.0236327847861304
1106	88-89	0.038240510651966986
1106	90-91	0.04163603313353548
1106	92-93	0.06784629065940351
1106	94-95	0.10573309502103712
1106	96-97	0.11302554306856649
1106	98-99	0.06913533276948414
1106	100	0.07759978061381645
1107	1	0.07739485920987477
1107	2	0.02859682602160518
1107	3	-0.0056890747807329944
1107	4	0.03968135772993975
1107	5	0.042919586796607234
1107	6	-0.0012331838044232768
1107	7	-0.003854113695631156
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2316	18-19	-0.15804205048590347
2316	20-21	-0.16273669795783263
2316	22-23	-0.1868557406693654
2316	24-25	-0.20007644328976681
2316	26-27	-0.18811234236975594
2316	28-29	-0.2955353286071798
2316	30-31	-0.16884307589588587
2316	32-33	-0.11026334811078442
2316	34-35	-0.1839227487382118
2316	36-37	-0.1332527465564297
2316	38-39	-0.20689958367182015
2316	40-41	-0.18139750809491062
2316	42-43	-0.2549886459263817
2316	44-45	-0.2775846557126158
2316	46-47	-0.23704940429897192
2316	48-49	-0.20335909082273673
2316	50-51	-0.17770201371644845
2316	52-53	-0.18480469644045883
2316	54-55	-0.22785944489464782
2316	56-57	-0.36699109473958913
2316	58-59	-0.3356184835464049
2316	60-61	-0.2388710641320344
2316	62-63	-0.24552768882973197
2316	64-65	-0.20385884307846425
2316	66-67	-0.24539065519336134
2316	68-69	-0.20014535196269634
2316	70-71	-0.21925960845745607
2316	72-73	-0.1847740986889832
2316	74-75	-0.19821441743832935
2316	76-77	-0.19662799835543154
2316	78-79	-0.1575913112183045
2316	80-81	-0.21404802164366998
2316	82-83	-0.18468816360361728
2316	84-85	-0.09930700540332538
2316	86-87	-0.19035352795174987
2316	88-89	-0.16047026285110633
2316	90-91	-0.20319538571686735
2316	92-93	-0.1859136579286016
2316	94-95	-0.19015974001955982
2316	96-97	-0.16372854469305764
2316	98-99	-0.1302468175270164
2316	100	-0.3165562569353746
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	9.0
13	45.0
14	248.0
15	910.0
16	2449.0
17	5570.0
18	10140.0
19	16053.0
20	23566.0
21	34872.0
22	43899.0
23	45042.0
24	41839.0
25	42643.0
26	49133.0
27	60478.0
28	74853.0
29	92455.0
30	115177.0
31	146050.0
32	186344.0
33	245218.0
34	369075.0
35	544124.0
36	930011.0
37	1741737.0
38	2025921.0
39	526885.0
40	225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61171903878692	20.981333490991645	13.764112972666442	23.642834497554986
2	15.161288091113079	21.116918386344594	40.74839475552092	22.97339876702141
3	17.890359391756636	27.374097222231637	30.23521612631641	24.500327259695315
4	12.217092045281817	17.675200454081903	39.5409498810896	30.566757619546674
5	12.633754314737333	38.84444028593377	35.32350613979241	13.198299259536494
6	29.56647959245936	38.562136523945185	18.447681821078593	13.423702062516865
7	26.06595170220336	33.04058407747781	23.616444508083624	17.277019712235212
8	25.406042519100346	35.967853436055236	20.516595488708756	18.109508556135665
9	26.37617303730831	15.576636448741077	21.281047318516173	36.766143195434445
10-11	24.67961482049169	26.53222373952044	28.637064842871496	20.151096597116375
12-13	25.22642822392082	24.29529825501521	28.66801112520685	21.81026239585712
14-15	22.659148822068374	25.680770478504954	26.38089546329288	25.279185236133788
16-17	21.661471386364923	28.306433537361475	27.358564375170424	22.67353070110318
18-19	21.238633983785927	28.09081029745459	29.414226556990915	21.256329161768573
20-21	22.4759766379639	27.222526668462383	29.037405169779646	21.264091523794075
22-23	22.570323889016723	26.934770525900152	28.670123193164436	21.82478239191869
24-25	22.111986906905603	27.01655484563817	29.0716255722127	21.79983267524353
26-27	21.96763677602231	27.30112857646792	28.67558218188739	22.055652465622387
28-29	21.523624315241538	27.686950475423274	28.884062945744276	21.905362263590913
30-31	22.458806863830002	27.197205685877908	28.83197490709281	21.512012543199283
32-33	22.36769258117893	27.443822254849625	28.38262088278122	21.805864281190225
34-35	22.38691129346368	27.50294834206395	28.810334231310698	21.29980613316167
36-37	22.220134746377447	27.225965855147816	28.637385038068487	21.916514360406254
38-39	22.20149878581454	27.313057541231387	28.92174310631611	21.563700566637962
40-41	22.148959806202484	26.76333264743042	29.167868691741333	21.91983885462576
42-43	21.92011321991228	27.001557831467682	28.624843035445156	22.45348591317488
44-45	21.935530893142023	27.494384513529035	28.647028792377178	21.92305580095177
46-47	21.930614590998786	27.161097232781824	28.19507683387511	22.71321134234428
48-49	22.27046365315856	27.16576726465052	28.367774003910768	22.19599507828015
50-51	22.235616023002596	27.146258451281874	28.115663315873558	22.50246220984198
52-53	21.87881440309115	27.49309369087601	28.027678798741217	22.600413107291615
54-55	21.704599879478668	27.389320518946853	28.106855016958143	22.799224584616336
56-57	22.293692481613167	27.125355624298024	27.746695563633917	22.834256330454892
58-59	21.7583855404418	27.397912646791074	28.2810168397291	22.56268497303803
60-61	22.126960574790953	27.734030902716	27.765714973811995	22.373293548681055
62-63	22.137064859337578	27.259352330049996	27.76903318135906	22.83454962925337
64-65	21.536066976879408	27.137524061917436	27.973114654867082	23.353294306336075
66-67	21.91398702277567	27.084102998505905	27.73177094439027	23.270139034328157
68-69	21.525336613688363	27.126917074422852	28.011671891545976	23.33607442034281
70-71	21.578656761165146	26.732494372356946	28.055710852574535	23.633138013903373
72-73	21.696382866057764	27.03649136176967	27.923780440261254	23.343345331911316
74-75	21.744818443207688	27.153102140302792	27.788774151878513	23.313305264611
76-77	21.449386927336	26.972302165687456	27.698356370792077	23.879954536184467
78-79	21.305930575470526	26.96046981347791	27.854648489072147	23.878951121979416
80-81	21.214258914567438	27.321649327691244	27.770596426132144	23.693495331609178
82-83	21.660060104852967	26.74161523741078	28.31720310728191	23.281121550454344
84-85	21.67698173606937	26.95554556056977	27.580260044700815	23.787212658660042
86-87	20.78875240569206	27.411227878243675	27.970972909124264	23.82904680694
88-89	21.24639725296301	26.560666083790917	27.97368326850106	24.219253394745007
90-91	21.25833270733224	26.485778565921443	27.974702956831415	24.281185769914902
92-93	21.385091788602313	26.553050269747136	27.86147726174879	24.200380679901762
94-95	20.660422617104064	26.484526783612537	28.179913294013893	24.675137305269505
96-97	20.68919601482069	26.398226398592513	28.08911397897577	24.82346360761103
98-99	20.282307215167826	26.62034135229574	27.9787104144905	25.118641018045935
100	20.28623589586718	26.547227471963943	27.695734461691078	25.470802170477803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	667.0
1	461.0
2	328.0
3	408.5
4	391.0
5	427.0
6	656.0
7	1227.0
8	1974.0
9	2490.5
10	2504.5
11	2474.5
12	2840.5
13	3237.5
14	3707.0
15	4337.0
16	4988.0
17	5740.0
18	6609.0
19	7660.0
20	8730.0
21	9898.0
22	11530.0
23	13639.0
24	16303.0
25	20005.5
26	25347.0
27	31959.0
28	39994.5
29	52584.5
30	70352.0
31	88364.5
32	111385.5
33	136994.0
34	161253.5
35	184748.0
36	210019.0
37	240927.5
38	256300.5
39	257031.5
40	250824.5
41	242291.0
42	242509.0
43	250361.5
44	262215.5
45	286084.5
46	306328.0
47	315894.0
48	323778.5
49	319546.5
50	308606.0
51	302689.0
52	292637.0
53	273944.0
54	256487.5
55	227259.5
56	197301.5
57	165664.0
58	133638.5
59	114936.5
60	89691.0
61	63426.0
62	46825.0
63	35760.5
64	25026.5
65	14020.0
66	8365.0
67	5733.0
68	4209.0
69	3006.5
70	2077.0
71	1422.5
72	910.0
73	529.0
74	345.5
75	222.5
76	118.0
77	50.0
78	28.0
79	18.0
80	10.5
81	7.5
82	4.0
83	1.5
84	2.0
85	2.0
86	0.5
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00505764700634077
2	0.03278656960142623
3	0.0012745812830992826
4	0.0012203437816908026
5	0.007267825188736336
6	1.3559375352120028E-5
7	8.000031457750818E-4
8	0.0067390095500036535
9	0.001342378159859883
10-11	2.7118750704240057E-4
12-13	9.49156274648402E-5
14-15	9.966140883808222E-4
16-17	1.0847500281696023E-4
18-19	0.0010915297158456622
20-21	1.966109426057404E-4
22-23	0.0017627187957756038
24-25	0.0012339031570429225
26-27	0.002027126615141944
28-29	2.2372969330998046E-4
30-31	0.001871193798592564
32-33	0.0016271250422544034
34-35	8.610203348596219E-4
36-37	0.004088151668664189
38-39	9.220375239441618E-4
40-41	0.0035728954052836274
42-43	0.004020354791903589
44-45	0.003742387597185128
46-47	0.0022237375577476848
48-49	0.00975597056585036
50-51	0.006752568925355774
52-53	0.0050644266940168305
54-55	0.00465086574577717
56-57	0.0035796750929596873
58-59	0.005647479834157992
60-61	0.008176303337328378
62-63	0.0034983188408469674
64-65	0.003349165711973647
66-67	0.003816964161621788
68-69	0.0021627203686631446
70-71	0.0029017063253536863
72-73	0.0065898564211303344
74-75	0.007735623638384476
76-77	0.004271203235917809
78-79	0.002908486013029746
80-81	0.0021695000563392046
82-83	0.00486103606373503
84-85	0.0021966188070434447
86-87	0.003715268846480888
88-89	5.830531401411612E-4
90-91	0.0015864469161980433
92-93	1.1525469049302023E-4
94-95	0.0022101781823955645
96-97	0.001979668801409524
98-99	0.0013220390968317027
100	6.779687676060014E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7374971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.944772314673365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62506052096697	44.134374895834114
2	15.868715608897762	19.024930882033594
3	5.148856630189806	9.25941115132873
4	2.1933147320887114	5.259110089179064
5	1.0798590643033874	3.236595287080139
6	0.5996813698645518	2.156865790672919
7	0.35413372526297543	1.4859925870885307
8	0.23413517478919257	1.122814379487552
9	0.163275155919862	0.8808742841633028
>10	0.6684433837287881	6.66096179614909
>50	0.034867513556617866	1.4604026855605805
>100	0.02698941798129792	3.187131779935958
>500	0.0019563211374769673	0.8132383353484538
>1k	6.224586486355962E-4	0.5956065864802806
>5k	2.964088801024552E-5	0.1549739162014196
>10k+	5.928177602049104E-5	0.5667155534561199
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17222	0.23351956231421112	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14673	0.19895671454165717	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8722	0.11826487182119089	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.3559375352120028E-5	0.0
12-13	0.0	0.0	0.0	1.3559375352120028E-5	1.3559375352120028E-5
14-15	0.0	0.0	0.0	1.3559375352120028E-5	2.7118750704240057E-5
16-17	0.0	0.0	0.0	2.7118750704240057E-5	4.74578137324201E-5
18-19	0.0	0.0	0.0	5.4237501408480114E-5	5.4237501408480114E-5
20-21	0.0	0.0	0.0	6.779687676060014E-5	6.101718908454013E-5
22-23	0.0	0.0	0.0	1.491531288733203E-4	6.779687676060014E-5
24-25	0.0	0.0	0.0	5.491547017608612E-4	6.779687676060014E-5
26-27	0.0	0.0	0.0	0.0012339031570429227	6.779687676060014E-5
28-29	0.0	0.0	0.0	0.004339000112678409	6.779687676060014E-5
30-31	0.0	0.0	0.0	0.0189560067422638	6.779687676060014E-5
32-33	0.0	0.0	0.0	0.05452902797855069	6.779687676060014E-5
34-35	0.0	0.0	0.0	0.11223772947717353	6.779687676060014E-5
36-37	0.0	0.0	0.0	0.20568894440398477	6.779687676060014E-5
38-39	0.0	0.0	0.0	0.3427538901508901	6.779687676060014E-5
40-41	0.0	0.0	0.0	0.49068667524251963	6.779687676060014E-5
42-43	0.0	0.0	0.0	0.6407279432014037	6.779687676060014E-5
44-45	0.0	0.0	0.0	0.8203557681786138	6.779687676060014E-5
46-47	0.0	0.0	0.0	1.0346549159311949	6.779687676060014E-5
48-49	6.779687676060014E-6	0.0	0.0	1.26589650318625	6.779687676060014E-5
50-51	1.3559375352120028E-5	0.0	0.0	1.5080601672874376	6.779687676060014E-5
52-53	1.3559375352120028E-5	0.0	0.0	1.7471255141206656	7.457656443666016E-5
54-55	1.3559375352120028E-5	0.0	0.0	2.014062156990177	8.135625211272017E-5
56-57	1.3559375352120028E-5	0.0	0.0	2.3216294681023153	8.813593978878019E-5
58-59	1.3559375352120028E-5	0.0	0.0	2.665691837974685	9.49156274648402E-5
60-61	1.3559375352120028E-5	0.0	0.0	3.023483075391076	9.49156274648402E-5
62-63	1.3559375352120028E-5	0.0	0.0	3.3897285833395143	1.0847500281696023E-4
64-65	1.3559375352120028E-5	0.0	0.0	3.762530049270702	1.0847500281696023E-4
66-67	1.3559375352120028E-5	0.0	0.0	4.17929778978114	1.0847500281696023E-4
68-69	1.3559375352120028E-5	0.0	0.0	4.639977567369417	1.0847500281696023E-4
70-71	1.3559375352120028E-5	0.0	0.0	5.142006660093985	1.2881406584514026E-4
72-73	1.3559375352120028E-5	0.0	0.0	5.644822196588977	1.3559375352120028E-4
74-75	1.3559375352120028E-5	0.0	0.0	6.1348376827515665	1.3559375352120028E-4
76-77	1.3559375352120028E-5	0.0	0.0	6.671605895128265	1.3559375352120028E-4
78-79	1.3559375352120028E-5	0.0	0.0	7.255581072793371	1.3559375352120028E-4
80-81	1.3559375352120028E-5	0.0	0.0	7.866946188669759	1.3559375352120028E-4
82-83	1.3559375352120028E-5	0.0	0.0	8.496385951890524	1.491531288733203E-4
84-85	1.3559375352120028E-5	0.0	0.0	9.116659577373253	1.5593281654938031E-4
86-87	1.3559375352120028E-5	0.0	0.0	9.749523082870429	1.6271250422544034E-4
88	1.3559375352120028E-5	0.0	0.0	10.242860615994287	1.6271250422544034E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11910	0.0	51.359783	1
GTATCAA	21930	0.0	40.69258	1
CAACGCA	25110	0.0	34.385303	5
ATCAACG	25315	0.0	34.126114	3
TATCAAC	27365	0.0	32.405052	2
AACGCAG	27990	0.0	31.467297	6
TCAACGC	28260	0.0	30.702438	4
GTACTAG	4975	0.0	29.675236	1
ACGCAGA	30340	0.0	28.332888	7
CGCAGAG	30730	0.0	27.881922	8
GTACATG	29505	0.0	25.65594	1
TAGGCAT	6115	0.0	25.441454	5
GTGGTAT	4485	0.0	25.369423	1
GCATCGT	5010	0.0	25.329386	8
TACATGG	29860	0.0	25.099495	2
GCAGAGT	33700	0.0	24.962402	9
ACATGGG	29085	0.0	24.886904	3
CGACGAG	2615	0.0	23.274508	24-25
CATGGGG	21925	0.0	23.023853	4
CTAGGCA	6970	0.0	22.927647	4
>>END_MODULE