FastQCFastQC Report
Thu 26 May 2016
ERR580128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12567872
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337330.268406616490047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262610.20895343300759267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161880.12880462181664487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182000.054.954361
GTATCAA340900.043.2982521
CAACGCA417550.034.4111375
ATCAACG420000.034.191013
TATCAAC440700.033.269222
AACGCAG465150.031.540686
TCAACGC475550.030.374344
ACGCAGA497250.028.7104957
CGCAGAG511900.027.824248
GTACATG466100.026.1796781
GTGGTAT65300.025.9956111
TACATGG473600.025.2589282
GCAGAGT553500.025.1083649
ACATGGG483100.024.079913
GTACTAG54700.022.95251
CATGGGG369050.022.0670624
GAGTACT292600.021.73092512-13
CAGAGTA543500.021.00673310-11
AGAGTAC508450.020.98958210-11
ATGGGGG249050.019.8790665
Produced by FastQC (version 0.11.5)