FastQCFastQC Report
Thu 26 May 2016
ERR580128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12567872
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306480.2438598992733217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254510.2025084278388577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156530.1245477356866779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA184250.053.686221
GTATCAA345400.043.7701071
CAACGCA423850.034.9065
ATCAACG426900.034.6032183
TATCAAC450550.033.4116862
AACGCAG467650.032.1977046
TCAACGC484350.030.750284
ACGCAGA503850.029.119577
CGCAGAG518900.028.1753738
GCAGAGT570450.025.2503819
GTACATG457900.025.1357061
GTGGTAT62350.025.041681
TACATGG467600.024.3033542
ACATGGG476650.023.2904853
GAGTACT307350.021.85747312-13
CATGGGG366650.021.5733994
AGAGTAC521450.021.45338810-11
CAGAGTA555800.021.015310-11
GTACTAG54700.020.7826841
TAGTACT71600.020.4798954
Produced by FastQC (version 0.11.5)