##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580128_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12567872
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52946704103925	34.0	31.0	34.0	31.0	34.0
2	32.73291866753576	34.0	31.0	34.0	31.0	34.0
3	32.811646076599125	34.0	31.0	34.0	31.0	34.0
4	36.28561724689749	37.0	37.0	37.0	35.0	37.0
5	36.217623317614944	37.0	37.0	37.0	35.0	37.0
6	36.24550162509612	37.0	37.0	37.0	35.0	37.0
7	36.20972691319581	37.0	37.0	37.0	35.0	37.0
8	36.202913667484836	37.0	37.0	37.0	35.0	37.0
9	37.996213758383284	39.0	38.0	39.0	35.0	39.0
10-11	37.946328662481605	39.0	38.0	39.0	35.0	39.0
12-13	37.9087239669532	39.0	38.0	39.0	35.0	39.0
14-15	39.31012795961003	41.0	39.0	41.0	36.0	41.0
16-17	39.29959073421499	41.0	39.0	41.0	36.0	41.0
18-19	39.305382685310605	41.0	39.0	41.0	36.0	41.0
20-21	39.28997363276774	41.0	39.0	41.0	36.0	41.0
22-23	39.21806607355644	41.0	39.0	41.0	36.0	41.0
24-25	39.13763547241729	41.0	39.0	41.0	35.0	41.0
26-27	38.99691976493713	40.0	38.5	41.0	35.0	41.0
28-29	38.88914213161942	40.0	38.0	41.0	35.0	41.0
30-31	38.76603931039399	40.0	38.0	41.0	35.0	41.0
32-33	38.6114043809485	40.0	38.0	41.0	35.0	41.0
34-35	38.572911269306374	40.0	38.0	41.0	34.5	41.0
36-37	38.602412882626425	40.0	38.0	41.0	35.0	41.0
38-39	38.50238660132757	40.0	38.0	41.0	34.0	41.0
40-41	38.36340229276682	40.0	38.0	41.0	34.0	41.0
42-43	38.31633004378148	40.0	38.0	41.0	34.0	41.0
44-45	38.19658690031216	40.0	38.0	41.0	34.0	41.0
46-47	37.97202119817898	40.0	37.0	41.0	33.0	41.0
48-49	37.7348464401929	40.0	37.0	41.0	33.0	41.0
50-51	37.54587693923044	40.0	36.5	41.0	33.0	41.0
52-53	37.35806805639014	40.0	36.0	41.0	32.5	41.0
54-55	37.14855160046187	39.0	35.5	41.0	32.0	41.0
56-57	36.91588166238485	39.0	35.0	41.0	32.0	41.0
58-59	36.672653174698155	39.0	35.0	41.0	31.0	41.0
60-61	36.40383371982146	38.0	35.0	40.0	31.0	41.0
62-63	36.11113412835522	37.5	35.0	40.0	31.0	41.0
64-65	35.816794481993455	37.0	35.0	40.0	30.5	41.0
66-67	35.492045908806205	36.5	35.0	39.5	30.0	41.0
68-69	35.14784810029892	36.0	34.5	39.0	30.0	41.0
70-71	34.83738826270668	35.5	34.0	39.0	29.0	40.5
72-73	34.50105153044207	35.0	34.0	38.0	29.0	40.0
74-75	34.15957295714024	35.0	34.0	37.0	29.0	39.5
76-77	32.92467368381855	34.5	32.5	36.0	27.0	39.0
78-79	33.55773610679676	35.0	33.5	36.0	29.0	39.0
80-81	33.505324051677164	35.0	34.0	36.0	29.0	37.5
82-83	33.305637939342475	35.0	34.0	35.5	29.0	37.0
84-85	33.095578750324634	35.0	34.0	35.0	29.0	37.0
86-87	32.92662656016866	35.0	33.0	35.0	29.0	36.0
88-89	32.768262518905345	35.0	33.0	35.0	28.0	36.0
90-91	32.60911501167421	35.0	33.0	35.0	27.0	36.0
92-93	32.49281644497971	35.0	33.0	35.0	27.0	35.5
94-95	32.399466234220085	35.0	33.0	35.0	27.0	35.0
96-97	32.30182607684101	35.0	33.0	35.0	27.0	35.0
98-99	32.226793247098634	35.0	33.0	35.0	26.5	35.0
100	30.95536483821605	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1777297460797982
1101	2	0.11399157248103364
1101	3	0.06535443485907422
1101	4	0.055711192535611076
1101	5	0.026418655871964347
1101	6	0.05475005913561404
1101	7	0.027029025975160437
1101	8	0.052261565036445745
1101	9	0.011504337054326186
1101	10-11	0.03875359149255786
1101	12-13	0.003558895797873163
1101	14-15	0.04910720717205663
1101	16-17	0.01705418520312918
1101	18-19	0.020798228414015796
1101	20-21	0.011690265120755328
1101	22-23	0.016197436379741248
1101	24-25	-0.00459444020390265
1101	26-27	0.017403788658342023
1101	28-29	-9.563608001172952E-5
1101	30-31	-0.0011145214933279135
1101	32-33	0.01065580173560221
1101	34-35	-0.005950330407209492
1101	36-37	0.06323783759575718
1101	38-39	0.08285415351709702
1101	40-41	0.12440574565774654
1101	42-43	0.07229792838581517
1101	44-45	0.03165770538988255
1101	46-47	0.06535066453997018
1101	48-49	0.07691622464689374
1101	50-51	0.07829292411550881
1101	52-53	0.10698156400535908
1101	54-55	0.0811785600798558
1101	56-57	0.06232952848674245
1101	58-59	0.06671370397840093
1101	60-61	0.027913548472717764
1101	62-63	0.022779407637891325
1101	64-65	-0.0015088575505259882
1101	66-67	-0.006500294952083152
1101	68-69	-0.020514149602441023
1101	70-71	-0.07128792588729027
1101	72-73	-0.028560703513427654
1101	74-75	-0.007210145993241213
1101	76-77	-0.025793190917539732
1101	78-79	0.01132777617962688
1101	80-81	0.039817261432240514
1101	82-83	0.08920902981864742
1101	84-85	0.020323974695827474
1101	86-87	0.05049353402108636
1101	88-89	0.07454307578841934
1101	90-91	0.05472362449637558
1101	92-93	-0.05010740026737892
1101	94-95	-0.023287461119508635
1101	96-97	-0.006617297925032517
1101	98-99	-0.027696046277405628
1101	100	-0.03854086943232815
1102	1	0.11673710661670356
1102	2	0.0910001168379253
1102	3	0.05215148708379047
1102	4	0.043689670248873824
1102	5	0.029210226461486855
1102	6	0.04964002691449565
1102	7	0.03925936428001364
1102	8	0.023758503902413963
1102	9	0.054942974946108336
1102	10-11	0.036557242855224104
1102	12-13	0.013317179609735774
1102	14-15	0.08433273601951186
1102	16-17	0.028564377626707937
1102	18-19	0.017424111106109308
1102	20-21	0.010532452785945168
1102	22-23	-0.0057024330731536566
1102	24-25	-0.024533741190005287
1102	26-27	-0.02916559294276766
1102	28-29	0.003170383866901716
1102	30-31	-0.005164915880499166
1102	32-33	-0.05174537633758547
1102	34-35	0.003103482389015255
1102	36-37	0.04087298617005786
1102	38-39	0.04899410132012605
1102	40-41	0.0692857736588266
1102	42-43	0.06383376121973328
1102	44-45	0.028466019052309832
1102	46-47	0.03986378850828487
1102	48-49	0.07428595096230595
1102	50-51	0.08822710607699946
1102	52-53	0.04254553591038501
1102	54-55	-0.030476793702057137
1102	56-57	0.013307092229766226
1102	58-59	0.033432906062280665
1102	60-61	0.07008377805998123
1102	62-63	0.056836043081155196
1102	64-65	-0.02204324384614864
1102	66-67	-0.03682930550146324
1102	68-69	0.023711628116842576
1102	70-71	-0.045646018992847814
1102	72-73	-0.09764441060131901
1102	74-75	-0.11694100497172144
1102	76-77	-0.0750209880852779
1102	78-79	0.0015968404209942832
1102	80-81	-0.014253047647216022
1102	82-83	0.027450957191277325
1102	84-85	0.013339998950229415
1102	86-87	-0.02380039689477087
1102	88-89	0.013509027091259895
1102	90-91	-0.022087817372117513
1102	92-93	-0.0246383983975349
1102	94-95	-0.10158550360011986
1102	96-97	-0.11840448585076047
1102	98-99	-0.18500619326795942
1102	100	-0.04631542059241056
1103	1	0.0837051174685115
1103	2	0.0575645204459434
1103	3	0.03505954223952301
1103	4	-0.01648167078693774
1103	5	-0.01237855492301776
1103	6	-0.0036737541089166825
1103	7	0.009068157663790544
1103	8	-0.019541977914499853
1103	9	-0.014475372440458045
1103	10-11	-0.030447026194593718
1103	12-13	0.004076695589340318
1103	14-15	-0.015622265082548381
1103	16-17	-0.013766579535960943
1103	18-19	-0.0142001201478692
1103	20-21	-0.038730420250708164
1103	22-23	-0.08008762895065047
1103	24-25	-0.07172946622021215
1103	26-27	-0.09579502230621983
1103	28-29	-0.09311362689604863
1103	30-31	-0.1201212800654119
1103	32-33	-0.15104007731011393
1103	34-35	-0.13353020948250105
1103	36-37	-0.1021995346950888
1103	38-39	-0.15571271863448288
1103	40-41	-0.14850906724910118
1103	42-43	-0.16737158680617625
1103	44-45	-0.07356293413568693
1103	46-47	-0.10396918944332612
1103	48-49	-0.17280913133661358
1103	50-51	-0.20018403092840487
1103	52-53	-0.17807902158966016
1103	54-55	-0.059583862062233095
1103	56-57	-0.1051242620566768
1103	58-59	-0.11330839324940456
1103	60-61	-0.1587730258284452
1103	62-63	-0.18717605282987648
1103	64-65	-0.1647235666879041
1103	66-67	-0.1743785913581064
1103	68-69	-0.14123686328498053
1103	70-71	-0.10623962748474725
1103	72-73	-0.16761918660590425
1103	74-75	-0.20941450776171422
1103	76-77	-0.24976775877070168
1103	78-79	-0.14057014522671096
1103	80-81	-0.10646811200227546
1103	82-83	-0.09851351379040807
1103	84-85	-0.11678223742276828
1103	86-87	-0.09892550484192952
1103	88-89	-0.12168807132021442
1103	90-91	-0.1160482009835917
1103	92-93	-0.17244032772487827
1103	94-95	-0.16862621544220247
1103	96-97	-0.15068191845167433
1103	98-99	-0.1450015771161688
1103	100	-0.0702078113386797
1104	1	0.08925455064848364
1104	2	0.042142462765191624
1104	3	0.025350836473336358
1104	4	-0.009717708726917351
1104	5	-0.033163425280491765
1104	6	-0.011433161961143412
1104	7	-0.008962777195669958
1104	8	-0.005987038374883014
1104	9	-0.02478657244146376
1104	10-11	-0.018871013368247702
1104	12-13	-0.041287011304056875
1104	14-15	-0.01857851710555991
1104	16-17	-0.03666008525579656
1104	18-19	-0.05863689587959442
1104	20-21	-0.07297684117991565
1104	22-23	-0.11390356641089738
1104	24-25	-0.08816946679956317
1104	26-27	-0.049598938219624245
1104	28-29	-0.07613714353539791
1104	30-31	-0.070287717720241
1104	32-33	-0.12252703371138551
1104	34-35	-0.14265515548314767
1104	36-37	-0.07493921310454255
1104	38-39	6.518528782990529E-4
1104	40-41	-0.0349003959921248
1104	42-43	-0.05836822593685298
1104	44-45	-0.08771156743254238
1104	46-47	-0.06903521349145336
1104	48-49	-0.021154122022899458
1104	50-51	-0.07963302657193339
1104	52-53	-0.10696536916790933
1104	54-55	-0.15191108932298647
1104	56-57	-0.06723382941952849
1104	58-59	-0.08948164332402087
1104	60-61	-0.13830484671809984
1104	62-63	-0.10410381082713371
1104	64-65	-0.1185405967298081
1104	66-67	-0.11947641047696322
1104	68-69	-0.09545589725387771
1104	70-71	-0.09695310849398453
1104	72-73	-0.09253022149168544
1104	74-75	-0.1277666836679927
1104	76-77	-0.18090403739350336
1104	78-79	-0.0816780576589693
1104	80-81	-0.10073571075339771
1104	82-83	-0.03788293044626556
1104	84-85	-0.06668957264446362
1104	86-87	-0.10123873950076501
1104	88-89	-0.03054264271963092
1104	90-91	-0.043936520876386
1104	92-93	-0.08831160334386823
1104	94-95	-0.09929743841577476
1104	96-97	-0.11392071775796353
1104	98-99	-0.07606491725479003
1104	100	-0.07020145106507414
1105	1	0.08810437898107182
1105	2	0.06689640613982561
1105	3	0.010271968980674728
1105	4	0.035052157422263974
1105	5	-0.02314028749873387
1105	6	0.005262613527733606
1105	7	0.018196002721730054
1105	8	-0.003554585770018548
1105	9	-0.0032719185130218875
1105	10-11	-0.04041552249896796
1105	12-13	-0.03446584396565555
1105	14-15	0.003237176779101958
1105	16-17	-5.003350443288923E-4
1105	18-19	-0.026861257724640097
1105	20-21	-0.034394963287695646
1105	22-23	-0.05553639718166181
1105	24-25	-0.0347037754028392
1105	26-27	-0.021629838353973696
1105	28-29	-0.03305036441414444
1105	30-31	-0.03400618316284465
1105	32-33	-0.029606946136119916
1105	34-35	-0.009234371286503062
1105	36-37	-0.02385285693197403
1105	38-39	0.028214051216359337
1105	40-41	0.033107266843124705
1105	42-43	0.07729630110699048
1105	44-45	0.039694015490290724
1105	46-47	0.025499169749828354
1105	48-49	0.02121600784722233
1105	50-51	-0.01064175921859345
1105	52-53	0.013461311431186118
1105	54-55	0.010867186041494392
1105	56-57	-0.01657054634245725
1105	58-59	-0.00690576135158949
1105	60-61	0.035906229561945224
1105	62-63	-0.0069322180520927645
1105	64-65	-0.01763722806781942
1105	66-67	-0.01000770868067491
1105	68-69	-0.009879318660061642
1105	70-71	-0.0753396836046889
1105	72-73	-0.07381698070948062
1105	74-75	-0.11437090320324472
1105	76-77	-0.11710558488815082
1105	78-79	-0.029814471054002922
1105	80-81	-0.029934969349213247
1105	82-83	-0.0036841694371645417
1105	84-85	0.03887618343853916
1105	86-87	0.01828283088286753
1105	88-89	0.027607703103122105
1105	90-91	0.016515412247962047
1105	92-93	0.013575618629559472
1105	94-95	-0.004308136529914464
1105	96-97	-0.031799630033425785
1105	98-99	-0.055932434794719654
1105	100	-2.9534858676072417E-5
1106	1	0.10204306556681786
1106	2	0.06943453250148224
1106	3	0.01095201877836871
1106	4	0.024645994957275263
1106	5	0.010655230708550789
1106	6	0.03848234586511268
1106	7	0.02445495074538684
1106	8	0.018662967918125162
1106	9	0.031533559233636765
1106	10-11	0.004945510803246123
1106	12-13	-0.026222768957872233
1106	14-15	0.04845737596988897
1106	16-17	0.002655593993956984
1106	18-19	0.015659750678722162
1106	20-21	-0.02042670931944457
1106	22-23	-0.009168516696135498
1106	24-25	-0.004673429931322914
1106	26-27	-0.02727253987902145
1106	28-29	-0.02884401520778823
1106	30-31	-0.02184465188366147
1106	32-33	-0.018008751302637904
1106	34-35	-0.0023615110312888987
1106	36-37	0.04329855919003478
1106	38-39	0.0064913621407285405
1106	40-41	-0.03602205155089422
1106	42-43	0.013738100048790614
1106	44-45	-0.012155502094522319
1106	46-47	-0.02333525290812588
1106	48-49	-0.006468399248944934
1106	50-51	0.0056452000095461585
1106	52-53	-0.01870800073812262
1106	54-55	0.0045234263153801635
1106	56-57	-0.016378358356149647
1106	58-59	-0.05909919428031429
1106	60-61	-0.029859410027221145
1106	62-63	-0.053766951945654284
1106	64-65	-0.034749739739147856
1106	66-67	-0.037534941796145915
1106	68-69	-0.038619592547391335
1106	70-71	-0.039422316657912404
1106	72-73	-0.041746485844868175
1106	74-75	-0.06605079738314146
1106	76-77	-0.0730711933537691
1106	78-79	-0.005167723466072971
1106	80-81	0.02425348380982939
1106	82-83	0.0347938884526684
1106	84-85	0.07305918997448657
1106	86-87	0.05947875369643896
1106	88-89	0.06913353565837355
1106	90-91	0.05703128526383949
1106	92-93	0.011670078635788173
1106	94-95	0.029274185287704313
1106	96-97	0.03839956075400153
1106	98-99	0.042873976658746926
1106	100	0.08532527373142784
1107	1	0.05198572831224624
1107	2	0.02807140376810935
1107	3	0.014643988733318736
1107	4	0.02756819770647212
1107	5	0.00993622652933368
1107	6	0.002272080416091171
1107	7	0.005574433594972561
1107	8	0.007909112008562147
1107	9	-0.0065695013607722785
1107	10-11	0.015758451546979302
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2316	3	-4.279129079591826E-4
2316	4	-0.13020855182211477
2316	5	-0.08599959092972398
2316	6	-0.05049272308967545
2316	7	-0.03366497155303705
2316	8	4.904405889831764E-4
2316	9	0.011887243760483557
2316	10-11	0.013230950004128772
2316	12-13	-0.002952578042219045
2316	14-15	0.013662449731597803
2316	16-17	0.006159993939057529
2316	18-19	0.0057353199263801
2316	20-21	1.606428122613579E-5
2316	22-23	-0.011813152287814432
2316	24-25	-0.021101738701894135
2316	26-27	-0.005316641568910541
2316	28-29	0.019252231155242328
2316	30-31	-0.29708197609344467
2316	32-33	-0.11497712445548558
2316	34-35	-0.1929414118764825
2316	36-37	-0.4019133097882559
2316	38-39	-0.25818456025485403
2316	40-41	-0.21000475170900046
2316	42-43	-0.317530492889496
2316	44-45	-0.20733497941075996
2316	46-47	-0.36235768503080124
2316	48-49	-0.3018796623627864
2316	50-51	-0.32177087511177405
2316	52-53	-0.2811313483792546
2316	54-55	-0.1551444666534607
2316	56-57	-0.9266543045886451
2316	58-59	-0.7589215047330313
2316	60-61	-0.376102239887544
2316	62-63	-0.16866370193421432
2316	64-65	-0.12158423639322535
2316	66-67	-0.08015822865878164
2316	68-69	-0.19056314028308208
2316	70-71	-0.17844089928757967
2316	72-73	-0.07596653563127376
2316	74-75	-0.08023203592009054
2316	76-77	-0.07089991266161633
2316	78-79	-0.15216848828097085
2316	80-81	-0.14456428689594958
2316	82-83	-0.30186453414202674
2316	84-85	-0.12396972443458765
2316	86-87	-0.21920895893650538
2316	88-89	-0.2785495722692133
2316	90-91	-0.18347702574357072
2316	92-93	-0.19539789273505903
2316	94-95	-0.13377937209007484
2316	96-97	-0.28980052307061754
2316	98-99	-0.12922817414308696
2316	100	-0.4703845891610996
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	1.0
9	0.0
10	0.0
11	3.0
12	18.0
13	126.0
14	554.0
15	1717.0
16	4319.0
17	9521.0
18	17993.0
19	27688.0
20	40758.0
21	59415.0
22	77039.0
23	78056.0
24	73094.0
25	73128.0
26	81353.0
27	97803.0
28	120781.0
29	149078.0
30	184565.0
31	229253.0
32	288511.0
33	377777.0
34	553122.0
35	860715.0
36	1577719.0
37	3212529.0
38	3577499.0
39	793635.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.289656866314814	20.085754675640423	13.746251886034338	23.87833657201042
2	15.798083391060121	20.57116976990922	39.51561376346673	24.11513307556393
3	18.198116248465	25.81977390875778	30.60364599997406	25.37846384280316
4	12.98510363987813	17.07466624166186	38.553264435555874	31.38696568290414
5	13.523301718617972	37.83774794308603	34.75750341224241	13.881446926053586
6	30.5854801831209	37.56510250900073	17.80932364683536	14.040093661043015
7	26.898155596602063	32.81327653447389	22.660265575884132	17.628302293039912
8	26.154508160948247	34.44752364387805	20.46431045714216	18.93365773803154
9	26.58666442384118	15.173350968264842	20.97221392258321	37.267770685310765
10-11	25.14621818730982	25.7446254581726	27.99615797818277	21.11299837633481
12-13	25.664753135000456	23.674494012943015	28.07562878042652	22.58512407163001
14-15	23.22553402057131	24.94215694664268	25.734078743574525	26.09823028921149
16-17	22.46275468989046	27.720245873252463	26.678421520931472	23.138577915925605
18-19	22.193558890177638	27.193256130914854	28.693851981831898	21.919332997075607
20-21	23.30991615264296	26.322458962313167	28.259478678830842	22.108146206213032
22-23	23.473046669148662	26.001660745077142	27.89983698886357	22.625455596910626
24-25	23.06855925967419	26.198524300693073	28.181942814185252	22.55097362544749
26-27	22.897246531857103	26.53881149192706	27.876472884053882	22.68746909216195
28-29	22.577198962809003	26.670004392253766	28.045855304383505	22.706941340553726
30-31	23.08841278958438	26.300103772215312	28.004147546640308	22.607335891560005
32-33	23.141018886999504	26.614215511824508	27.495693971655815	22.749071629520177
34-35	23.201392562685854	26.472557293725718	27.90735538184464	22.41869476174379
36-37	23.19445495540554	26.265849302500577	27.75172650991183	22.78796923218205
38-39	23.185695122152104	26.293835739021453	27.895625102108752	22.62484403671769
40-41	23.08922325108714	25.903490367168814	28.15966527757369	22.847621104170354
42-43	22.959275020016587	26.057576789131094	27.761942752324764	23.221205438527555
44-45	22.86741487242247	26.475104594428718	27.71275245399709	22.944728079151727
46-47	22.94784574003135	26.267336605203912	27.374177049114586	23.410640605650155
48-49	23.343220803133537	26.149569065658973	27.421763301302143	23.085446829905347
50-51	23.17717165906891	26.284429966436612	27.063798768139524	23.47459960635496
52-53	22.869665537677406	26.54481741490835	27.040284144193343	23.545232903220903
54-55	22.8208718740521	26.472082785479824	27.036825463817475	23.670219876650602
56-57	23.172228898653756	26.298437961681802	26.84870349454996	23.680629645114475
58-59	22.653954697517175	26.3694056583386	27.33017934420918	23.64646029993504
60-61	22.89660054065056	26.615598152169884	26.951881559111833	23.535919748067723
62-63	23.03821130516493	26.269875948594866	26.81990352516625	23.87200922107395
64-65	22.47881494165257	26.284264096716853	27.07162021734875	24.165300744281826
66-67	22.907757330949973	26.05876536205831	26.91431223353054	24.11916507346118
68-69	22.555850983096576	26.18187958038176	27.003416665861028	24.258852770660635
70-71	22.58013669181554	26.03437581421129	26.971261108930268	24.4142263850429
72-73	22.886792056480797	26.121334283082557	26.8153689412906	24.176504719146042
74-75	22.75272601689999	26.48494812152907	26.64776909445232	24.11455676711862
76-77	22.59203662805756	26.377421291490734	26.524321031811237	24.506221048640473
78-79	22.498259919750424	26.46348258338311	26.50682793764237	24.531429559224097
80-81	22.360854624700295	26.64148937973198	26.666115788360568	24.33154020720716
82-83	22.691395275347713	26.132195848524713	27.018198942690695	24.15820993343688
84-85	22.776431290421144	26.298091612771806	26.416777002597136	24.50870009420991
86-87	22.124973691612787	26.631933627668985	26.711401866035505	24.531690814682726
88-89	22.40230493351542	26.074307760455795	26.661195477181852	24.86219182884693
90-91	22.3552881109028	26.12214244713926	26.655814359101175	24.86675508285676
92-93	22.456798279832455	26.148640955128073	26.608696496303637	24.78586426873584
94-95	21.901412325565936	26.104758529833806	26.869153031556447	25.124676113043815
96-97	22.059758587794533	26.09111437292925	26.651796722580933	25.197330316695275
98-99	21.78238761997813	26.309133889732195	26.540980010269262	25.367498480020412
100	22.06575478323446	26.02823209184421	26.339271434302745	25.566741690618578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1002.0
1	736.0
2	662.0
3	884.5
4	757.5
5	763.0
6	1293.0
7	2567.0
8	4218.0
9	5026.5
10	4506.5
11	4010.5
12	4405.0
13	5168.5
14	6116.0
15	7206.0
16	8392.0
17	9724.5
18	11009.5
19	12296.5
20	13798.0
21	15195.0
22	16988.5
23	19521.0
24	22823.0
25	27225.5
26	33914.5
27	42687.0
28	53339.0
29	70898.0
30	95011.0
31	119335.0
32	149774.5
33	183480.5
34	218227.0
35	249815.5
36	282619.0
37	327162.0
38	349192.5
39	354341.0
40	355337.5
41	352917.0
42	363503.5
43	387465.0
44	417987.5
45	461924.0
46	501182.0
47	531104.5
48	556203.5
49	555759.5
50	538876.5
51	528860.0
52	523017.5
53	510344.5
54	495176.5
55	456025.5
56	411239.5
57	356681.5
58	301958.0
59	269388.5
60	225971.5
61	179787.0
62	142281.5
63	110390.5
64	81477.0
65	56203.5
66	39523.5
67	29215.0
68	22681.5
69	17410.0
70	13463.5
71	10588.5
72	8182.0
73	6354.5
74	4817.0
75	3307.0
76	2183.5
77	1353.0
78	817.5
79	513.5
80	321.5
81	182.0
82	102.0
83	55.5
84	36.0
85	33.5
86	30.5
87	19.0
88	11.5
89	8.0
90	5.0
91	1.0
92	1.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35200867736399605
2	3.9783982523055613E-4
3	0.0023154277828418366
4	0.001360612202288502
5	0.004957084222372729
6	0.0
7	1.1139515106455572E-4
8	2.466606916429448E-4
9	0.003111107433302949
10-11	7.956796504611123E-5
12-13	8.752476155072234E-5
14-15	2.1881190387680586E-4
16-17	1.153735493168613E-4
18-19	0.001042340342104057
20-21	2.7053108115677815E-4
22-23	1.5117913358761133E-4
24-25	0.0
26-27	0.00212446466673117
28-29	0.01138219739984621
30-31	0.003520882453290422
32-33	0.014067616220152466
34-35	0.011139515106455573
36-37	0.012929794319993073
38-39	0.01283431276193774
40-41	0.01399202665335866
42-43	0.006317696424661232
44-45	0.0012850226354946964
46-47	0.00595964058195373
48-49	0.012973556700768437
50-51	0.0036481911973641995
52-53	0.005820396643123036
54-55	0.005124176948969563
56-57	2.4268229339063924E-4
58-59	6.683709063873343E-4
60-61	3.9783982523055613E-4
62-63	0.0011020163158886405
64-65	4.137534182397784E-4
66-67	0.0014282449725776965
68-69	0.0016749056642206413
70-71	8.871828102641401E-4
72-73	0.0027371379975862265
74-75	0.007972710097620345
76-77	0.0036362560026072833
78-79	0.0016271648851929746
80-81	5.012781797905008E-4
82-83	2.1085510737219477E-4
84-85	0.0015277049288853356
86-87	0.004670639548206729
88-89	0.0017584520275190582
90-91	3.0235826717522267E-4
92-93	0.002060810294694281
94-95	0.0032503513721336436
96-97	0.001901674364602058
98-99	0.002140378259740392
100	0.0026257428465216704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2567872E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.63061491200651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.09631926369215	34.983891324770546
2	15.693578194820168	15.891510283468048
3	6.376228296873747	9.684970784701614
4	3.0572996066414704	6.191718362179731
5	1.6896848175139045	4.277489065910525
6	1.016205741548581	3.0870672943029733
7	0.6565440138307309	2.326885899593277
8	0.46409252444393445	1.879783191092945
9	0.34512577916928444	1.5726537379193617
>10	1.5007067614436476	13.111782408300027
>50	0.06980829796558549	2.3728636501269134
>100	0.03243937668558312	2.8787627347581686
>500	0.00128236010580938	0.4400471525973311
>1k	5.965827436916983E-4	0.4400358855131803
>5k	2.209563040374315E-5	0.05783758381265404
>10k+	6.628689121122946E-5	0.8027006409527181
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30648	0.2438598992733217	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25451	0.2025084278388577	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15653	0.1245477356866779	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.956796504611122E-6	7.956796504611122E-6
2	0.0	0.0	0.0	1.5913593009222244E-5	7.956796504611122E-6
3	0.0	0.0	0.0	2.387038951383337E-5	7.956796504611122E-6
4	0.0	0.0	0.0	2.387038951383337E-5	7.956796504611122E-6
5	0.0	0.0	0.0	2.387038951383337E-5	7.956796504611122E-6
6	0.0	0.0	0.0	2.387038951383337E-5	3.978398252305561E-5
7	0.0	0.0	0.0	2.387038951383337E-5	3.978398252305561E-5
8	0.0	0.0	0.0	2.387038951383337E-5	4.774077902766674E-5
9	0.0	0.0	0.0	2.387038951383337E-5	7.956796504611123E-5
10-11	0.0	0.0	0.0	2.387038951383337E-5	7.956796504611123E-5
12-13	0.0	0.0	0.0	2.387038951383337E-5	8.354636329841678E-5
14-15	0.0	3.978398252305561E-6	0.0	2.387038951383337E-5	1.5913593009222245E-4
16-17	0.0	7.956796504611122E-6	0.0	3.182718601844449E-5	1.9096311611066695E-4
18-19	0.0	7.956796504611122E-6	0.0	4.376238077536117E-5	1.949415143629725E-4
20-21	0.0	7.956796504611122E-6	0.0	9.15031598030279E-5	2.1085510737219477E-4
22-23	0.0	7.956796504611122E-6	0.0	4.1375341823977836E-4	2.506390898952504E-4
24-25	0.0	1.9891991261527806E-5	0.0	0.001105994714140946	2.983798689229171E-4
26-27	0.0	2.387038951383337E-5	0.0	0.0023552117653648924	3.0235826717522267E-4
28-29	0.0	2.387038951383337E-5	0.0	0.007272512005214567	3.103150636798338E-4
30-31	0.0	2.387038951383337E-5	0.0	0.0276100838710006	3.103150636798338E-4
32-33	0.0	2.387038951383337E-5	0.0	0.07415734342297567	3.4214224969827825E-4
34-35	0.0	2.387038951383337E-5	0.0	0.1414201226747058	3.739694357167228E-4
36-37	0.0	4.376238077536117E-5	0.0	0.24921482332092498	3.739694357167228E-4
38-39	0.0	6.763277028919455E-5	0.0	0.40719701791997887	3.8590463047363945E-4
40-41	0.0	7.161116854150011E-5	0.0	0.58431132971437	4.018182234828617E-4
42-43	0.0	7.956796504611123E-5	0.0	0.770456605541495	4.177318164920839E-4
44-45	0.0	7.956796504611123E-5	0.0	0.9882739098552245	4.4955900251052844E-4
46-47	0.0	7.956796504611123E-5	0.0	1.2354159876866984	4.972997815381951E-4
48-49	7.956796504611122E-6	7.956796504611123E-5	0.0	1.496072684381254	5.331053658089452E-4
50-51	7.956796504611122E-6	8.354636329841678E-5	0.0	1.7719069704083554	5.331053658089452E-4
52-53	7.956796504611122E-6	8.752476155072236E-5	0.0	2.0470967559185835	5.967597378458342E-4
54-55	7.956796504611122E-6	8.752476155072236E-5	0.0	2.3475453919326994	6.643925081350287E-4
56-57	1.1935194756916682E-5	8.752476155072236E-5	0.0	2.6838433745983408	6.842844993965565E-4
58-59	1.5913593009222244E-5	8.752476155072236E-5	0.0	3.049859992208705	7.001980924057788E-4
60-61	1.5913593009222244E-5	8.752476155072236E-5	0.0	3.431802138023048	7.161116854150011E-4
62-63	1.5913593009222244E-5	8.752476155072236E-5	0.0	3.821657317961227	7.399820749288344E-4
64-65	1.5913593009222244E-5	8.752476155072236E-5	0.0	4.234698602913843	7.4396047318114E-4
66-67	1.5913593009222244E-5	9.548155805533347E-5	0.0	4.6844087845579585	7.519172696857511E-4
68-69	1.5913593009222244E-5	9.548155805533347E-5	0.0	5.169208438787409	7.598740661903622E-4
70-71	1.5913593009222244E-5	9.945995630763903E-5	0.0	5.684279725318653	8.036364469657234E-4
72-73	1.5913593009222244E-5	1.0343835455994459E-4	0.0	6.212977025864044	9.150315980302791E-4
74-75	1.5913593009222244E-5	1.0343835455994459E-4	0.0	6.751445272517097	9.190099962825847E-4
76-77	1.5913593009222244E-5	1.1139515106455572E-4	0.0	7.334563082755777	9.269667927871958E-4
78-79	1.5913593009222244E-5	1.1139515106455572E-4	0.0	7.953490455663458	9.309451910395014E-4
80-81	1.5913593009222244E-5	1.1139515106455572E-4	0.0	8.612098372739634	9.508371823010291E-4
82-83	1.5913593009222244E-5	1.1537354931686128E-4	0.0	9.29041129635948	0.0010502971386086681
84-85	1.5913593009222244E-5	1.1935194756916684E-4	0.0	9.97332324835899	0.0010542755368609736
86-87	1.5913593009222244E-5	1.1935194756916684E-4	0.0	10.68972535684641	0.0010741675281225015
88	1.5913593009222244E-5	1.1935194756916684E-4	0.0	11.245929302908241	0.0010741675281225015
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18425	0.0	53.68622	1
GTATCAA	34540	0.0	43.770107	1
CAACGCA	42385	0.0	34.906	5
ATCAACG	42690	0.0	34.603218	3
TATCAAC	45055	0.0	33.411686	2
AACGCAG	46765	0.0	32.197704	6
TCAACGC	48435	0.0	30.75028	4
ACGCAGA	50385	0.0	29.11957	7
CGCAGAG	51890	0.0	28.175373	8
GCAGAGT	57045	0.0	25.250381	9
GTACATG	45790	0.0	25.135706	1
GTGGTAT	6235	0.0	25.04168	1
TACATGG	46760	0.0	24.303354	2
ACATGGG	47665	0.0	23.290485	3
GAGTACT	30735	0.0	21.857473	12-13
CATGGGG	36665	0.0	21.573399	4
AGAGTAC	52145	0.0	21.453388	10-11
CAGAGTA	55580	0.0	21.0153	10-11
GTACTAG	5470	0.0	20.782684	1
TAGTACT	7160	0.0	20.479895	4
>>END_MODULE