FastQCFastQC Report
Thu 26 May 2016
ERR580127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6845075
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210360.3073158438731497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160410.23434367044919158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103890.15177335529559574No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97600.050.0161551
GTATCAA174000.041.6230851
CAACGCA204550.034.252515
ATCAACG208650.033.448873
TATCAAC222450.032.1349142
AACGCAG225950.031.3694766
TCAACGC226100.031.198784
GTACATG228550.029.4868051
GTACTAG35600.029.3268741
TACATGG233700.028.2938842
ACGCAGA249500.028.1446067
CGCAGAG258200.027.0874528
ACATGGG235350.027.0364423
TACCTGG46250.026.946362
CATGGGG187450.025.689844
GTGGTAT39400.024.827321
ATGGGGG137150.024.4798095
GCAGAGT286150.024.0313639
CTAACGC16000.023.5145113
CTGTGCG48250.023.2824349
Produced by FastQC (version 0.11.5)