FastQCFastQC Report
Thu 26 May 2016
ERR580127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6845075
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182840.26711175553226224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145860.21308751182419478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101460.14822335766956535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98950.049.2662931
GTATCAA174600.041.0055431
CAACGCA210050.032.7350775
ATCAACG214750.032.0414623
TATCAAC226550.031.0995922
AACGCAG231400.030.079296
TCAACGC231500.029.823994
GTACATG224700.028.8083111
TACATGG229400.027.6808262
ACATGGG231250.026.4016973
TACCTGG44750.026.3631822
ACGCAGA261000.026.217837
CGCAGAG270900.025.2600738
CATGGGG180550.024.7037934
GTACTAG34700.024.520881
GTGGTAT38400.023.6272411
ATGGGGG132600.023.6060035
TGGGGGG116300.023.4381946
GTACCTG54100.022.5924341
GCAGAGT299900.022.5040259
Produced by FastQC (version 0.11.5)