FastQCFastQC Report
Thu 26 May 2016
ERR580126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5204594
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149470.2871885876208595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112090.21536742347241689No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73070.1403951970124855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79600.044.7865221
GTATCAA144200.042.074181
GTACTAG43900.037.603981
CAACGCA160400.036.180355
ATCAACG165400.035.031453
GCATCGT43050.034.0647668
TATCAAC180400.033.5270732
AACGCAG185250.032.1493076
TAGGCAT56150.030.570685
TCAACGC192700.030.2399334
ACGCAGA193150.030.0066417
GGGCACC49200.029.99399294
CTGTGCG57300.029.448269
CGCAGAG199450.028.986458
TAGTACT57300.028.9741924
GTGGTAT25650.028.787471
CTAGGCA63050.028.4204624
GTACATG197100.027.560471
TACATGG200150.026.5765292
CTAGTAC65950.026.4570833
Produced by FastQC (version 0.11.5)