FastQCFastQC Report
Thu 26 May 2016
ERR580126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5204594
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131200.25208498491909265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104650.20107236030322442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69400.13334373440080052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82550.043.735641
GTATCAA144100.039.3762741
GTACTAG40800.034.9119261
CAACGCA164200.033.2620665
ATCAACG167800.032.548463
TATCAAC183550.030.8325332
GGGCACC48850.030.6955794
AACGCAG190550.029.6218856
TAGGCAT51850.028.7360275
TCAACGC195650.028.0834774
CTGTGCG59850.027.562699
ACGCAGA200350.027.2111767
GTGGTAT28700.026.5353281
CGCAGAG205500.026.4148928
TAGTACT62850.026.0997524
CTAGGCA59050.026.0281964
GCATCGT51100.025.38448
GTACATG193250.025.0558471
CGACGAG26250.024.61796224-25
TACGGGC34400.024.5940634
Produced by FastQC (version 0.11.5)