FastQCFastQC Report
Thu 26 May 2016
ERR580125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9460336
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141260.14931816375232337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112470.11888584084117097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144200.042.4221881
GTATCAA241050.038.0567051
CAACGCA253850.033.4603925
GTACTAG80700.032.278791
ATCAACG264550.032.1767583
TATCAAC298500.030.3795662
AACGCAG300300.029.2416466
GTACATG410600.028.0332531
GTGGTAT44950.027.8248581
ACGCAGA311300.027.2129697
TAGGCAT101050.027.212575
TACATGG420250.027.0036982
TCAACGC322150.026.6580354
ACATGGG411300.026.5931223
GGGCACC91200.026.59304694
GCATCGT87750.026.4505428
CTGTGCG105850.026.3701349
CGCAGAG319500.026.3672088
TAGTACT107550.026.2233734
CTAGGCA111050.025.3968834
Produced by FastQC (version 0.11.5)