FastQCFastQC Report
Thu 26 May 2016
ERR580125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9460336
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128170.13548144590213287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107750.113896588873799No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144950.043.0738751
GTATCAA244750.037.9191471
CAACGCA261000.033.9093865
ATCAACG268850.032.937293
GTACTAG77750.031.6858221
TATCAAC302800.030.50992
AACGCAG306000.029.9040076
GGGCACC91300.028.31255594
GTGGTAT43550.027.5287271
TCAACGC330000.027.2466724
ACGCAGA323200.027.2219587
GTACATG395750.026.622881
CGCAGAG331600.026.4330278
TAGGCAT102850.026.0483785
TACATGG406000.025.6380922
TAGTACT112500.025.4852984
CTGTGCG111400.025.4811049
ACATGGG396450.025.0511743
CTAGGCA112250.024.7046134
TACCCCG7700.023.8058665
Produced by FastQC (version 0.11.5)