FastQCFastQC Report
Thu 26 May 2016
ERR580124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4318177
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128980.2986908595919065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127780.2959119091227618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80770.1870465244940168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64050.056.670951
GTATCAA108950.036.854711
GTACATG147200.031.4942651
CAACGCA130050.030.1128395
TACATGG152550.029.7421552
ATCAACG131600.029.6891273
ACATGGG149950.029.5363433
TATCAAC139000.028.480962
AACGCAG140050.028.3865836
TCAACGC141350.027.9066934
CATGGGG114050.027.8672184
ATGGGGG78900.026.336765
ACGCAGA158100.024.789927
CGCAGAG162850.023.9225648
CTAACGC8900.023.7724973
GAGTACA104100.023.5314881
TCTAACG9050.023.3787482
AGTACAT102450.023.2676932
TAACGCC9350.023.1306844
GTACTAG19950.022.8607861
Produced by FastQC (version 0.11.5)