FastQCFastQC Report
Thu 26 May 2016
ERR580124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4318177
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120580.27923820630789337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113230.26221713468438185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77360.1791496735775305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63650.056.0346761
GTATCAA105300.036.1558531
GTACATG140550.029.5047191
CAACGCA129650.028.564355
TACATGG142900.028.1867122
ATCAACG131250.028.0751863
CTAACGC7650.027.647573
ACATGGG141600.027.516753
TATCAAC140800.026.8045642
TCAACGC139200.026.7400154
AACGCAG140000.026.7202556
TCTAACG8500.025.9881132
CATGGGG108700.024.601324
TAACGCC8400.024.0582984
GAGTACA100000.023.9660911
ACGCAGA155800.023.618377
ATGGGGG74850.023.1061025
CGCAGAG161400.022.7697778
AGTACAT101150.022.6751292
CGCCTAC9250.021.8465237
Produced by FastQC (version 0.11.5)