FastQCFastQC Report
Thu 26 May 2016
ERR580123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8769880
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171220.19523642284729095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150210.1712794245759349No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123900.048.763031
GTATCAA210450.038.7176061
CAACGCA243900.032.2278485
ATCAACG250450.031.5189113
TATCAAC265050.030.2806072
AACGCAG267800.029.689876
TCAACGC270800.029.3748654
GTGGTAT51700.028.9198111
GTACTAG48600.026.9914861
GTACATG315550.026.462671
ACGCAGA296600.026.2358637
TACATGG317300.025.7980922
CGCAGAG305750.025.3886628
CTGTGCG63400.024.8336739
TAGTACT62300.024.6010534
ACATGGG322900.024.4760423
TGGTATC60450.023.2559322
CTAGTAC68700.022.7891983
CATGGGG251300.022.6181964
GCAGAGT341500.022.2538269
Produced by FastQC (version 0.11.5)