FastQCFastQC Report
Thu 26 May 2016
ERR580123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8769880
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152910.1743581440110925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139260.15879350686668461No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125050.050.1958771
GTATCAA209900.037.7908971
CAACGCA241400.031.792115
ATCAACG249200.030.7977143
TATCAAC269250.029.4638612
AACGCAG267750.029.206336
TCAACGC271800.028.391884
GTACTAG47500.027.6011
GTACATG323700.025.9474641
ACGCAGA297000.025.760317
TAGTACT68150.025.377714
TACATGG325100.025.1538942
CTGTGCG67450.024.9433179
CGCAGAG308850.024.711078
ACATGGG323650.024.3521773
CTAGTAC71800.023.8263383
CATGGGG252850.022.0626754
GTGGTAT54750.022.0510621
GCAGAGT350200.021.6188479
CTAGGCA69950.020.9622234
Produced by FastQC (version 0.11.5)