FastQCFastQC Report
Thu 26 May 2016
ERR580122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5979500
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79650.13320511748473954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64830.10842043649134543No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82750.045.4004021
GTATCAA144400.040.4096871
CAACGCA163850.034.229925
ATCAACG167800.033.4829833
TATCAAC177150.032.4350552
AACGCAG183200.031.6012676
TCAACGC180800.031.1004314
ACGCAGA204400.027.2194397
CGCAGAG209450.026.3385878
GTACATG245750.024.2800181
GTACTAG29850.023.7855681
TACATGG253000.023.2127442
GCAGAGT233850.022.9110419
ACATGGG253850.022.2996223
GCATCGT28450.022.29758
GTGGTAT38350.020.9658091
CATGGGG195850.019.9725444
GAGTACT90700.019.23004312-13
CTGTGCG43200.019.1472459
CAGAGTA226800.018.97340810-11
Produced by FastQC (version 0.11.5)