FastQCFastQC Report
Thu 26 May 2016
ERR580122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5979500
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69430.11611338740697381No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60290.10082782841374696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83450.044.0827061
GTATCAA143450.038.532491
CAACGCA161650.032.300585
ATCAACG164900.031.7500063
TATCAAC177200.030.7126162
TCAACGC177150.029.686634
AACGCAG181400.029.507546
ACGCAGA204500.025.4390497
CGCAGAG210300.024.513788
GTACATG245150.024.4904371
GTACTAG32200.024.4602361
TACATGG252100.023.5638472
ACATGGG252100.022.5948263
GCAGAGT237850.021.1211459
GTGGTAT40650.020.999941
CATGGGG190350.020.4679154
CGTTAAC10200.019.8823781
TGGTATC43050.018.9953842
TAGTACT43650.018.626614
TAGGCAT46500.018.596745
Produced by FastQC (version 0.11.5)