##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580122_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5979500
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.510977673718536	34.0	31.0	34.0	31.0	34.0
2	32.71634785517184	34.0	31.0	34.0	31.0	34.0
3	32.79864938540012	34.0	31.0	34.0	31.0	34.0
4	36.270803411656495	37.0	37.0	37.0	35.0	37.0
5	36.19991587925412	37.0	37.0	37.0	35.0	37.0
6	36.225034367422026	37.0	37.0	37.0	35.0	37.0
7	36.19361568693034	37.0	37.0	37.0	35.0	37.0
8	36.1826092482649	37.0	37.0	37.0	35.0	37.0
9	37.964577138556734	39.0	38.0	39.0	35.0	39.0
10-11	37.91535546450372	39.0	38.0	39.0	35.0	39.0
12-13	37.879445522200854	39.0	38.0	39.0	35.0	39.0
14-15	39.27815210301865	41.0	39.0	41.0	36.0	41.0
16-17	39.26263199264153	41.0	39.0	41.0	36.0	41.0
18-19	39.253042143991976	41.0	39.0	41.0	36.0	41.0
20-21	39.23332812108036	41.0	39.0	41.0	36.0	41.0
22-23	39.163773308805084	40.5	39.0	41.0	36.0	41.0
24-25	39.08452362237645	40.0	39.0	41.0	35.0	41.0
26-27	38.950971736767286	40.0	38.0	41.0	35.0	41.0
28-29	38.84598570114558	40.0	38.0	41.0	35.0	41.0
30-31	38.748674220252525	40.0	38.0	41.0	35.0	41.0
32-33	38.62270382138975	40.0	38.0	41.0	34.5	41.0
34-35	38.60627611004264	40.0	38.0	41.0	34.5	41.0
36-37	38.670086629316835	40.0	38.0	41.0	35.0	41.0
38-39	38.58851174847395	40.0	38.0	41.0	34.5	41.0
40-41	38.47402441675725	40.0	38.0	41.0	34.0	41.0
42-43	38.459660673969395	40.0	38.0	41.0	34.0	41.0
44-45	38.3887166151016	40.0	38.0	41.0	34.0	41.0
46-47	38.22209515845806	40.0	38.0	41.0	34.0	41.0
48-49	38.03343532067899	40.0	37.0	41.0	33.0	41.0
50-51	37.87056066560749	40.0	37.0	41.0	33.0	41.0
52-53	37.696174763776234	40.0	36.5	41.0	33.0	41.0
54-55	37.49487356802408	39.5	36.0	41.0	33.0	41.0
56-57	37.282109039217325	39.0	35.5	41.0	32.0	41.0
58-59	37.045844970315244	39.0	35.0	41.0	32.0	41.0
60-61	36.77856049836943	38.5	35.0	40.5	31.5	41.0
62-63	36.4852649050924	38.0	35.0	40.0	31.0	41.0
64-65	36.18564236140145	37.0	35.0	40.0	31.0	41.0
66-67	35.84784388326783	37.0	35.0	39.5	31.0	41.0
68-69	35.48287356802408	36.0	35.0	39.0	30.5	41.0
70-71	35.141518270758425	36.0	34.0	39.0	30.0	40.5
72-73	34.78126875156786	35.0	34.0	38.0	30.0	40.0
74-75	34.421582908269926	35.0	34.0	37.0	29.5	39.0
76-77	33.1908912952588	34.5	32.5	36.0	28.0	39.0
78-79	33.82198269086044	35.0	34.0	36.0	29.0	38.5
80-81	33.76068241491764	35.0	34.0	36.0	29.0	37.0
82-83	33.56100409733255	35.0	34.0	35.5	29.0	37.0
84-85	33.35668818463082	35.0	34.0	35.0	29.0	36.5
86-87	33.194760013379046	35.0	34.0	35.0	29.0	36.0
88-89	33.04665950330295	35.0	33.0	35.0	29.0	36.0
90-91	32.89976804080609	35.0	33.0	35.0	29.0	36.0
92-93	32.7916554059704	35.0	33.0	35.0	29.0	35.0
94-95	32.70036365916883	35.0	33.0	35.0	29.0	35.0
96-97	32.610178359394595	35.0	33.0	35.0	28.0	35.0
98-99	32.54272263567188	35.0	33.0	35.0	27.0	35.0
100	31.25491462496864	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1162202273824775
1101	2	0.07648446788502383
1101	3	0.04562482868637119
1101	4	0.01791806805661622
1101	5	0.02404144582876455
1101	6	0.06108473278224125
1101	7	0.03037149518857518
1101	8	0.04789580330080412
1101	9	0.014988323451440522
1101	10-11	0.02431355151545489
1101	12-13	-0.006680750513808675
1101	14-15	0.03795442662832471
1101	16-17	0.03572020028075684
1101	18-19	-0.028287880651568287
1101	20-21	-0.016532258172809122
1101	22-23	-0.049699974694085824
1101	24-25	-0.07988650100151773
1101	26-27	-0.09627292896523443
1101	28-29	-0.1278040662227582
1101	30-31	-0.13599538334484151
1101	32-33	-0.1296226650218344
1101	34-35	-0.11496484586114519
1101	36-37	-0.046622214066317724
1101	38-39	-0.04281150843338821
1101	40-41	-0.0193650905986118
1101	42-43	-0.02523123053008902
1101	44-45	-0.09968350997672104
1101	46-47	-0.009261169190445173
1101	48-49	-0.09066147784187706
1101	50-51	-0.11872723199667945
1101	52-53	-0.1492720056890917
1101	54-55	-0.11670174106518516
1101	56-57	-0.1086777765587783
1101	58-59	-0.09717360087141458
1101	60-61	-0.20653112554297337
1101	62-63	-0.04040015420189036
1101	64-65	-0.08821153208169363
1101	66-67	-0.11482371762240007
1101	68-69	-0.10916160277825071
1101	70-71	-0.1427706130398505
1101	72-73	-0.15074921650669637
1101	74-75	-0.13413696395404884
1101	76-77	-0.10811987639791454
1101	78-79	-0.10431959479285524
1101	80-81	-0.11240433798607086
1101	82-83	-0.10421556885944483
1101	84-85	-0.04957725306113048
1101	86-87	-0.08401141282844549
1101	88-89	-0.09142159164287733
1101	90-91	-0.08882855511485133
1101	92-93	-0.17699909030243788
1101	94-95	-0.1315023950852634
1101	96-97	-0.11272477845054141
1101	98-99	-0.17446777972380545
1101	100	-0.08220298583143304
1102	1	0.07236834199257913
1102	2	0.021508502025426424
1102	3	-0.011781797359297741
1102	4	0.01806067612286455
1102	5	-0.00585058887435963
1102	6	0.05672871444504324
1102	7	-0.012503056823994996
1102	8	0.016044922653257743
1102	9	-0.010150452786270137
1102	10-11	-0.010567214572368755
1102	12-13	-0.03454110196521043
1102	14-15	0.030533067581863804
1102	16-17	-0.05435044376604026
1102	18-19	-0.0441545952179041
1102	20-21	-0.029765811812758614
1102	22-23	-0.07157515651597635
1102	24-25	-0.09838792333719226
1102	26-27	-0.07861807750123972
1102	28-29	-0.11100817394294893
1102	30-31	-0.10255387322229836
1102	32-33	-0.1267219681066578
1102	34-35	-0.13807607733050986
1102	36-37	-0.040206153689730684
1102	38-39	-0.08685031758357553
1102	40-41	-0.020987579611144724
1102	42-43	-0.018827789135563933
1102	44-45	-0.039450164102198926
1102	46-47	-0.005844844385123338
1102	48-49	-0.07022964976223989
1102	50-51	-0.024373189819215213
1102	52-53	-0.0461709600449538
1102	54-55	-0.09555390108755546
1102	56-57	-0.20221125925019834
1102	58-59	-0.09390316778144125
1102	60-61	-0.06134266742301975
1102	62-63	-0.02898810313371314
1102	64-65	-0.135336984953895
1102	66-67	-0.14670275352565199
1102	68-69	-0.10894581818476468
1102	70-71	-0.12191748735326513
1102	72-73	-0.1532831081074093
1102	74-75	-0.15790071891589008
1102	76-77	-0.14913094397790871
1102	78-79	-0.10114868883569272
1102	80-81	-0.07808795247937184
1102	82-83	-0.13949707582534643
1102	84-85	-0.15771929709958243
1102	86-87	-0.1955288310400718
1102	88-89	-0.11488501483132296
1102	90-91	-0.12449620293224228
1102	92-93	-0.12568511233468627
1102	94-95	-0.20628991856472112
1102	96-97	-0.2528548836836251
1102	98-99	-0.25979256030006326
1102	100	-0.028673508106408008
1103	1	0.07206680615200867
1103	2	0.06475790522504354
1103	3	0.04575345760027005
1103	4	-8.07358061322816E-4
1103	5	-0.04985533192095204
1103	6	-0.005385911667815435
1103	7	-0.01075498692368626
1103	8	-0.00140106796618511
1103	9	-0.060231433608429086
1103	10-11	-0.04241861706137939
1103	12-13	-0.02517366333795934
1103	14-15	-0.005085383913872477
1103	16-17	0.009306583449010475
1103	18-19	-0.015217967103858143
1103	20-21	-0.10097202059115062
1103	22-23	-0.13180233856501644
1103	24-25	-0.10842814775879361
1103	26-27	-0.08657819118904087
1103	28-29	-0.11658114015601484
1103	30-31	-0.1373214557217466
1103	32-33	-0.22833639721429932
1103	34-35	-0.13942919069415183
1103	36-37	-0.0780432926549608
1103	38-39	-0.04884298860022085
1103	40-41	-0.1578765660895769
1103	42-43	-0.16983615418040188
1103	44-45	-0.17264522164999363
1103	46-47	-0.13922266157595686
1103	48-49	-0.17556413369830892
1103	50-51	-0.12691543294960184
1103	52-53	-0.15389966018030776
1103	54-55	-0.18593566735719236
1103	56-57	-0.12243733733476603
1103	58-59	-0.14654982529922478
1103	60-61	-0.12437660616715362
1103	62-63	-0.14288593462742938
1103	64-65	-0.14559975931501867
1103	66-67	-0.1780467486401065
1103	68-69	-0.17119177660433849
1103	70-71	-0.1525703017706732
1103	72-73	-0.19651380554265074
1103	74-75	-0.2161474957396834
1103	76-77	-0.29222368910397023
1103	78-79	-0.22368882675664992
1103	80-81	-0.16281087361478797
1103	82-83	-0.09258542455614815
1103	84-85	-0.1657095373399926
1103	86-87	-0.1543112739177488
1103	88-89	-0.12682088736224983
1103	90-91	-0.10157582980306756
1103	92-93	-0.11473064367895347
1103	94-95	-0.12962437027930207
1103	96-97	-0.18428789066445006
1103	98-99	-0.12688830710661847
1103	100	-0.009023372938337104
1104	1	0.10096870516014178
1104	2	0.017276580947346076
1104	3	0.024268834957858587
1104	4	-0.00965651996013861
1104	5	-0.013602941437241611
1104	6	0.003963207820952164
1104	7	-0.005006676985836123
1104	8	-0.032951299385118205
1104	9	-0.01633036968468815
1104	10-11	-0.029248439979767227
1104	12-13	-0.08123968790780367
1104	14-15	-0.025623869062044946
1104	16-17	-0.024537561385230333
1104	18-19	-0.08107092929245852
1104	20-21	-0.12181595643567533
1104	22-23	-0.11313995137792432
1104	24-25	-0.14497516781917597
1104	26-27	-0.11278912913308403
1104	28-29	-0.1671781817711917
1104	30-31	-0.2060389541062264
1104	32-33	-0.2099888608881244
1104	34-35	-0.20180004773556703
1104	36-37	-0.1392169403647756
1104	38-39	-0.13868287537851387
1104	40-41	-0.1522865833421605
1104	42-43	-0.16322740127181845
1104	44-45	-0.2077864798904372
1104	46-47	-0.11556796722121732
1104	48-49	-0.14976275873842582
1104	50-51	-0.15043180278966872
1104	52-53	-0.17131745464811843
1104	54-55	-0.28900453689777095
1104	56-57	-0.1721038719619159
1104	58-59	-0.23624461070002667
1104	60-61	-0.262511587283754
1104	62-63	-0.23601686274284361
1104	64-65	-0.27346292920790916
1104	66-67	-0.18968535472248504
1104	68-69	-0.20605310668827315
1104	70-71	-0.20544267508050496
1104	72-73	-0.20770131302999317
1104	74-75	-0.27658847713434653
1104	76-77	-0.36595978232562487
1104	78-79	-0.2252916641198084
1104	80-81	-0.2086793045393236
1104	82-83	-0.11727768940119176
1104	84-85	-0.0822386446216612
1104	86-87	-0.08375101536513085
1104	88-89	-0.1903408598700409
1104	90-91	-0.10819365496350741
1104	92-93	-0.18464021409580056
1104	94-95	-0.17600194034987737
1104	96-97	-0.1590690526472045
1104	98-99	-0.12571869709540096
1104	100	-0.2229674021724115
1105	1	0.0336584379812237
1105	2	0.028844105354771443
1105	3	-0.0020980732064757035
1105	4	-0.026270085633413487
1105	5	-0.05902198740905362
1105	6	-0.030585438187188174
1105	7	-0.004447897825642144
1105	8	-0.015885022979034602
1105	9	-0.06891777151574274
1105	10-11	-0.04131664520625833
1105	12-13	-0.08415311937613268
1105	14-15	-0.011878686382829073
1105	16-17	-0.042632217384053206
1105	18-19	-0.056581532462310236
1105	20-21	-0.07862067455521782
1105	22-23	-0.05310819874240025
1105	24-25	-0.06528786711485424
1105	26-27	-0.11826011190547803
1105	28-29	-0.08376084513716364
1105	30-31	-0.10223360736708997
1105	32-33	-0.07719124545774747
1105	34-35	-0.09528113561253804
1105	36-37	-0.08586343622231851
1105	38-39	-0.09597842431100645
1105	40-41	-0.10838246274514063
1105	42-43	-0.03719715741237195
1105	44-45	-0.031149909722664404
1105	46-47	-0.12074225334063016
1105	48-49	-0.12244187469982393
1105	50-51	-0.08519281938153256
1105	52-53	-0.1021083622799921
1105	54-55	-0.1184271395565375
1105	56-57	-0.11804091695562846
1105	58-59	-0.12421865476527927
1105	60-61	-0.04647201541288126
1105	62-63	-0.07299269494592409
1105	64-65	-0.06885417812858208
1105	66-67	-0.05790688102600683
1105	68-69	-0.06902252069416193
1105	70-71	-0.041679116853167386
1105	72-73	-0.03308001515757297
1105	74-75	-0.10425513534225672
1105	76-77	-0.13998979045270943
1105	78-79	-0.006371355009925139
1105	80-81	0.0011544574084041415
1105	82-83	0.02491112775886961
1105	84-85	-0.012256048525109975
1105	86-87	-0.040623347933397724
1105	88-89	-0.050950668739851324
1105	90-91	-0.06644854997134075
1105	92-93	-0.08113540384253781
1105	94-95	-0.038108671899699686
1105	96-97	-0.04807044678479144
1105	98-99	-0.12142331533475925
1105	100	-0.005829693744612285
1106	1	0.09799738889042686
1106	2	0.08594050246092166
1106	3	0.0025610689821604637
1106	4	0.0013318911265329803
1106	5	-0.01078370040968224
1106	6	0.02992128184650511
1106	7	0.025513654304234024
1106	8	0.04648266876486673
1106	9	0.04100979533644278
1106	10-11	0.006235783188692778
1106	12-13	-0.010571492689983586
1106	14-15	0.03859282645992579
1106	16-17	-0.029335730243332137
1106	18-19	-0.008090410520914304
1106	20-21	-0.01689573744733508
1106	22-23	-0.021089587177215208
1106	24-25	-0.016500833440815654
1106	26-27	-0.04220671994929859
1106	28-29	0.02143996119512792
1106	30-31	-0.04040756889573771
1106	32-33	-0.047010993684914126
1106	34-35	-2.574588690364976E-4
1106	36-37	0.017036471579764623
1106	38-39	-0.019268716003658426
1106	40-41	-0.030137019291181844
1106	42-43	-0.010643291964804291
1106	44-45	-1.62831020475096E-4
1106	46-47	-0.06203943166679693
1106	48-49	-0.02832677851601062
1106	50-51	-0.048806547381254006
1106	52-53	-0.07520980097413599
1106	54-55	-0.13427382705733493
1106	56-57	-0.1267776510706753
1106	58-59	-0.1602069896247329
1106	60-61	-0.2106500303452279
1106	62-63	-0.20538734922763524
1106	64-65	-0.17541534135520465
1106	66-67	-0.11181698169361454
1106	68-69	-0.0436269996584997
1106	70-71	-0.12403367846515323
1106	72-73	-0.1533363636437528
1106	74-75	-0.177507557508072
1106	76-77	-0.13851371054611405
1106	78-79	-0.012567645590024767
1106	80-81	-0.00164056044685168
1106	82-83	-0.01779903452621312
1106	84-85	0.010080775776692974
1106	86-87	0.060942728493365905
1106	88-89	0.05731367197217452
1106	90-91	0.04191240776948035
1106	92-93	0.011357477480011369
1106	94-95	0.011941612439727578
1106	96-97	0.03596549572831975
1106	98-99	0.03223678775166405
1106	100	0.12382264040865465
1107	1	0.03495742824936343
1107	2	-8.486989849743054E-4
1107	3	-0.037492139617818054
1107	4	-0.028708955400006175
1107	5	-0.04574556103694505
1107	6	-0.03161903666369881
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2316	8	-0.010567058549604269
2316	9	-0.01953783487174121
2316	10-11	-0.031275904295306134
2316	12-13	-0.05412826764175094
2316	14-15	-0.03625990578166949
2316	16-17	1.5963455726364373E-4
2316	18-19	-0.009364498013404443
2316	20-21	-0.010706049981912713
2316	22-23	-0.027996967044053633
2316	24-25	-0.029855039388493765
2316	26-27	0.0017984064177412051
2316	28-29	0.030884139018688472
2316	30-31	-0.2853247037516766
2316	32-33	-0.11022310867598861
2316	34-35	-0.17715572238407162
2316	36-37	-0.47627223590500734
2316	38-39	-0.29721844294736144
2316	40-41	-0.23221114804572807
2316	42-43	-0.38956218934588804
2316	44-45	-0.23691125958114156
2316	46-47	-0.39961905880196724
2316	48-49	-0.3734051116411834
2316	50-51	-0.354366633149823
2316	52-53	-0.34094068844277814
2316	54-55	-0.22491056072876603
2316	56-57	-1.058695609288236
2316	58-59	-0.7924056582381667
2316	60-61	-0.44651507583277095
2316	62-63	-0.18931779200684673
2316	64-65	-0.1686840387773998
2316	66-67	-0.09712140379721035
2316	68-69	-0.20894437532141552
2316	70-71	-0.255472905046652
2316	72-73	-0.12903707557653377
2316	74-75	-0.20187034615381094
2316	76-77	-0.14135342188043154
2316	78-79	-0.17526094015749294
2316	80-81	-0.2038542228661484
2316	82-83	-0.3433018318784349
2316	84-85	-0.18146174203177878
2316	86-87	-0.2561524576675751
2316	88-89	-0.35322766277773354
2316	90-91	-0.24068816198599308
2316	92-93	-0.24886949321810192
2316	94-95	-0.11324090886921567
2316	96-97	-0.2698098139566767
2316	98-99	-0.12199287391836577
2316	100	-0.4347760896204065
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	5.0
13	48.0
14	172.0
15	538.0
16	1206.0
17	2577.0
18	4662.0
19	7270.0
20	11185.0
21	16937.0
22	22654.0
23	25701.0
24	26596.0
25	30396.0
26	36174.0
27	45929.0
28	57072.0
29	71222.0
30	87897.0
31	110494.0
32	139582.0
33	184239.0
34	271153.0
35	413160.0
36	745400.0
37	1560078.0
38	1806475.0
39	300644.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.074513584327825	19.44956133137221	13.829054504467914	23.64687057983205
2	16.00606676451267	20.384034421814395	38.95350820742461	24.65639060624832
3	19.238842471133776	25.161065680078227	29.63369358729222	25.966398261495772
4	13.584882562306147	17.053978090873606	38.00214268058506	31.35899666623519
5	14.317906812026013	37.58001272744453	33.77447336226137	14.32760709826808
6	31.203261142235974	36.290659754160046	18.170783510326952	14.335295593277031
7	28.32662541236739	32.08102084823843	21.36548920996378	18.226864529430397
8	27.198313699801034	33.97065731959012	19.702746776852372	19.12828220375648
9	26.49694722760234	16.25967773253108	20.20698343741152	37.03639160245506
10-11	25.688504761478704	25.694533696184337	27.450458838723492	21.16650270361347
12-13	26.489391458061483	22.950456957294488	27.492318543489645	23.06783304115438
14-15	23.84435358941207	24.638593699227478	25.0181160965427	26.498936614817755
16-17	23.488983387804232	27.45458749216593	25.66785559142352	23.388573528606315
18-19	23.062353289034903	27.041795190322045	27.327657889261946	22.56819363138111
20-21	24.26728449006449	26.111757255409806	27.074473702716777	22.54648455180892
22-23	24.173120885882092	25.76428594662769	26.838583881743023	23.224009285747197
24-25	23.79250773476043	25.91994313905845	27.07544945229534	23.212099673885778
26-27	23.638545681507342	26.283553239149626	26.65448646999738	23.423414609345656
28-29	23.36686760131762	26.3515253329279	26.981893301668165	23.29971376408631
30-31	23.987625272127108	25.976877160689664	26.983101485579457	23.052396081603767
32-33	23.84895310943546	26.17953584209502	26.584737130984866	23.386773917484657
34-35	23.926174272137768	26.31726882105302	26.80515855161813	22.95139835519108
36-37	23.87290293811955	26.077830680202226	26.600985963220904	23.448280418457323
38-39	23.783029395060822	26.2070729824008	26.801691368629193	23.208206253909182
40-41	23.947298280868264	25.669499283991303	27.007272654589592	23.37592978055084
42-43	23.67356069234616	25.75838463712705	26.831223480496195	23.736831190030593
44-45	23.755920836704274	26.030366695724705	26.776777818891066	23.436934648679948
46-47	23.75068258315389	25.91420985512094	26.36518721384818	23.96992034787699
48-49	23.990077443243944	25.890406628463275	26.605796142004763	23.513719786288025
50-51	23.89569946782712	25.936579108451546	26.521944187073316	23.645777236648012
52-53	23.706946329583108	26.04830736406451	26.403750833835005	23.840995472517378
54-55	23.623592233504954	25.93483087568706	26.535053664819348	23.906523225988643
56-57	23.98778122809177	25.91017826275427	26.231426503406315	23.870614005747647
58-59	23.49428071216136	26.072005337606186	26.691066307916294	23.742647642316168
60-61	23.76419897241935	26.18606427780868	26.423936053323683	23.625800696448287
62-63	23.786106289269416	25.97336559495933	26.322645415424322	23.91788270034693
64-65	23.39906808043367	25.900386755650906	26.537515097451518	24.163030066463907
66-67	23.65974414232288	25.938736846964034	26.40413331197117	23.99738569874192
68-69	23.382292722285463	25.950421709561216	26.531733097814058	24.135552470339263
70-71	23.423730707049863	25.663718960152025	26.51260960245347	24.399940730344643
72-73	23.38354690812978	25.922311310672807	26.454453227671483	24.23968855352593
74-75	23.506017675329776	26.009776030703403	26.356647750217572	24.12755854374925
76-77	23.33282936924323	25.923182493090284	26.170713041174647	24.57327509649184
78-79	23.284429277543968	25.8570223241649	26.34669561050909	24.51185278778204
80-81	23.23252788875004	26.26643288905776	26.258162928098	24.242876294094195
82-83	23.53974955715272	25.851477584702902	26.664490337634263	23.944282520510118
84-85	23.48156619813378	25.91011704416632	26.29609161239082	24.31222514530908
86-87	22.928715097963316	26.170624811274518	26.560690226631134	24.339969864131035
88-89	23.14652591722943	25.681061039365023	26.593574293158866	24.57883875024668
90-91	23.223687648816057	25.701441804168002	26.48647563858943	24.588394908426515
92-93	23.25325262463137	25.757348587353825	26.52556580646084	24.463832981553974
94-95	22.845666111863334	25.71905622334134	26.704849534125756	24.730428130669562
96-97	22.83377278789012	25.658755369702085	26.71181570269302	24.795656139714772
98-99	22.616868601077154	25.72315453344584	26.651211317971253	25.008765547505757
100	22.85342673042394	25.518468246683916	26.40635828947122	25.221746733420925
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	88.0
1	66.0
2	85.5
3	151.0
4	124.5
5	112.5
6	253.5
7	474.5
8	744.5
9	927.0
10	896.0
11	859.5
12	927.5
13	1080.5
14	1274.0
15	1473.0
16	1723.0
17	2029.5
18	2332.5
19	2585.0
20	2903.5
21	3372.0
22	3971.5
23	4698.5
24	5483.0
25	6565.0
26	8317.0
27	10416.5
28	13054.5
29	16720.0
30	21816.5
31	28072.5
32	36949.0
33	48866.0
34	61744.0
35	76623.5
36	97625.0
37	122837.0
38	144822.0
39	163067.5
40	175112.0
41	183224.0
42	195826.0
43	213769.0
44	231643.5
45	253556.5
46	273784.0
47	289383.5
48	301733.5
49	302630.5
50	295612.5
51	287876.0
52	276855.0
53	265020.5
54	253745.0
55	230620.0
56	204242.5
57	175338.5
58	146271.0
59	126131.5
60	102986.0
61	79394.0
62	62061.5
63	47464.0
64	33760.0
65	22353.0
66	15300.5
67	10832.0
68	7873.5
69	5774.5
70	4204.0
71	3008.0
72	2057.0
73	1371.5
74	995.0
75	695.5
76	411.0
77	205.5
78	119.5
79	60.5
80	35.5
81	23.5
82	18.0
83	11.5
84	5.5
85	5.5
86	5.0
87	2.0
88	0.5
89	1.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3514675140061878
2	6.020570281796137E-4
3	0.0017894472782005184
4	0.0012375616690358726
5	0.004933522869805167
6	0.0
7	3.34476126766452E-5
8	3.177523204281294E-4
9	0.003027008947236391
10-11	4.1809515845806506E-5
12-13	4.1809515845806506E-5
14-15	1.8396186972154864E-4
16-17	1.254285475374195E-4
18-19	9.365331549460657E-4
20-21	2.2577138556735513E-4
22-23	2.2577138556735513E-4
24-25	0.0
26-27	0.0020403043732753574
28-29	0.011547788276611756
30-31	0.003629065975416005
32-33	0.014491178192156534
34-35	0.011539426373442595
36-37	0.013011121331214985
38-39	0.01323689271678234
40-41	0.014574797223848149
42-43	0.006856760598712267
44-45	0.0014633330546032278
46-47	0.006271427376870975
48-49	0.01336232126431976
50-51	0.004013713521197424
52-53	0.005844970315243749
54-55	0.00507567522368091
56-57	2.006856760598712E-4
58-59	6.773141567020654E-4
60-61	3.0102851408980686E-4
62-63	8.947236391002592E-4
64-65	2.3413328873651642E-4
66-67	0.0013713521197424535
68-69	0.0014800568609415505
70-71	8.194665105778075E-4
72-73	0.002909942302868133
74-75	0.00816957939627059
76-77	0.00398026590852078
78-79	0.0017559996655238733
80-81	4.6826657747303283E-4
82-83	2.006856760598712E-4
84-85	0.0015135044736181956
86-87	0.004122418262396521
88-89	0.0013379045070658082
90-91	2.0904757922903251E-4
92-93	0.002282799565181035
94-95	0.003411656493017811
96-97	0.001973409147922067
98-99	0.002082113889121164
100	0.002608913788778326
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5979500.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.86318997769858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85227665386438	46.924638008789636
2	14.256472351708451	17.639017150110945
3	4.603641733734372	8.543878894898134
4	1.968203487031768	4.870373849320604
5	1.0087372104136343	3.120185084269619
6	0.5976226144547376	2.218250479978941
7	0.37546279026207807	1.6259128146477817
8	0.24933768098806586	1.233985946205082
9	0.18989081614539402	1.057252647080047
>10	0.8466704481779564	8.914589015647978
>50	0.03619233677562934	1.528271484858589
>100	0.014648779349261525	1.6295005776349398
>500	6.161100418697622E-4	0.25825586313912435
>1k	1.6213360885777143E-4	0.17566721074347103
>5k	6.485344354083049E-5	0.26022097267504607
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6943	0.11611338740697381	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6029	0.10082782841374696	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.34476126766452E-5	0.0
5	0.0	0.0	0.0	3.34476126766452E-5	0.0
6	0.0	0.0	0.0	3.34476126766452E-5	1.67238063383226E-5
7	0.0	0.0	0.0	3.34476126766452E-5	3.34476126766452E-5
8	0.0	0.0	0.0	3.34476126766452E-5	3.34476126766452E-5
9	0.0	0.0	0.0	2.0068567605987123E-4	3.34476126766452E-5
10-11	0.0	1.67238063383226E-5	0.0	3.0939041725896814E-4	3.34476126766452E-5
12-13	0.0	1.67238063383226E-5	0.0	3.261142235972907E-4	5.017141901496781E-5
14-15	0.0	1.67238063383226E-5	0.0	3.762856426122585E-4	6.68952253532904E-5
16-17	0.0	1.67238063383226E-5	0.0	4.3481896479638765E-4	9.198093486077432E-5
18-19	0.0	1.67238063383226E-5	0.0	4.7662848064219417E-4	1.1706664436825822E-4
20-21	0.0	1.67238063383226E-5	0.0	5.853332218412912E-4	1.3379045070658084E-4
22-23	0.0	1.67238063383226E-5	0.0	8.278284137469688E-4	1.5887616021406474E-4
24-25	0.0	1.67238063383226E-5	0.0	0.0011706664436825823	1.6723806338322602E-4
26-27	0.0	1.67238063383226E-5	0.0	0.0017058282465089055	1.9232377289070992E-4
28-29	0.0	1.67238063383226E-5	0.0	0.0029851994313905847	2.0068567605987123E-4
30-31	0.0	1.67238063383226E-5	0.0	0.007049084371602976	2.0068567605987123E-4
32-33	0.0	1.67238063383226E-5	0.0	0.017835939459821055	2.1740948239819382E-4
34-35	0.0	1.67238063383226E-5	0.0	0.03631574546366753	2.4249519190567772E-4
36-37	0.0	1.67238063383226E-5	0.0	0.06932853917551635	2.675809014131616E-4
38-39	0.0	1.67238063383226E-5	0.0	0.11965047244752905	2.8430470775148424E-4
40-41	0.0	1.67238063383226E-5	0.0	0.1770298519943139	3.0102851408980686E-4
42-43	0.0	1.67238063383226E-5	0.0	0.23836441174011203	3.0939041725896814E-4
44-45	0.0	1.67238063383226E-5	0.0	0.3145747972238482	3.177523204281294E-4
46-47	0.0	1.67238063383226E-5	0.0	0.4071577891128021	3.930094489505812E-4
48-49	0.0	1.67238063383226E-5	0.0	0.5100510076093319	4.1809515845806503E-4
50-51	0.0	1.67238063383226E-5	0.0	0.6167656158541683	4.264570616272263E-4
52-53	0.0	1.67238063383226E-5	0.0	0.7261978426289823	4.7662848064219417E-4
54-55	0.0	1.67238063383226E-5	0.0	0.8512835521364662	5.184379964880007E-4
56-57	0.0	1.67238063383226E-5	0.0	0.9975248766619282	5.184379964880007E-4
58-59	0.0	1.67238063383226E-5	0.0	1.166510577807509	5.184379964880007E-4
60-61	0.0	1.67238063383226E-5	0.0	1.3446692867296597	5.518856091646459E-4
62-63	0.0	1.67238063383226E-5	0.0	1.5278200518437997	5.518856091646459E-4
64-65	0.0	1.67238063383226E-5	0.0	1.7202023580566936	5.602475123338072E-4
66-67	0.0	1.67238063383226E-5	0.0	1.9403545446943724	5.936951250104524E-4
68-69	0.0	1.67238063383226E-5	0.0	2.1842461744293002	6.020570281796137E-4
70-71	0.0	1.67238063383226E-5	0.0	2.458382807927084	6.271427376870976E-4
72-73	0.0	1.67238063383226E-5	0.0	2.731875574880843	7.860188979011623E-4
74-75	0.0	1.67238063383226E-5	0.0	3.016389330211556	7.860188979011623E-4
76-77	0.0	1.67238063383226E-5	0.0	3.32930847060791	7.860188979011623E-4
78-79	0.0	1.67238063383226E-5	0.0	3.6781001755999667	7.860188979011623E-4
80-81	0.0	1.67238063383226E-5	0.0	4.041282715946149	8.361903169161302E-4
82-83	0.0	1.67238063383226E-5	0.0	4.4198009867045736	8.696379295927753E-4
84-85	1.67238063383226E-5	1.67238063383226E-5	0.0	4.808462246007191	8.696379295927753E-4
86-87	1.67238063383226E-5	1.67238063383226E-5	0.0	5.2128773308805085	8.779998327619366E-4
88	1.67238063383226E-5	1.67238063383226E-5	0.0	5.532653231875575	8.86361735931098E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8345	0.0	44.082706	1
GTATCAA	14345	0.0	38.53249	1
CAACGCA	16165	0.0	32.30058	5
ATCAACG	16490	0.0	31.750006	3
TATCAAC	17720	0.0	30.712616	2
TCAACGC	17715	0.0	29.68663	4
AACGCAG	18140	0.0	29.50754	6
ACGCAGA	20450	0.0	25.439049	7
CGCAGAG	21030	0.0	24.51378	8
GTACATG	24515	0.0	24.490437	1
GTACTAG	3220	0.0	24.460236	1
TACATGG	25210	0.0	23.563847	2
ACATGGG	25210	0.0	22.594826	3
GCAGAGT	23785	0.0	21.121145	9
GTGGTAT	4065	0.0	20.99994	1
CATGGGG	19035	0.0	20.467915	4
CGTTAAC	1020	0.0	19.882378	1
TGGTATC	4305	0.0	18.995384	2
TAGTACT	4365	0.0	18.62661	4
TAGGCAT	4650	0.0	18.59674	5
>>END_MODULE