FastQCFastQC Report
Thu 26 May 2016
ERR580121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8009407
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150850.18834103448607367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116270.14516680198671386No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC104350.044.45614294
GTACTAG113150.039.7731171
GCATCGT114350.036.703848
GTATCAA209500.036.587761
TAGGCAT134300.036.4114535
GGTATCA126550.036.0447351
CAACGCA202600.034.9515345
CTGTGCG145500.034.920089
TAGTACT149650.034.1565674
ACTGTGC152800.033.9272278
CTAGGCA151200.033.1844024
ATCAACG215750.032.912933
GTACTGT159750.032.1569986
TACTGTG170000.031.406947
GTGGTAT25650.031.1669141
TATCAAC256250.029.895122
TCTAACG57700.029.7481632
CTAGTAC179900.029.721433
CTAACGC57350.029.4375323
CGCCTAC58800.029.1750587
Produced by FastQC (version 0.11.5)