FastQCFastQC Report
Thu 26 May 2016
ERR580121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8009407
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126290.15767709145009112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98150.12254340427449872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC96450.041.57111794
GTACTAG107500.038.9194831
GTATCAA204200.036.9261631
TACCCCG5750.036.783155
TAGGCAT131900.034.7071045
CAACGCA205500.033.5980575
GGTATCA121550.033.4538961
ATCAACG217600.031.7733783
CTAGGCA150750.031.4898784
TATTCTC31050.030.8796795
CTGTGCG140950.030.710199
TAGTACT145000.029.7564834
AGGGCTA78700.029.7412225
ACTGTGC150100.029.3391048
TATCAAC262000.028.7037872
GCATCGT139150.028.2702838
CTAACGC46300.027.8150083
GTACTGT156000.027.7173146
CTATTGA84150.027.6464279
TACTGTG167400.027.4020027
Produced by FastQC (version 0.11.5)