FastQCFastQC Report
Thu 26 May 2016
ERR580120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5362454
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192110.35825015934868626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163480.30486042397752966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92220.1719735031759713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91700.052.145211
GTATCAA165950.041.3371771
ATCAACG195250.034.3611223
CAACGCA195550.034.329895
TATCAAC204500.033.2468072
TCAACGC206000.032.636174
AACGCAG209950.032.3199736
GTGGTAT38100.030.1111721
ACGCAGA232700.028.7358557
GTACATG200900.028.2481581
CGCAGAG238950.027.9058368
TACATGG202500.027.3756072
TGGTATC40600.026.4048542
ACATGGG204100.026.1909873
CATGGGG161500.025.0646134
GCAGAGT262450.024.9278769
ATGGGGG122450.024.417455
GAGTACT159350.022.21555112-13
CAGAGTA257000.021.43530310-11
TGGGGGG120650.020.7206786
Produced by FastQC (version 0.11.5)