FastQCFastQC Report
Thu 26 May 2016
ERR580120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5362454
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170770.31845494618695097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149940.2796107901345168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85540.15951651986198856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93450.053.821031
GTATCAA163600.038.7028851
ATCAACG197600.031.0635013
CAACGCA199450.030.797215
TATCAAC210400.029.596752
TCAACGC210550.029.2190724
GTGGTAT37400.029.1416741
AACGCAG214600.028.993986
GTACATG191750.027.1156711
TGGTATC40200.026.0707022
TACATGG195550.026.0041922
ACGCAGA238900.025.6907427
CGCAGAG245200.024.8965028
ACATGGG198000.024.8290823
CATGGGG156350.023.2361184
GCAGAGT270100.022.2533599
ATGGGGG115100.021.5589925
GAGTACT168300.021.0948112-13
ATAACGC5350.021.0839333
CAGAGTA261500.020.48606310-11
Produced by FastQC (version 0.11.5)