FastQCFastQC Report
Thu 26 May 2016
ERR580119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6897702
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119530.1732895970281117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101450.14707796886557292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87650.043.6202661
GTATCAA154750.039.202041
CAACGCA162800.035.2949755
ATCAACG167600.034.289613
TATCAAC188300.031.818622
AACGCAG185350.031.8014266
GTACTAG53100.031.7057571
GTACATG252850.031.264711
CTGTGCG66350.031.168939
TACATGG255050.030.1846142
TCAACGC194100.029.9215974
TAGTACT71550.029.8393634
ACATGGG248100.029.7982793
ACGCAGA201450.028.4890167
GGGCACC64400.028.31716294
CTAGTAC79050.027.6046053
CGCAGAG208900.027.4945148
CATGGGG199000.027.4361064
GCATCGT55500.027.3541288
CTAACGC24650.027.2825223
Produced by FastQC (version 0.11.5)