FastQCFastQC Report
Thu 26 May 2016
ERR580119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6897702
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108190.15684933909873172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90680.13146407310724645No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87100.043.608561
GTATCAA145550.036.366771
CAACGCA158400.031.8073045
ATCAACG163900.030.7401683
CTGTGCG67950.029.3956839
GTACATG243150.028.9031681
TAGTACT72350.028.6475374
TATCAAC184800.028.4341132
AACGCAG186100.027.9817166
TACATGG246850.027.7793452
ACATGGG240000.027.4749453
TCTAACG23100.027.4676572
CTAACGC22250.027.4602013
TAGGCAT63250.027.1218665
GGGCACC68000.027.02904194
TAACGCC22700.026.915644
CTAGTAC79800.026.7388883
TCAACGC193350.026.3738084
CATGGGG189350.025.6650714
GTACTGT85500.025.2855646
Produced by FastQC (version 0.11.5)