FastQCFastQC Report
Thu 26 May 2016
ERR580118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10027818
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242860.24218628618907923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195570.19502747257678588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116380.11605715221397117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165850.044.2943881
GTATCAA284350.038.3061451
CAACGCA299600.034.3119935
GTACTAG104300.033.413121
GGGCACC106100.033.35587394
ATCAACG313150.032.8763053
TATCAAC349950.030.9116612
GCATCGT108650.029.9350438
AACGCAG358150.029.7637846
GTGGTAT42350.029.6511121
TAGGCAT132200.028.9910375
ACGCAGA364500.028.0454947
CTGTGCG139700.027.9921959
GTACATG413450.027.9830651
TAGTACT140900.027.6704244
CGCAGAG370150.027.5540568
TACATGG420500.027.1794552
ACATGGG409450.027.0852323
TCAACGC387750.027.0110034
CTAGGCA148050.026.9376544
Produced by FastQC (version 0.11.5)