FastQCFastQC Report
Thu 26 May 2016
ERR580118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10027818
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206800.20622631962406976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172590.17211122100540716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108490.1081890397292811No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164650.043.5968481
GTATCAA288350.039.0448151
GGGCACC107850.035.21940694
CAACGCA306050.034.568525
ATCAACG320550.033.019653
GTACTAG99950.032.4521981
TATCAAC363800.030.8393152
AACGCAG366550.029.8751146
TAGGCAT122600.029.3654045
GTGGTAT41550.029.3026261
CTGTGCG145450.028.5321069
TAGTACT147900.028.2506944
CTAACGC48050.027.877343
TAACGCC48350.027.8012984
ACGCAGA382500.027.5970697
GTACATG418500.027.5649321
TACATGG421300.027.1434882
CGCAGAG388000.027.1089738
TCAACGC395600.027.09974
TCTAACG51000.027.0951862
Produced by FastQC (version 0.11.5)