FastQCFastQC Report
Thu 26 May 2016
ERR580117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6851861
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177190.25860127635397157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146750.2141753897225878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88870.12970198899247956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111400.048.8867531
GTATCAA203050.041.5283931
CAACGCA223400.036.2759485
ATCAACG229800.035.2863663
TATCAAC254150.033.003612
AACGCAG257850.032.506986
GTACTAG59350.032.4885941
CTGTGCG66650.030.2458869
TCAACGC273200.030.162624
ACGCAGA269550.030.0170577
GCATCGT59900.029.729228
TAGGCAT74200.029.5220625
CGCAGAG273650.029.4297438
GGGCACC60800.029.37930394
TAGTACT73000.028.2689384
GTACATG283500.027.4378621
CTAGGCA80650.027.1612284
ACATGGG276900.026.7547633
TACATGG287850.026.575292
GCAGAGT298500.026.4783449
Produced by FastQC (version 0.11.5)