FastQCFastQC Report
Thu 26 May 2016
ERR580117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6851861
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152430.22246510838442288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136370.19902622075958631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80890.11805551805560563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110500.047.7840351
GTATCAA196400.039.7882541
GTACTAG56750.035.460371
CAACGCA225650.032.9730225
ATCAACG230750.032.2442553
GGGCACC62100.032.23962494
TATCAAC256950.030.3572982
TAGGCAT75150.029.7708445
CTGTGCG75200.029.4353149
AACGCAG262550.029.4295716
GTGGTAT37150.028.7297841
CTAGGCA82350.028.1952844
TAGTACT82250.027.3723934
ACGCAGA275250.026.8764277
TCAACGC280900.026.7720364
CGCAGAG276850.026.6538
CGTTAAC14600.026.4074421
CTAACGC29250.026.3530673
GCATCGT72150.026.1215558
GTACATG291000.026.0296231
Produced by FastQC (version 0.11.5)