FastQCFastQC Report
Thu 26 May 2016
ERR580116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6444146
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91310.1416944929553117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71080.11030165983203981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88100.047.1307141
GTATCAA158900.040.8093451
CAACGCA180350.034.6715935
ATCAACG180650.034.6435363
TATCAAC192300.033.378432
TCAACGC194450.032.328744
AACGCAG199250.031.6266356
ACGCAGA223850.027.7725097
CGCAGAG232050.026.5474878
GTGGTAT40400.025.7235051
GTACATG270000.024.4002131
TACATGG268350.023.7788432
GCAGAGT257750.023.1941459
ACATGGG273250.022.6624973
TGGTATC44950.021.8639742
GTACTAG32200.021.1753881
CATGGGG207000.020.5789474
GTATAAG38150.020.0914971
GAGTACT103550.019.61159512-13
CAGAGTA250250.019.31778110-11
Produced by FastQC (version 0.11.5)