FastQCFastQC Report
Thu 26 May 2016
ERR580116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6444146
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81420.12634723049415703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68940.10698081638746235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88250.047.0360531
GTATCAA158050.039.574181
CAACGCA185050.032.558545
ATCAACG186500.032.407463
TATCAAC198050.031.2054442
TCAACGC202800.029.987654
AACGCAG204850.029.9378286
ACGCAGA232650.025.7746877
CGCAGAG242000.024.5846568
GTGGTAT41250.024.4710581
GTACATG266750.023.0941311
TACATGG269150.022.4905952
GTACTAG29050.022.2452531
ACATGGG268900.021.5503883
GCAGAGT271200.021.4524429
TGGTATC46050.021.3302572
CATGGGG202100.019.766454
TAGTACT41000.019.6014564
CGTTAAC9800.018.7716061
AGAGTAC245250.018.56737110-11
Produced by FastQC (version 0.11.5)