##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580116_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6444146
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.529194869265844	34.0	31.0	34.0	31.0	34.0
2	32.7328077296821	34.0	31.0	34.0	31.0	34.0
3	32.81432047008246	34.0	31.0	34.0	31.0	34.0
4	36.27963767425505	37.0	37.0	37.0	35.0	37.0
5	36.20905795740816	37.0	37.0	37.0	35.0	37.0
6	36.238810386977576	37.0	37.0	37.0	35.0	37.0
7	36.20922120634759	37.0	37.0	37.0	35.0	37.0
8	36.20060904889492	37.0	37.0	37.0	35.0	37.0
9	37.98728877340768	39.0	38.0	39.0	35.0	39.0
10-11	37.940279363627084	39.0	38.0	39.0	35.0	39.0
12-13	37.90809705428772	39.0	38.0	39.0	35.0	39.0
14-15	39.31704356170701	41.0	39.0	41.0	36.0	41.0
16-17	39.299309249045564	41.0	39.0	41.0	36.0	41.0
18-19	39.295877219417434	41.0	39.0	41.0	36.0	41.0
20-21	39.27756137120419	41.0	39.0	41.0	36.0	41.0
22-23	39.207061649441215	41.0	39.0	41.0	36.0	41.0
24-25	39.12634645459616	40.5	39.0	41.0	35.5	41.0
26-27	38.99239418225471	40.0	38.5	41.0	35.0	41.0
28-29	38.886880976936276	40.0	38.0	41.0	35.0	41.0
30-31	38.78711919314057	40.0	38.0	41.0	35.0	41.0
32-33	38.65923568460429	40.0	38.0	41.0	35.0	41.0
34-35	38.63592988116657	40.0	38.0	41.0	34.5	41.0
36-37	38.68909255004465	40.0	38.0	41.0	35.0	41.0
38-39	38.60319901194045	40.0	38.0	41.0	35.0	41.0
40-41	38.487413072267444	40.0	38.0	41.0	34.0	41.0
42-43	38.46722296484282	40.0	38.0	41.0	34.0	41.0
44-45	38.39459417896491	40.0	38.0	41.0	34.0	41.0
46-47	38.22217102157524	40.0	38.0	41.0	34.0	41.0
48-49	38.03258073296291	40.0	37.0	41.0	33.0	41.0
50-51	37.87107345178089	40.0	37.0	41.0	33.0	41.0
52-53	37.695537701970125	40.0	37.0	41.0	33.0	41.0
54-55	37.498298300504054	40.0	36.0	41.0	33.0	41.0
56-57	37.28770212530877	39.0	36.0	41.0	32.0	41.0
58-59	37.05496717485917	39.0	35.0	41.0	32.0	41.0
60-61	36.79096912143207	39.0	35.0	40.5	31.5	41.0
62-63	36.50341216663931	38.0	35.0	40.0	31.0	41.0
64-65	36.20804719508217	37.5	35.0	40.0	31.0	41.0
66-67	35.87441020113449	37.0	35.0	40.0	31.0	41.0
68-69	35.5105930250494	36.0	35.0	39.0	30.5	41.0
70-71	35.17136793610821	36.0	34.0	39.0	30.0	40.5
72-73	34.81093258905059	35.0	34.0	38.5	30.0	40.0
74-75	34.45142498323284	35.0	34.0	37.0	29.5	39.0
76-77	33.224512604152665	34.5	32.5	36.0	28.0	39.0
78-79	33.84919987846334	35.0	34.0	36.0	29.0	38.5
80-81	33.778985144036156	35.0	34.0	36.0	29.0	37.0
82-83	33.572835717874796	35.0	34.0	35.5	29.0	37.0
84-85	33.359700804420015	35.0	34.0	35.0	29.0	36.5
86-87	33.19793685617924	35.0	34.0	35.0	29.0	36.0
88-89	33.04343764092248	35.0	33.5	35.0	29.0	36.0
90-91	32.89314666365411	35.0	33.0	35.0	29.0	36.0
92-93	32.78564483175893	35.0	33.0	35.0	29.0	35.0
94-95	32.693683228157774	35.0	33.0	35.0	29.0	35.0
96-97	32.604394748349904	35.0	33.0	35.0	28.0	35.0
98-99	32.53319834777176	35.0	33.0	35.0	27.0	35.0
100	31.25357789845233	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1728049121415367
1101	2	0.08650313969094725
1101	3	0.05547705122486235
1101	4	0.03305983592473183
1101	5	0.03464548872613449
1101	6	0.0844521380437584
1101	7	0.0048730335508508915
1101	8	0.014612715800787157
1101	9	0.08088545418863191
1101	10-11	0.05304292813016076
1101	12-13	0.008175055984779078
1101	14-15	0.04095922938135743
1101	16-17	0.04018579277191492
1101	18-19	0.006860316795354038
1101	20-21	-0.028433130244842175
1101	22-23	0.008377516599040291
1101	24-25	-0.07472430714751965
1101	26-27	-0.045035434941326
1101	28-29	-0.026946350468328717
1101	30-31	-0.06272344048679912
1101	32-33	-0.004042892220908811
1101	34-35	-0.02452399483107115
1101	36-37	0.004627423062224523
1101	38-39	0.07231671842183829
1101	40-41	0.07665744872392821
1101	42-43	0.021032908594662558
1101	44-45	-0.05887494847884511
1101	46-47	0.036394866786714886
1101	48-49	-0.008669512102152055
1101	50-51	0.004331258805287064
1101	52-53	-0.03525993477295941
1101	54-55	-0.00622840693236526
1101	56-57	-0.04190013819316363
1101	58-59	-0.056035653108232
1101	60-61	-0.09247174182436169
1101	62-63	-0.060128370444751056
1101	64-65	-0.13805578328918244
1101	66-67	-0.12156057529648479
1101	68-69	-0.12953111094395098
1101	70-71	-0.20770686161556995
1101	72-73	-0.1615479649954139
1101	74-75	-0.10265691795191856
1101	76-77	-0.09478319638197519
1101	78-79	-0.030723254087014595
1101	80-81	-0.011606025467735037
1101	82-83	0.031036544010767386
1101	84-85	-0.04045227838945209
1101	86-87	-0.08808646240709095
1101	88-89	-0.03862084435587576
1101	90-91	-0.0016775132229724932
1101	92-93	-0.0910143596713553
1101	94-95	-0.0600058954944771
1101	96-97	-0.07151145568604989
1101	98-99	-0.05780542027354585
1101	100	0.005449519266704783
1102	1	0.1312630758824369
1102	2	0.03544132368362796
1102	3	0.038804476585688974
1102	4	0.010804058380230686
1102	5	0.0040137584056623155
1102	6	0.03084941347388792
1102	7	0.017527045972407507
1102	8	0.02114133692143838
1102	9	-0.02111583753389823
1102	10-11	3.174260136660223E-4
1102	12-13	-3.272340282336472E-4
1102	14-15	0.022847090283534044
1102	16-17	-0.03029860858188016
1102	18-19	-0.04848253123350332
1102	20-21	-0.054988663527588244
1102	22-23	-0.029561360903002765
1102	24-25	-0.0479586246330328
1102	26-27	-0.08118995237406779
1102	28-29	-0.060896006706293804
1102	30-31	-0.09945399086859652
1102	32-33	-0.10476253821633463
1102	34-35	-0.10349315593515485
1102	36-37	-0.1110426209591111
1102	38-39	-0.07389694298734639
1102	40-41	-0.07355986798710745
1102	42-43	-0.037439051924621936
1102	44-45	-0.03295678717620376
1102	46-47	-0.04307211099464325
1102	48-49	-0.09905400424403155
1102	50-51	-0.09046301202112517
1102	52-53	-0.14123983052272848
1102	54-55	-0.16792190957524156
1102	56-57	-0.15848929940623435
1102	58-59	-0.16600531055406265
1102	60-61	-0.15913290208951736
1102	62-63	-0.18376050892648266
1102	64-65	-0.23976469004524859
1102	66-67	-0.2224111972368803
1102	68-69	-0.10569451352898795
1102	70-71	-0.12072274504691194
1102	72-73	-0.16111048529820238
1102	74-75	-0.1914302359598068
1102	76-77	-0.14435396052443394
1102	78-79	-0.05762093711545191
1102	80-81	-0.13189504039036848
1102	82-83	-0.1219742769238934
1102	84-85	-0.13183027593490237
1102	86-87	-0.14988031201180974
1102	88-89	-0.0923443329578717
1102	90-91	-0.17913486537013057
1102	92-93	-0.17446188928691697
1102	94-95	-0.16625598472204928
1102	96-97	-0.17544160548163745
1102	98-99	-0.16331781983257798
1102	100	-0.04783745791206684
1103	1	0.12646305094487786
1103	2	0.0848988387098899
1103	3	0.04805256976926131
1103	4	0.039932922059733755
1103	5	0.03641833442883069
1103	6	0.06299809328527317
1103	7	0.029849756110273518
1103	8	0.018238710939641578
1103	9	0.028723102121993804
1103	10-11	2.1344758756924875E-4
1103	12-13	0.01139693843731493
1103	14-15	-0.012294096128826482
1103	16-17	-0.00103019819788841
1103	18-19	-0.03228320819179231
1103	20-21	0.004869306918038774
1103	22-23	-0.02472764209328915
1103	24-25	-0.07633933894221201
1103	26-27	-0.047821965624045504
1103	28-29	-0.034643447800981164
1103	30-31	-0.08081942788084717
1103	32-33	-0.09399850478673955
1103	34-35	-0.03915463889538984
1103	36-37	0.042783469312560385
1103	38-39	0.00672918730801797
1103	40-41	0.010610332825194746
1103	42-43	0.012850574511446666
1103	44-45	-0.0012761977229232002
1103	46-47	-0.01975291833344528
1103	48-49	0.009349031630655702
1103	50-51	0.020054672172456378
1103	52-53	0.005246425717281511
1103	54-55	0.001340563598596134
1103	56-57	-0.049522408054684774
1103	58-59	-0.0058326057167974454
1103	60-61	0.0264294645407972
1103	62-63	0.013886995370903321
1103	64-65	-0.017453521251404425
1103	66-67	0.01015180360163015
1103	68-69	-0.012526731086985876
1103	70-71	0.008989858719651522
1103	72-73	0.057637528115186853
1103	74-75	-0.06427040309049659
1103	76-77	-0.06488091950728858
1103	78-79	0.035504733666371635
1103	80-81	0.11837645315333845
1103	82-83	0.09135513477291823
1103	84-85	0.05620607973042979
1103	86-87	0.08571243363163461
1103	88-89	0.09600741143727731
1103	90-91	0.053679339984554986
1103	92-93	0.003983515893601464
1103	94-95	0.025516494259498756
1103	96-97	0.04020873010070147
1103	98-99	0.08453367893583419
1103	100	0.07732180413805878
1104	1	0.13166266185426423
1104	2	0.09343876271130824
1104	3	0.04138841042988872
1104	4	0.0485253853031864
1104	5	0.040893085752188085
1104	6	0.04269652076528985
1104	7	0.07616521318257696
1104	8	0.06259370372334416
1104	9	0.06811484235269205
1104	10-11	0.057663152749164226
1104	12-13	0.05804594009164532
1104	14-15	0.07529166338027693
1104	16-17	0.051649398671919755
1104	18-19	0.04097093781398797
1104	20-21	0.044552514848611224
1104	22-23	-0.004657219494006881
1104	24-25	-0.005066149803845121
1104	26-27	0.029573682798449852
1104	28-29	-0.027019025778123762
1104	30-31	0.004913265738316852
1104	32-33	0.0049409166938829685
1104	34-35	0.013633743772970774
1104	36-37	0.0514490098654008
1104	38-39	0.07227084551382035
1104	40-41	0.10807099071293891
1104	42-43	0.08056706166220806
1104	44-45	-0.034473356619059814
1104	46-47	0.047144320549712404
1104	48-49	0.013254243923761067
1104	50-51	0.01704553825934596
1104	52-53	0.041446623064686605
1104	54-55	-0.029803925776377582
1104	56-57	0.03499913094207585
1104	58-59	0.015897101671917824
1104	60-61	-0.04015906649292589
1104	62-63	0.005064110023091928
1104	64-65	0.011335799936134094
1104	66-67	-0.06782496067977206
1104	68-69	-0.07066929465991478
1104	70-71	-0.1318455825517546
1104	72-73	-0.062410659694911885
1104	74-75	-0.02411149894900433
1104	76-77	-0.15400732974242004
1104	78-79	-0.006668470428614626
1104	80-81	0.02147052778216363
1104	82-83	0.025015448694666986
1104	84-85	-0.0132097221084706
1104	86-87	-0.025438699796758613
1104	88-89	-0.016137145806567332
1104	90-91	0.05048111028862223
1104	92-93	0.03056787199083999
1104	94-95	-0.013089181579843512
1104	96-97	-0.007406129325218558
1104	98-99	0.05149472911659103
1104	100	0.06871360186625353
1105	1	0.11315467646174682
1105	2	0.08416735621243987
1105	3	-0.0010522857944295083
1105	4	0.044939911282718015
1105	5	0.015199159686531516
1105	6	0.01276778139794743
1105	7	-0.016047576408027453
1105	8	-0.03326927963539106
1105	9	-0.04662041942425077
1105	10-11	-0.025382852841133285
1105	12-13	0.0024822674070676953
1105	14-15	0.03705518990066281
1105	16-17	0.01658912829051218
1105	18-19	-0.03911006523593841
1105	20-21	-0.03401013498457672
1105	22-23	-0.004492440222804817
1105	24-25	-0.032789202922096194
1105	26-27	-0.008777982519809768
1105	28-29	0.012446796980277952
1105	30-31	-0.006586502201805899
1105	32-33	0.00714599765036894
1105	34-35	-0.018503847989386202
1105	36-37	-0.05042252692968674
1105	38-39	-0.025450432700260706
1105	40-41	-0.01000176136069797
1105	42-43	-0.00767528781176452
1105	44-45	0.057277641275639724
1105	46-47	0.0010497056796978654
1105	48-49	0.006371834211073235
1105	50-51	6.879712450782449E-4
1105	52-53	-0.04341041799687417
1105	54-55	-0.03457931193443642
1105	56-57	-0.037024077280065626
1105	58-59	-0.04810872664399568
1105	60-61	-0.11889212947465211
1105	62-63	-0.015572130726177136
1105	64-65	0.019739682450513385
1105	66-67	0.029459016908383262
1105	68-69	0.044196524075623245
1105	70-71	-0.006807355995768205
1105	72-73	-0.103448147980167
1105	74-75	-0.09053817175426815
1105	76-77	-0.10358780609466578
1105	78-79	0.014643835598384669
1105	80-81	0.04293941834973225
1105	82-83	-0.02060899350492207
1105	84-85	0.02926645357463542
1105	86-87	0.042463536694235415
1105	88-89	0.05559901403834999
1105	90-91	0.05985751177380649
1105	92-93	0.04808122195202458
1105	94-95	-0.013831223751417099
1105	96-97	-0.07212461984093466
1105	98-99	-0.10506671720867189
1105	100	0.026793550403343858
1106	1	0.10543745464688214
1106	2	0.0453568633002277
1106	3	0.034546783866986175
1106	4	0.018205440543873408
1106	5	0.025937709763567796
1106	6	0.025418251033300976
1106	7	0.04817707106994362
1106	8	0.02507454272821974
1106	9	-0.03684129020739846
1106	10-11	-0.03157825687507909
1106	12-13	-0.0256772948216053
1106	14-15	0.032819666119280555
1106	16-17	-0.040660785492924845
1106	18-19	-0.037059644943418846
1106	20-21	-0.04743756556725742
1106	22-23	-0.053584471034291425
1106	24-25	-0.034278238171403075
1106	26-27	-0.029058859479100363
1106	28-29	-0.010054829152871037
1106	30-31	-0.05468075279659246
1106	32-33	0.007475191300827078
1106	34-35	0.042985085152274394
1106	36-37	0.04529491078304204
1106	38-39	-0.002184114634601997
1106	40-41	-0.006217971895985386
1106	42-43	-0.05205539601090692
1106	44-45	0.004865893322552495
1106	46-47	-0.028854655337589463
1106	48-49	-0.058589379604079284
1106	50-51	-0.11560820337039246
1106	52-53	-0.04872953610698971
1106	54-55	-0.11052852663009105
1106	56-57	-0.11639856633942003
1106	58-59	-0.12934313855597424
1106	60-61	-0.15826429489528238
1106	62-63	-0.09864261737762803
1106	64-65	-0.14996715869694555
1106	66-67	-0.13193442259297683
1106	68-69	-0.11604048891348384
1106	70-71	-0.0696135006863372
1106	72-73	-0.025103359273536796
1106	74-75	-0.09039401370488775
1106	76-77	-0.07780615601497942
1106	78-79	-0.046026120682064686
1106	80-81	-0.024100157375542608
1106	82-83	-0.03204162256231058
1106	84-85	-0.02508138401093163
1106	86-87	-0.00979737251818591
1106	88-89	-0.01463335719264336
1106	90-91	0.013537841362548875
1106	92-93	-0.035916052509897156
1106	94-95	-0.057254555770221316
1106	96-97	-0.023981178200827458
1106	98-99	0.019487780720247372
1106	100	0.00786024131827645
1107	1	0.10222152621322778
1107	2	0.06726961071035475
1107	3	0.021155326376330663
1107	4	0.02207691307474846
1107	5	0.06899322307996414
1107	6	-0.0021016297877523016
1107	7	0.031705157654386085
1107	8	0.02855190084841297
1107	9	-0.01206237765536855
1107	10-11	-0.01426087289530642
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2316	7	-0.020052486060308183
2316	8	0.027300267118228305
2316	9	-0.019363276831896314
2316	10-11	-0.011354975754244379
2316	12-13	-0.005370805884126639
2316	14-15	0.07151200035899308
2316	16-17	0.0384537268246774
2316	18-19	0.012258829154852435
2316	20-21	0.02928861026313001
2316	22-23	0.043791703318404984
2316	24-25	0.014882214304250851
2316	26-27	0.049198348921279944
2316	28-29	0.12086678469010792
2316	30-31	-0.3288572413301054
2316	32-33	-0.08707101012080898
2316	34-35	-0.19980440503923802
2316	36-37	-0.47178174209820867
2316	38-39	-0.3562356259674786
2316	40-41	-0.24016572713642148
2316	42-43	-0.38925741737611474
2316	44-45	-0.2548471574229083
2316	46-47	-0.3478821134580343
2316	48-49	-0.3358801007422798
2316	50-51	-0.3056245266608144
2316	52-53	-0.2678826945371213
2316	54-55	-0.1564209109540684
2316	56-57	-0.8918024603384538
2316	58-59	-0.7997464952508793
2316	60-61	-0.4127706763544481
2316	62-63	-0.16255417738177158
2316	64-65	-0.09426621989680228
2316	66-67	-0.04338403675703262
2316	68-69	-0.10593706202365638
2316	70-71	-0.20631745118755163
2316	72-73	-0.0809812936579064
2316	74-75	-0.049960425018753085
2316	76-77	-0.0683856277973689
2316	78-79	-0.06426962848315299
2316	80-81	-0.10964618689482819
2316	82-83	-0.23150117506011014
2316	84-85	-0.05899001747823718
2316	86-87	-0.18779408460169833
2316	88-89	-0.21320778968319587
2316	90-91	-0.14210874621854686
2316	92-93	-0.16027762679261315
2316	94-95	-0.011757302010714454
2316	96-97	-0.14174114726890252
2316	98-99	-0.009162573410826269
2316	100	-0.337773705844576
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	1.0
11	3.0
12	8.0
13	36.0
14	219.0
15	664.0
16	1445.0
17	3026.0
18	5649.0
19	8766.0
20	13159.0
21	19627.0
22	26388.0
23	28593.0
24	29057.0
25	32311.0
26	38651.0
27	48264.0
28	60439.0
29	74960.0
30	92780.0
31	115706.0
32	147195.0
33	193269.0
34	284145.0
35	436080.0
36	794984.0
37	1663409.0
38	1981661.0
39	343607.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59046978931404	19.537680957876667	14.274246031233117	23.597603221576176
2	15.911021492778376	20.384604999473936	39.083564267445134	24.620809240302552
3	19.230174163481923	25.243765404826785	29.664402701240206	25.86165773045109
4	13.462622646103405	17.169267334869737	38.19451093559344	31.173599083433412
5	14.280626360604288	37.52331688555312	33.81342580838595	14.382630945456645
6	31.036866638341216	36.28047843732901	18.28329153312169	14.399363391208084
7	28.010004121571463	32.11948708781181	21.535909191352644	18.334599599264077
8	26.91400724132559	33.856611077005965	19.88669057268296	19.342691108985488
9	26.316611133949074	16.423469521562012	20.415478148570077	36.84444119591884
10-11	25.664876953779814	25.72435731761715	27.512942565602394	21.097823163000637
12-13	26.333792768688213	23.1120605349789	27.52348567117236	23.030661025160526
14-15	23.73944009979314	24.69168648896853	25.21127559412399	26.357597817114346
16-17	23.297340420496827	27.49358758400463	25.8228678804104	23.386204115088145
18-19	22.846631332446265	26.997136210292744	27.583456980344838	22.572775476916153
20-21	24.176933626768157	26.043499405816206	27.326984510253823	22.452582457161814
22-23	23.986941305005146	25.885310760458097	26.958852742931928	23.168895191604832
24-25	23.579485939641962	26.027552758736377	27.28256001648628	23.110401285135378
26-27	23.474570550457365	26.321829359226136	26.917460492349086	23.286139597967413
28-29	23.2054807616649	26.278410612019005	27.230212382851253	23.285896243464833
30-31	23.747362716754598	25.999603190275693	27.158436563478812	23.0945975294909
32-33	23.618607301161283	26.189131283843437	26.857946940955635	23.334314474039648
34-35	23.80298885451506	26.28016248946689	26.993213102798748	22.923635553219306
36-37	23.717836644971904	26.105439939918618	26.760651691794862	23.416071723314616
38-39	23.68910179655588	26.198424310129337	26.926416124227547	23.18605776908723
40-41	23.879999137073003	25.707565215785294	27.116417698260193	23.29601794888151
42-43	23.571458889061926	25.77320523573445	26.99295069021501	23.66238518498861
44-45	23.74333386431456	26.06083027514785	26.840022597609313	23.355813262928272
46-47	23.62739408504669	25.964150053396956	26.489770388049944	23.91868547350641
48-49	23.83737722626219	25.90262219949389	26.756072823440945	23.503927750802973
50-51	23.813859777736532	26.05070947894158	26.487619888874477	23.64781085444741
52-53	23.673156159068864	26.141315227934044	26.439712900096996	23.745815712900097
54-55	23.56667333199406	26.045492294951366	26.578656411218578	23.809177961835996
56-57	23.854133973812612	26.067473007421864	26.308428636437874	23.769964382327643
58-59	23.507012344081225	26.14553419829501	26.715691352113456	23.631762105510305
60-61	23.676685512782036	26.277000963048184	26.460610149838875	23.5857033743309
62-63	23.6549431793009	26.05684296757966	26.405201109575422	23.883012743544025
64-65	23.379025647885403	25.978815099288994	26.602663079726348	24.039496173099252
66-67	23.60610907926198	26.01384254121012	26.461550255367435	23.918498124160465
68-69	23.425554457638842	25.953959657962088	26.603856519743687	24.016629364655383
70-71	23.400577692610952	25.70443481925167	26.55839630904902	24.33659117908836
72-73	23.395907057252423	25.940411817573565	26.532991317198444	24.130689807975564
74-75	23.471631011131784	26.05073440192725	26.4416818827246	24.035952704216363
76-77	23.29229716590275	26.06127825234174	26.26596895948451	24.380455622271
78-79	23.27353867958178	25.954750496448142	26.39788182124949	24.373829002720584
80-81	23.24046228539235	26.247617108603517	26.326922030947774	24.184998575056355
82-83	23.504869156217243	25.950472794253997	26.672222105837324	23.872435943691432
84-85	23.46880629716664	25.96105456188128	26.39834228242806	24.171796858524022
86-87	22.95857440881384	26.25158154257374	26.60997658704005	24.17986746157238
88-89	23.229076790336496	25.72229533025866	26.633974959807823	24.414652919597025
90-91	23.217655198013137	25.860385664132657	26.543914652256035	24.378044485598174
92-93	23.169199840006023	25.857781491831343	26.642706365657627	24.330312302505007
94-95	22.786094728480872	25.844373940228603	26.837002396762273	24.53252893452825
96-97	22.83609192043581	25.846746977182523	26.74245206357296	24.574709038808713
98-99	22.652200641571714	25.891801831419325	26.681091930446815	24.774905596562142
100	22.92099529966516	25.634725215312393	26.54446944440953	24.899810040612916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	93.0
1	78.5
2	123.5
3	205.0
4	159.0
5	126.0
6	261.0
7	546.0
8	880.0
9	1053.5
10	1019.0
11	993.5
12	1073.0
13	1240.5
14	1433.5
15	1676.5
16	1997.0
17	2298.5
18	2664.0
19	3039.5
20	3485.0
21	4031.0
22	4663.0
23	5517.5
24	6578.5
25	7850.5
26	9599.0
27	11921.0
28	14884.5
29	19478.0
30	25706.0
31	33430.0
32	44452.0
33	58069.0
34	73042.0
35	90620.5
36	113816.5
37	140744.5
38	162640.5
39	181118.0
40	194724.0
41	203731.0
42	216238.5
43	232793.0
44	250451.0
45	272809.5
46	291191.5
47	303850.5
48	314359.5
49	315350.5
50	307398.0
51	298588.5
52	288629.0
53	276423.5
54	264011.0
55	240765.0
56	214314.0
57	184343.5
58	156090.5
59	136638.0
60	111860.0
61	87119.0
62	67863.5
63	51284.5
64	36929.0
65	25132.0
66	17773.0
67	13183.0
68	10030.0
69	7642.0
70	5824.0
71	4444.0
72	3176.5
73	2224.0
74	1610.5
75	1073.5
76	705.0
77	430.5
78	246.5
79	141.0
80	81.5
81	42.5
82	24.5
83	17.5
84	13.0
85	5.5
86	3.5
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35214906676540225
2	4.345028805989188E-4
3	0.0022190682830587637
4	0.0015052421220748257
5	0.004934711286802006
6	0.0
7	3.1035920042779914E-5
8	3.41395120470579E-4
9	0.0030570381242138213
10-11	3.879490005347489E-5
12-13	6.207184008555983E-5
14-15	2.405283803315443E-4
16-17	1.086257201497297E-4
18-19	9.698725013368722E-4
20-21	1.8621552025667947E-4
22-23	1.6293858022459453E-4
24-25	0.0
26-27	0.002188032363015984
28-29	0.011405700615721617
30-31	0.003902766945379574
32-33	0.014982590400652003
34-35	0.012018660036566521
36-37	0.013741153598940806
38-39	0.013547179098673433
40-41	0.014881723660512967
42-43	0.007223610389957024
44-45	0.0015750729421710806
46-47	0.006618409949122816
48-49	0.01382650237905845
50-51	0.004065705525604169
52-53	0.006145112168470423
54-55	0.005454562947518569
56-57	2.094924602887644E-4
58-59	7.06067180973243E-4
60-61	3.7243104051335894E-4
62-63	9.233186212727023E-4
64-65	4.345028805989188E-4
66-67	0.0015207600820962158
68-69	0.0019164680626416598
70-71	9.543545413154824E-4
72-73	0.003111350984288686
74-75	0.00883747823218158
76-77	0.004306233905935713
78-79	0.0018543962225560998
80-81	4.888157406737836E-4
82-83	1.706975602352895E-4
84-85	0.0016293858022459454
86-87	0.0043295108459677975
88-89	0.0016449037622673353
90-91	2.405283803315443E-4
92-93	0.0023820068632833583
94-95	0.0037941412252298443
96-97	0.0018078423424919298
98-99	0.0019785399027272194
100	0.002125960522930424
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6444146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.24460569600697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00655444730836	46.68753406188787
2	14.897813401001523	18.546170417560568
3	4.814110880286928	8.989573005609506
4	2.089375986077666	5.2020953761644035
5	1.0010862420730506	3.1156109202767213
6	0.5998429027173707	2.240219097551461
7	0.35917021117864245	1.564948572079631
8	0.2455213127756826	1.2225901842950688
9	0.17469146074914232	0.978624098351086
>10	0.7661537065218671	8.032547098772849
>50	0.03198305106474098	1.346609609173424
>100	0.012977877200048697	1.4219259055663331
>500	5.388922635849753E-4	0.21517887582326967
>1k	1.1975252095266104E-4	0.15617885482672184
>5k	5.987626045672108E-5	0.28019392206108373
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8142	0.12634723049415703	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6894	0.10698081638746235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5517960021389957E-5	0.0
2	0.0	0.0	0.0	1.5517960021389957E-5	0.0
3	0.0	0.0	0.0	4.655388006416987E-5	0.0
4	0.0	0.0	0.0	6.207184008555983E-5	0.0
5	0.0	0.0	0.0	7.758980010694978E-5	0.0
6	0.0	0.0	0.0	7.758980010694978E-5	1.5517960021389957E-5
7	0.0	0.0	0.0	1.086257201497297E-4	1.5517960021389957E-5
8	0.0	0.0	0.0	1.2414368017111966E-4	1.5517960021389957E-5
9	0.0	0.0	0.0	2.3276940032084935E-4	4.655388006416987E-5
10-11	0.0	0.0	0.0	3.3363614045988407E-4	6.207184008555981E-5
12-13	0.0	0.0	0.0	3.56913080491969E-4	7.758980010694978E-5
14-15	0.0	0.0	0.0	3.957079805454439E-4	1.2414368017111966E-4
16-17	0.0	0.0	0.0	4.7329778065239366E-4	1.2414368017111966E-4
18-19	0.0	0.0	0.0	5.664055407807334E-4	1.2414368017111966E-4
20-21	0.0	0.0	0.0	6.672722809197681E-4	1.2414368017111966E-4
22-23	0.0	0.0	0.0	7.215851409946329E-4	1.2414368017111966E-4
24-25	0.0	0.0	0.0	0.0010164263814010422	1.2414368017111966E-4
26-27	0.0	0.0	0.0	0.001427652321967876	1.2414368017111966E-4
28-29	0.0	0.0	0.0	0.0023664889032619686	1.2414368017111966E-4
30-31	0.0	0.0	0.0	0.0057881990879784535	1.2414368017111966E-4
32-33	0.0	0.0	0.0	0.014641195280181422	1.2414368017111966E-4
34-35	0.0	0.0	0.0	0.029437570160576746	1.6293858022459453E-4
36-37	0.0	0.0	0.0	0.05487150663563489	1.8621552025667947E-4
38-39	0.0	0.0	0.0	0.09369744260915255	2.3276940032084935E-4
40-41	0.0	0.0	0.0	0.13934352201207112	2.638053203636293E-4
42-43	0.0	0.0	0.0	0.18828716791953504	2.638053203636293E-4
44-45	0.0	0.0	0.0	0.25073143904560824	2.7932328038501923E-4
46-47	0.0	0.0	0.0	0.3262961453697666	2.7932328038501923E-4
48-49	0.0	0.0	0.0	0.41169923834748623	2.870822603957142E-4
50-51	0.0	0.0	0.0	0.5014085652311415	2.9484124040640914E-4
52-53	0.0	0.0	0.0	0.5929878062973744	3.0260022041710415E-4
54-55	0.0	0.0	0.0	0.6964072508599277	3.1811818043849405E-4
56-57	0.0	0.0	0.0	0.8202793667306731	3.2587716044918906E-4
58-59	0.0	0.0	0.0	0.9612289976049581	3.3363614045988407E-4
60-61	0.0	0.0	0.0	1.1120868459529005	3.41395120470579E-4
62-63	0.0	0.0	0.0	1.264496490302982	3.56913080491969E-4
64-65	0.0	0.0	0.0	1.4232917752018652	3.56913080491969E-4
66-67	0.0	0.0	0.0	1.6103840601997534	3.64672060502664E-4
68-69	0.0	0.0	0.0	1.8227861379925283	3.7243104051335894E-4
70-71	0.0	0.0	0.0	2.0534218188104365	3.879490005347489E-4
72-73	0.0	0.0	0.0	2.2908776430577458	4.4226186060961374E-4
74-75	0.0	0.0	0.0	2.5315922389095467	4.5002084062030875E-4
76-77	0.0	0.0	0.0	2.799944321559443	4.5002084062030875E-4
78-79	0.0	0.0	0.0	3.0956545677270504	4.5002084062030875E-4
80-81	0.0	0.0	0.0	3.4136718814254055	4.655388006416987E-4
82-83	0.0	0.0	0.0	3.7504659267496425	4.888157406737837E-4
84-85	0.0	0.0	0.0	4.09083686185881	4.965747206844786E-4
86-87	0.0	0.0	0.0	4.44810685543127	4.965747206844786E-4
88	0.0	0.0	0.0	4.73524342868706	5.120926807058685E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8825	0.0	47.036053	1
GTATCAA	15805	0.0	39.57418	1
CAACGCA	18505	0.0	32.55854	5
ATCAACG	18650	0.0	32.40746	3
TATCAAC	19805	0.0	31.205444	2
TCAACGC	20280	0.0	29.98765	4
AACGCAG	20485	0.0	29.937828	6
ACGCAGA	23265	0.0	25.774687	7
CGCAGAG	24200	0.0	24.584656	8
GTGGTAT	4125	0.0	24.471058	1
GTACATG	26675	0.0	23.094131	1
TACATGG	26915	0.0	22.490595	2
GTACTAG	2905	0.0	22.245253	1
ACATGGG	26890	0.0	21.550388	3
GCAGAGT	27120	0.0	21.452442	9
TGGTATC	4605	0.0	21.330257	2
CATGGGG	20210	0.0	19.76645	4
TAGTACT	4100	0.0	19.601456	4
CGTTAAC	980	0.0	18.771606	1
AGAGTAC	24525	0.0	18.567371	10-11
>>END_MODULE