FastQCFastQC Report
Thu 26 May 2016
ERR580115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7437721
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90460.12162327680750595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74450.10009786599954475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74800.041.301971
GTATCAA119350.036.089431
GTACATG226450.032.705081
CAACGCA129150.031.624795
TACATGG232300.031.468362
ACATGGG232100.030.677253
ATCAACG135000.030.2875673
AACGCAG146250.028.618736
TATCAAC149150.028.3038832
CATGGGG189150.028.0039864
GAGTACA153550.027.9287831
TCAACGC147200.027.8746054
GTACTAG35200.026.8509941
ATGGGGG134600.026.4334055
AGTACAT156600.025.9066932
ACGCAGA165650.024.5307437
TGGGGGG120400.024.2288956
CTAACGC16350.024.1457353
TAACGCC17150.023.2946994
TCTAACG17450.023.1683942
Produced by FastQC (version 0.11.5)