FastQCFastQC Report
Thu 26 May 2016
ERR580115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7437721
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80460.10817829816418228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74050.040.0010721
GTATCAA115300.037.3119471
CAACGCA124750.033.0040935
GTACATG221050.032.181371
TACATGG224100.031.4911942
ATCAACG131200.031.4538423
ACATGGG226100.030.3504623
AACGCAG141800.029.497156
TCAACGC144200.029.0737424
TATCAAC148350.028.5870462
CATGGGG185750.027.3272724
GTACTAG35500.027.1507381
ATGGGGG131400.025.610735
ACGCAGA163100.025.2415737
GAGTACA154700.025.1953471
TGGGGGG112500.025.1483946
CTAACGC15500.024.562153
TAACGCC15900.024.5347884
AGTACAT154050.024.1110252
TCTAACG16850.023.7175222
Produced by FastQC (version 0.11.5)