FastQCFastQC Report
Thu 26 May 2016
ERR580114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5490570
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60200.1096425325603717No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69500.041.606471
GTATCAA122300.039.3296171
CAACGCA136650.034.0249755
ATCAACG140100.033.0552863
TATCAAC151050.031.5322762
AACGCAG154600.030.5169736
TCAACGC152950.030.4612164
GTACTAG27950.028.0934561
ACGCAGA175350.026.2345967
CGCAGAG182150.025.0744048
GTACATG224800.024.8897881
TACATGG227850.024.0611322
ACATGGG233250.022.7570063
GTGGTAT32350.021.6562141
GCAGAGT206700.021.6455759
CATGGGG186800.020.8389824
TAGTACT38400.020.3235664
CTAGTAC38050.020.0171953
GCATCGT31950.020.0014538
TAGGCAT40150.019.0859245
Produced by FastQC (version 0.11.5)