FastQCFastQC Report
Thu 26 May 2016
ERR580114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5490570
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55270.10066350123939773No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67100.044.8465351
GTATCAA116600.039.156831
CAACGCA133500.033.0914765
ATCAACG138200.031.8995193
TATCAAC147050.030.7136822
AACGCAG151900.029.8244886
TCAACGC150250.029.5903574
GTACTAG26200.027.5436121
ACGCAGA175450.024.9641237
GTGGTAT29750.024.5739781
GTACATG223900.023.9306771
CGCAGAG182700.023.7934238
TACATGG226150.023.379192
ACATGGG231850.021.9538523
TAGTACT35800.020.8731334
GCAGAGT209650.020.2642849
CATGGGG184300.019.7883664
TGGTATC35750.019.7191752
TAGGCAT42400.019.286485
CTAGTAC38150.018.848913
Produced by FastQC (version 0.11.5)