FastQCFastQC Report
Thu 26 May 2016
ERR580113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6467995
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99110.1532314109704785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77950.12051648153716878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80100.040.868711
GTATCAA138550.037.444081
CAACGCA138150.034.676275
GTACTAG57550.034.32561
ATCAACG148950.032.318793
GTACATG257450.031.3683451
GCATCGT59850.030.6175848
TAGGCAT69850.030.4888385
GGGCACC63650.030.20743294
CTGTGCG74050.030.2050279
TACATGG264200.030.1998772
ACATGGG256900.030.157043
AACGCAG167750.029.8846956
TATCAAC173300.029.490842
TAGTACT75550.029.2470844
TCTAACG25000.028.7744852
CATGGGG200650.028.4679574
CTAACGC24950.028.4512943
GAGTACA177100.028.3374021
CTAGGCA77800.028.038684
Produced by FastQC (version 0.11.5)