FastQCFastQC Report
Thu 26 May 2016
ERR580113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6467995
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87260.13491043205815714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72040.11137918319355535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79600.039.398771
GTATCAA135450.037.1428031
GTACTAG54550.033.6155131
CAACGCA141600.032.695535
ATCAACG148800.031.2412873
GTACATG249750.030.4044341
TACATGG255500.029.4091632
ACATGGG249350.029.2184963
TAGGCAT69550.028.5187645
TATCAAC174350.028.372042
AACGCAG171900.028.2704816
CTAACGC23850.027.5914943
CTAGGCA76050.027.2557494
CATGGGG196900.026.9982514
TAGTACT71100.026.7734414
TCTAACG24850.026.4910032
GAGTACA174600.026.3907491
CTGTGCG69950.026.3356029
TAACGCC25000.026.3212664
ATGGGGG158900.026.2074785
Produced by FastQC (version 0.11.5)