FastQCFastQC Report
Thu 26 May 2016
ERR580112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6908347
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181210.26230587432854774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154060.22300559019400734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83090.12027479221874639No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102600.050.6425931
GTATCAA180900.039.977791
CAACGCA214050.032.969315
ATCAACG215550.032.6990973
TATCAAC227250.031.5344472
TCAACGC228050.031.0909884
AACGCAG231050.030.8340136
ACGCAGA259900.026.9231117
GTGGTAT45800.026.1803651
CGCAGAG268750.025.9662068
GTACATG232050.025.5525671
TACATGG233950.024.8628442
ACATGGG236850.023.6241263
GTACTAG30050.023.1589181
GCAGAGT295250.022.8427899
TGGTATC51750.022.0800512
CATGGGG184450.021.3358554
GAGTACT166750.020.96063612-13
CTAGTAC40300.020.418093
CAGAGTA292200.019.6407810-11
Produced by FastQC (version 0.11.5)