FastQCFastQC Report
Thu 26 May 2016
ERR580112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6908347
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168070.2432854053219967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150440.21776555230940195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84420.12219999950784176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107150.051.3215331
GTATCAA186050.040.355861
CAACGCA226550.032.320555
ATCAACG226950.032.2235683
TATCAAC238350.031.3326172
TCAACGC239800.030.5551824
AACGCAG244950.030.331596
ACGCAGA272050.026.7228247
GTGGTAT43300.026.35851
CGCAGAG281050.025.766768
GTACATG232300.023.8145311
TACATGG234900.022.969252
GTACTAG28550.022.9615881
GCAGAGT313550.022.6315369
ACATGGG236900.021.863053
TGGTATC50400.021.7276782
GAGTACT177850.020.47812312-13
TAGTACT42550.020.102954
TCTAACG14600.019.9584472
CATGGGG184550.019.8640824
Produced by FastQC (version 0.11.5)