FastQCFastQC Report
Thu 26 May 2016
ERR580111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3533701
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76140.2154681451543297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58400.1652658218677811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37520.10617763076162924No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107950.034.289431
GTACTAG27850.032.931961
GGTATCA68500.032.2025641
CAACGCA116950.030.1609235
ATCAACG120500.029.3134673
TAGTACT37350.028.4590134
GGGCACC32600.028.25784994
TATCAAC131600.028.0573442
CTGTGCG37850.027.8144479
AACGCAG132650.027.5659486
GTACATG134700.027.4449921
TACATGG135950.026.8482072
ACATGGG130800.026.7174743
GCATCGT31850.026.4127398
TCAACGC137150.026.097024
CTAGTAC42350.025.9882343
CTAACGC13000.025.6879433
TCTAACG13250.025.5593262
ACGCAGA139150.025.3648747
TAACGCC13750.025.3122674
Produced by FastQC (version 0.11.5)