FastQCFastQC Report
Thu 26 May 2016
ERR580111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3533701
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64620.18286776385438383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50790.1437303269291884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37460.10600783710902535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG29500.036.809621
GTATCAA100600.032.00841
TAGGCAT38550.031.332875
CTAGGCA40900.029.877314
GGTATCA66650.029.6224161
CTAACGC13750.028.7123013
GTACATG128500.028.3145181
GGGCACC30550.028.31168294
GCATCGT35700.028.1728558
CAACGCA109700.028.019645
TCTAACG14800.027.6299252
TACATGG129700.027.505782
ATCAACG112050.027.4739363
TAACGCC14200.027.4714264
ACATGGG126150.027.1228663
CTGTGCG36100.026.6893589
TAGTACT37400.026.3899844
CGCCTAC14850.025.9517447
TATCAAC124950.025.6925142
AACGCAG125050.025.4439266
Produced by FastQC (version 0.11.5)