FastQCFastQC Report
Thu 26 May 2016
ERR580110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9814350
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260760.265692582799676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206940.2108545140534014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116720.11892789639660294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147050.050.3299141
GTATCAA264150.042.3655471
CAACGCA315000.034.461625
ATCAACG319450.033.9995733
TATCAAC332750.033.264032
AACGCAG344400.032.1207926
TCAACGC341000.031.9879864
GTGGTAT58650.028.7814661
ACGCAGA381750.028.349597
CGCAGAG395100.027.3201838
GTACTAG40900.026.3268361
GTACATG350550.025.2975351
GCAGAGT435750.024.0732759
TACATGG363450.023.8304062
ACATGGG360300.023.4633393
TGGTATC73900.021.3150752
GAGTACT230900.021.29566812-13
CATGGGG283250.021.1969934
CAGAGTA420150.020.44953210-11
TAGTACT60650.020.3106484
Produced by FastQC (version 0.11.5)