FastQCFastQC Report
Thu 26 May 2016
ERR580110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9814350
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232150.23654139092247578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197230.2009608379566655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110930.11302837172100037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145550.052.119011
GTATCAA263500.040.6233941
CAACGCA315400.033.1251075
ATCAACG317000.032.9729083
TATCAAC333950.031.9458962
AACGCAG345000.030.8259326
TCAACGC341700.030.7543374
GTGGTAT59100.028.6569041
ACGCAGA386150.026.9324687
CGCAGAG401850.025.7282058
GTACTAG43700.025.693181
GTACATG354850.024.4621891
TGGTATC68050.023.8265762
TACATGG358500.023.7148362
GCAGAGT444400.022.9581349
TAGTACT63300.022.4966834
ACATGGG360500.022.4366173
CTAGTAC65300.022.0236853
GAGTACT238350.021.20478412-13
CTGTGCG66300.020.201439
Produced by FastQC (version 0.11.5)